Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Antimicrobial Resistance Profile of Acinetobacter spp. Isolates from Retail Meat Samples under Campylobacter-Selective Conditions

  • Cha, Min-Hyeok (Laboratory of Food Safety and Evaluation, Department of Biotechnology, Korea University Graduate School) ;
  • Kim, Sun Hee (Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Seokhwan (Division of Food Microbiology, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Lee, Woojung (Division of Food Microbiology, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Kwak, Hyo-Sun (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Chi, Young-Min (Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University) ;
  • Woo, Gun-Jo (Laboratory of Food Safety and Evaluation, Department of Biotechnology, Korea University Graduate School)
  • Received : 2021.02.22
  • Accepted : 2021.04.02
  • Published : 2021.05.28
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Abstract

Acinetobacter strains are widely present in the environment. Some antimicrobial-resistant strains of this genus have been implicated in infections acquired in hospitals. Genetic similarities have been reported between Acinetobacter strains in nosocomial infections and those isolated from foods. However, the antimicrobial resistance of Acinetobacter strains in foods, such as meat, remains unclear. This study initially aimed to isolate Campylobacter strains; instead, strains of the genus Acinetobacter were isolated from meat products, and their antimicrobial resistance was investigated. In total, 58 Acinetobacter strains were isolated from 381 meat samples. Of these, 32 strains (38.6%) were from beef, 22 (26.5%) from pork, and 4 (4.8%) from duck meat. Antimicrobial susceptibility tests revealed that 12 strains were resistant to more than one antimicrobial agent, whereas two strains were multidrug-resistant; both strains were resistant to colistin. Cephalosporin antimicrobials showed high minimal inhibitory concentration against Acinetobacter strains. Resfinder analysis showed that one colistin-resistant strain carried mcr-4.3; this plasmid type was not confirmed, even when analyzed with PlasmidFinder. Analysis of the contig harboring mcr-4.3 using BLAST confirmed that this contig was related to mcr-4.3 of Acinetobacter baumannii. The increase in antimicrobial resistance in food production environments increases the resistance rate of Acinetobacter strains present in meat, inhibits the isolation of Campylobacter strains, and acts as a medium for the transmission of antimicrobial resistance in the environment. Therefore, further investigations are warranted to prevent the spread of antimicrobial resistance in food products.

Keywords

Acknowledgement

This work was supported by a grant from the Ministry of Food and Drug Safety (nos. 15161MFDS645), the Korea National Institute of Health (grant number 2017-NI41004 and 2017ER540601), and the Korea Rural Development Administration (grant number PJ010500). The findings and conclusions of this article are ours and do not necessarily reflect the views of MFDS, NIH, and KRDA.

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