Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Effects of exogenous hormones treatment on spermiation and plasma levels of gonadal steroids in Roughscale sole, Clidoderma asperrimum

  • Woo, Sol Min (Department of Marine and Fisheries Resources, Mokpo National University) ;
  • Lee, Hyo Bin (Department of Biomedicine, Health & Life Convergence Sciences, BK21, Mokpo National University) ;
  • Seo, Young Seok (Gyeongsangbuk-do Fisheries Resources Institute) ;
  • Lim, Han Kyu (Department of Biomedicine, Health & Life Convergence Sciences, BK21, Mokpo National University)
  • Received : 2021.08.06
  • Accepted : 2021.11.29
  • Published : 2021.12.31
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Abstract

Roughscale sole (Clidoderma asperrimum) is only wild caught because basic reproductive research on this species is lacking and gamete production in an artificial setting has not been successful. Exogenous hormone treatment has been used to induce gonadal maturation and final spermiation in wild-caught individuals. In this study, the effects of an exogenous hormone on spermiation in roughscale sole was investigated by implanting different concentrations of a salmon gonadotrophin-releasing hormone analog (sGnRH; 0, 25, 50, and 100 ㎍/kg body weight) into male fishes. The control group did not produce sperm after 21 days post-implantation, and the duration of spermiation was shorter compared to the other groups. The spermiation period and milt amount differed among the hormone-treated groups according to the hormone concentration used. Milt volumes in the groups treated with 25 and 100 ㎍/kg sGnRH increased compared to the control group, whereas exogenous hormone treatment had no effect on the movable sperm ratio. The spermatocrit was high at the beginning of spermiation in all groups and then tended to decrease gradually over time except in the experimental group treated with 100 ㎍/kg sGnRH. Plasma levels of testosterone, 11-ketotestosterone, 17α, 20β-dihydroxy-4-pregnen-3-one were not significantly affected by the sGnRH treatments. Our results demonstrate that it is possible to prolong the spermiation period and increase milt volume by treating male roughscale soles with an exogenous hormone. In addition, the artificial hormone treatment did not affect sperm motility.

Keywords

Acknowledgement

This study was supported by the Korea Institute of Marine Science & Technology Promotion, Korea (Assignment No. 20180373).

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