Parasites, Hosts and Diseases

  • 제59권3호
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  • Pages.257-263
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  • 2021
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Evaluation of Rapid IgG4 Test for Diagnosis of Gnathostomiasis

  • Wang, Yue (Institute of Parasitic Diseases, School of Basic Medical Sciences and Forensic Medicine, Hangzhou Medical College) ;
  • Ma, An (Institute of Parasitic Diseases, School of Basic Medical Sciences and Forensic Medicine, Hangzhou Medical College) ;
  • Liu, Xiao-Long (Institute of Parasitic Diseases, School of Basic Medical Sciences and Forensic Medicine, Hangzhou Medical College) ;
  • Eamsobhana, Praphathip (Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University) ;
  • Gan, Xiao-Xian (Institute of Parasitic Diseases, School of Basic Medical Sciences and Forensic Medicine, Hangzhou Medical College)
  • 투고 : 2020.10.24
  • 심사 : 2021.02.23
  • 발행 : 2021.06.30
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초록

Human gnathostomiasis is a parasitic disease caused by Gnathostoma nematode infection. A rapid, reliable, and practical immunoassay, named dot immuno-gold filtration assay (DIGFA), was developed to supporting clinical diagnosis of gnathostomiasis. The practical tool detected anti-Gnathostoma-specific IgG4 in human serum using crude extract of third-stage larvae as antigen. The result of the test was shown by anti-human IgG4 monoclonal antibody conjugated colloidal gold. The sensitivity and specificity of the test were both 100% for detection in human sera from patients with gnathostomiasis (13/13) and from healthy negative controls (50/50), respectively. Cross-reactivity with heterogonous serum samples from patients with other helminthiases ranged from 0 (trichinosis, paragonimiasis, clonorchiasis, schistosomiasis, and cysticercosis) to 25.0% (sparganosis), with an average of 6.3% (7/112). Moreover, specific IgG4 antibodies diminished at 6 months after treatment. This study showed that DIGFA for the detection of specific IgG4 in human sera could be a promising tool for the diagnosis of gnathostomiasis and useful for evaluating therapeutic effects.

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과제정보

The authors would like to thank the Director of Zhejiang Academy of Medical Sciences for permission to conduct the present study. We thank Drs. Ganyu Xu and Kokouvi Kassegne for improving English-language presentation of this paper. This work was financially supported by the Medical Science & Technology Project of Zhejiang Province (grant no. 2011 KY001), the Foundation of National Science and Technology Major Program (grant no. 2012ZX10004-220) and the Open Project of Key Laboratory of Parasite and Vector Biology, Ministry of Health (grant no. WSBKTKT201501).

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