Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Identification of the bacterial composition in the rockworm gut and biofloc-fed adult gut flora beneficial for integrated multitrophic aquaculture

  • Jung, Hyun Yi (Department of Biotechnology, Pukyong National University) ;
  • Kim, Chang Hoon (Department of Marine Bio-Materials and Aquaculture, Pukyong National University) ;
  • Kim, Joong Kyun (Department of Biotechnology, Pukyong National University)
  • Received : 2021.07.07
  • Accepted : 2021.08.08
  • Published : 2021.09.30
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Abstract

The rockworm gut flora was identified at each growth stage to elucidate the composition of the bacterial community. The source material was Marphysa sanguinea fed regular feed and fed biofloc at the adult stage in parallel. The systematic bacterial community composition was determined based on the next-generation sequencing method, and alpha diversity and beta diversity were conducted to access the species diversity within and between the bacterial communities, respectively. The composition of the gut flora changed considerably as the rockworms developed. The shift in the gut flora was confirmed at the phylum, family, and genera level of the bacterial communities. The Vibrio species associated with high rockworm mortality occupied 7.7% of the gut flora at the larval stage; however, they disappeared in the healthy adult gut. Moreover, different gut flora was observed between adults fed regular feed and those fed biofloc. Specifically in the biofloc-fed adult gut, several immune relevant and water-purifying bacteria were detected. The biofloc-fed adult gut flora could decompose and mineralize organic sediment, and thus be effectively utilized for integrated multitrophic aquaculture. The Venn diagram revealed that only two bacterial species were shared throughout all growth stages, and the biofloc-fed adults exhibited the highest diversity within the bacterial community.

Keywords

Acknowledgement

This work was supported by a Research Grant of Pukyong National University (Year 2019).

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