환경생물 (Korean Journal of Environmental Biology)

  • 제39권4호
  • /
  • Pages.479-485
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  • 2021
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  • 1226-9999(pISSN)
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  • 2287-7851(eISSN)
한국환경생물학회 (Korean Society of Environmental Biology)
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고추에서 탄저병균 Colletotrichum scovillei를 검출하기 위한 반선택 배지

A semi-selective agar medium to detect the presence of the causal agent of anthracnose, Colletotrichum scovillei, in chilli pepper

  • 홍성기 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 박세근 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 최효원 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 류현주 (농촌진흥청 국립농업과학원 작물보호과)
  • Sung Kee Hong (Division of Crop Protection, National Institute of Agricultural Science, RDA) ;
  • Se-Keun Park (Division of Crop Protection, National Institute of Agricultural Science, RDA) ;
  • Hyo-Weon Choi (Division of Crop Protection, National Institute of Agricultural Science, RDA) ;
  • Hyunjoo Ryu (Division of Crop Protection, National Institute of Agricultural Science, RDA)
  • 투고 : 2021.11.02
  • 심사 : 2021.11.29
  • 발행 : 2021.12.31
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초록

고추에서 Colletotrichum scovillei를 검출하고 계수하기 위한 반선택배지가 개발되었다. 기본배지로서 Potato-dextrose-agar (PDA)가 사용되었다. 반선택배지의 최종 조성은 부생성곰팡이와 세균을 저해하고, C. scovillei의 생장에 양호할 수 있도록 확립되었다. 반선택배지는 pyribencarb와 pydiflumetofen 각각 40 ㎍ mL-1와 세균을 방지하기 위한 streptomycin 100 ㎍ mL-1를 포함한다. 배지의 pH는 85% lactic acid로 4.8로 조절했다. 반선택배지에 배양할 때 C. scovillei의 균사생육 저해율은 Fusarium속과 다른 부생균보다 뚜렷이 낮았다. 반선택배지에 과실과 줄기조직 현탁액을 평판배양하여 탄저병균이 자연 감염된 식물체로부터 C. scovillei가 검출되었고, 신뢰할 수 있고 정량할 수 있었다. 이것은 자연 감염된 고추조직에서 C. scovillei의 존재를 검출하기 위한 반선택성배지에 대한 처음 보고이다.

A semi-selective agar medium was developed for detecting and enumerating Colletotrichum scovillei in chili (Capsicum annum) plant material. Potato-dextrose-agar(PDA) was used as the basic medium. The composition of the semi-selective agar medium was established after several attempts to favor the development of C. scovillei and inhibit the growth of other fungi and bacteria. The semi-selective agar medium contained PDA amended with pyribencarb and pydiflumetofen at 40 ㎍ mL-1 each and streptomycin at 100㎍ mL-1 for preventing bacterial growth. The pH was adjusted to 4.8 with 85% lactic acid. The inhibition of the mycelial growth of C. scovillei was significantly less than that of most other fungi including Fusarium species when grown on the semi-selective medium. C. scovillei was detected from naturally infected chili plants by plating fruit and stem tissue suspensions on the semi-selective medium, which was found to be reliable and quantifiable. This was the first report of a semi-selective agar medium to detect the presence of C. scovillei in naturally infected chili tissue.

키워드

과제정보

본 논문은 농촌진흥청 어젠다 과제(과제번호: PJ01530002)의 지원에 의한 연구 결과의 일부입니다.

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