The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Identification of Plant Viruses Infecting Pear Using RNA Sequencing

  • Kim, Nam-Yeon (Department of Applied Biology, Institute of Environmentally Friendly Agriculture, Chonnam National University) ;
  • Lee, Hyo-Jeong (Department of Applied Biology, Institute of Environmentally Friendly Agriculture, Chonnam National University) ;
  • Kim, Hong-Sup (Seed Testing & Research Center, Korea Seed & Variety Service) ;
  • Lee, Su-Heon (School of Applied Biosciences, Kyungpook National University) ;
  • Moon, Jae-Sun (Plant Genome Research Center, Korea Research Institute of Biosciences & Biotechnology) ;
  • Jeong, Rae-Dong (Department of Applied Biology, Institute of Environmentally Friendly Agriculture, Chonnam National University)
  • Received : 2021.01.25
  • Accepted : 2021.04.23
  • Published : 2021.06.01
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Abstract

Asian pear (Pyrus pyrifolia) is a widely cultivated and commercially important fruit crop, which is occasionally subject to severe economic losses due to latent viral infections. Thus, the aim of the present study was to examine and provide a comprehensive overview of virus populations infecting a major pear cultivar ('Singo') in Korea. From June 2017 to October 2019, leaf samples (n = 110) of pear trees from 35 orchards in five major pear-producing regions were collected and subjected to RNA sequencing. Most virus-associated contigs matched the sequences of known viruses, including apple stem grooving virus (ASGV) and apple stem pitting virus (ASPV). However, some contigs matched the sequences of apple green crinkle-associated virus and cucumber mosaic virus. In addition, three complete or nearly complete genomes were constructed based on transcriptome data and subjected to phylogenetic analyses. Based on the number of virus-associated reads, ASGV and ASPV were identified as the dominant viruses of 'Singo.' The present study describes the virome of a major pear cultivar in Korea, and looks into the diversity of viral communities in this cultivar. This study can provide valuable information on the complexity of genetic variability of viruses infecting pear trees.

Keywords

Acknowledgement

This work was supported by Korea institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through (Agri-Bioindustry Technology Development Program), funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (No. 317006-04-2-HD030).

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