Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Assessment of the ozonation against pathogenic bacteria in the effluent of the quarantine station

  • Park, Seon Yeong (Program in Environmental and Polymer Engineering, INHA University) ;
  • Kim, Joo Han (Department of Environmental Engineering, INHA University) ;
  • Kim, Chang Gyun (Program in Environmental and Polymer Engineering, INHA University)
  • Received : 2021.03.15
  • Accepted : 2021.06.08
  • Published : 2021.06.30
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Abstract

This study investigated how ozone treatment can successfully inactivate pathogenic bacteria in both artificial seawater and effluents discharged from the fishery quarantine station in Pyeongtaek Port, Korea. Vibrio sp. and Streptococcus sp. were initially inoculated into the artificial seawater. All microbes were almost completely inactivated within 10 min and 30 min by injecting 6.4 mg/min and 2.0 mg/min of ozone, respectively. It was discovered that the water storing Pleuronichthys, Pelteobagrus, and Cyprinus imported from China contained the indicator bacteria, Vibrio sp., Enterococcus sp., total coliforms, and heterotrophic microorganisms. Compared to the control, three indicator bacteria were detected at two to six times higher concentrations. The water samples displayed a diverse microbial community, comprising the following four phyla: Bacteroidetes, Proteobacteria, Firmicutes, and Actinobacteria. Almost all indicator bacteria were inactivated in 5 min at 2.0 mg/min of ozonation; comparatively, 92.9%-98.2% of the less heterotrophic microorganisms were deactivated within the same time period. By increasing the dosage to 6.4 mg/min, 100% deactivation was achieved after 10 min. Despite the almost complete inactivation of most indicator bacteria at high doses after 10 min, several bacterial strains belonging to the Proteobacteria have still been found to be resistant under the given operational conditions.

Keywords

Acknowledgement

This research was part of a project entitled "Development of a water treatment system to remove harmful substances of ecological disturbances emitted from quarantine stations screening up imported fishery products" (No. 20180341) supported by the Korea Institute of Marine Science and Technology Promotion. It was also partially supported by INHA University Grant. In addition, the employed marine bacteria for the disinfection test were provided by the Korean Culture collection of Aquatic Microorganisms (KoCAM) under the National Institute of Fisheries Science.

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