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Hepatoprotective effect of fermented Chrysanthemum indicum L. water extract on ethanol-induced liver injury in HepG2 cells

감국 발효 열수 추출물의 에탄올에 의해 손상된 HepG2 세포의 간보호 효과

  • Seo, Tae-Su (R&D Center, Sungyibio) ;
  • Han, Joon-Hee (Department of Research and Development, Chuncheon Bioindustry Foundation) ;
  • Hong, Min (Department of Research and Development, Chuncheon Bioindustry Foundation) ;
  • Choi, Da-Hye (Department of Research and Development, Chuncheon Bioindustry Foundation) ;
  • Lee, Deug-Chan (Department of Medical Biotechnology School of Biomedical Science Kangwon National University) ;
  • Yu, Keun-Hyung (Food&Drug Business Headquarters, Adbiotech)
  • 서태수 (성이바이오(주) 기업부설연구소) ;
  • 한준희 ((재)춘천바이오산업진흥원 기술개발실) ;
  • 홍민 ((재)춘천바이오산업진흥원 기술개발실) ;
  • 최다혜 ((재)춘천바이오산업진흥원 기술개발실) ;
  • 이득찬 (강원대학교 의생명과학대학 의생명공학과) ;
  • 유근형 ((주)애드바이오텍 식의약사업본부)
  • Received : 2020.12.07
  • Accepted : 2021.04.29
  • Published : 2021.06.30

Abstract

This study aimed to identify the hepatoprotective effects of a fermented Chrysanthemum indicum L. water extract. The extraction yield, antioxidant activities (ABTS and DPPH), and content of luteolin and luteolin-7-glucoside were significantly higher in the fermented C. indicum L. water extract (FCI) than in the C. indicum L. water extract. Treatment with FCI (200 ㎍/mL) significantly reduced the activities of gamma(γ)-glutamyl transferase, aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase in HepG2 cells treated with 3% ethanol. Furthermore, FCI did not affect the viability of the HepG2 cells. These results suggest that FCI can be useful for the development of an effective hepatoprotective agent.

본 연구에서는 발효한 감국 발효 열수 추출물의 luteolin 및 luteolin-7-glucoside 함량, 항산화 활성 및 간세포 보호효과를 비교 분석함으로써 발효에 의한 활성차이를 분석하였다. 감국 내 luteolin 및 luteolin-7-glucoside 함량은 발효 전과 비교하여 유의하게 증가하였고 총 폴리페놀 함량 역시 발효 전보다 증가하는 것으로 나타났다. 유산균 발효에 의해 ABTS radical 소거능은 추출물의 농도가 50, 100, 200 및 400 ㎍/mL의 범위에서 7.30, 15.55, 28.30, 44.48%로 감국 열수 추출물과 비교하여 증가하는 것으로 나타났다. DPPH radical 소거능 역시 ABTS radical 소거능을 비교한 결과와 유사하게 같은 농도의 범위에서 감국 발효 열수 추출물의 경우 각각 6.03, 12.25, 26.18, 41.05%로 감국 열수 추출물과 비교하여 증가하는 것으로 나타났다. HepG2 세포에 감국 열수 추출물과 감국 발효 열수 추출물을 처리한 후 48시간 배양한 결과 모든 처리구에서 세포독성이 없는 것으로 나타났다. HepG2 세포에 에탄올을 처리하여 간독성을 유발한 결과 에탄올 처리한 모든 처리구에서 GGT, AST, ALT 및 LDH 활성이 증가하였고, 감국 발효 열수 추출물을 100 ㎍/mL을 처리하였을 때 대조구과 비교하여 GGT, AST 및 ALT 활성이 유의적으로 감소하는 것으로 나타났으며, LDH 활성은 감국 발효 열수 추출물을 200 ㎍/mL을 처리하였을 때 대조구과 비교하여 감소하는 것으로 나타났다. 이상의 결과로 볼 때 감국 발효 열수 추출물은 luteolin 및 luteolin-7-glucoside 함량 증가, 항산화 활성 증가 및 GGT, AST, ALT, LDH의 활성을 감소시킴으로써 에탄올에 의한 간 손상을 예방하는 것으로 사료되며, 동물실험을 통한 추가적인 간 기능개선 검증이 필요하다고 판단된다.

Keywords

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