대한본초학회지 (The Korea Journal of Herbology)

  • 제36권3호
  • /
  • Pages.47-53
  • /
  • 2021
  • /
  • 1229-1765(pISSN)
  • /
  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
DOI QR코드

DOI QR Code

의이인의 정유 분획물이 모유두 세포의 성장에 미치는 영향

Effect of essential oil from Coicis Semen (ECS) on proliferation of human hair dermal papilla cells

  • 김유진 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 서경혜 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 장귀영 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 정지욱 (대구한의대학교 바이오산업대학 바이오산업융합학부) ;
  • 김미려 (대구한의대학교 한의과대학 본초약리학교실)
  • Kim, Yoo-Jin (Department of Herbal Pharmacology, College of Korean Medicine, Daegu Haany University) ;
  • Seo, Kyung Hye (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Jang, Gwi Young (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Jung, Ji Wook (Division of Bio-technology and Convergence, College of Herbal Bio-Industry, Daegu Haany University) ;
  • Kim, Mi Ryeo (Department of Herbal Pharmacology, College of Korean Medicine, Daegu Haany University)
  • 투고 : 2021.04.12
  • 심사 : 2021.05.25
  • 발행 : 2021.05.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives : Currently, the alopecia is one of the most emotionally stressful syndromes in human life. Human hair dermal papilla cells (HDPCs) play an essential role in controlling hair growth and in regulating hair cycle. We performed MTT assay, cell cycle, and western blot to determine the effects of essential oil from Coicis Semen (ECS) on hair growth in HDPCs. Methods : We monitored cell proliferations by MTT assay in HDPCs. After setting up the safe and effective concentration range to be treated ECS, cell cycle analysis was performed using flow cytometry. Also, the protein expression of hair growth-related factors such as insulin like growth factor-1 (IGF-1), Wnt, extracellular signal-regulated kinase (ERK), serine/threonine-specific protein kinase (Akt) in HDPCs was determined by western blot. Results : As results, cell proliferation was increased in ECS group compared to dimethyl sulfoxide (DMSO) group and minoxidil (MNXD) group. Cell number of ECS group was more decrease in sub G1 phase than cell number of DMSO group. Also, cell number of ECS group increased compared to cell number of DMSO group in G1 phase. Protein expression of ECS group was higher than protein expression of DMSO group on related hair growth factors (IGF-1, Wnt, ERK, Akt). Conclusion : As mentioned above, ECS increased cell proliferation and the protein expression of IGF-1, Wnt, ERK, and Akt. These results suggest that ECS could be used as a potential material for the treatment of alopecia by increasing the proliferation of HDPCs.

키워드

과제정보

본 성과물은 농촌진흥청 연구사업(세부과제번호: PJ01389501)의 지원에 의해 연구되었으며, 이에 감사드립니다.

참고문헌

  1. Price VH. Treatment of hair loss. N Engl J Med. 1999 ; 341 : 964-73. https://doi.org/10.1056/NEJM199909233411307
  2. Ellis JA, Sinclair R, Harrap SB. Androgenetic alopecia: pathogenesis and potential for therapy. Expert Rev Mol Med. 2002 ; 4 : 1-11.
  3. Heo J. Dongeuibogam. Seoul: Koryeomoonwhasa. 1997 : 307-10.
  4. Headington JT. Histological findings in androgenic alopecia treated with topical minoxidil. Br J Dermatol. 1982 ; 107(22) : 20-1.
  5. Do EJ, Hwang MY, Kim SY, Lee JS, Yang DS, Yang CH, Kim MR. The effect of Gyungokgo-gamibang extract on hair growth and protein expression in mice. Kor J Herbol. 2011 ; 26(4):9-14. https://doi.org/10.6116/KJH.2011.26.4.009
  6. Buhl AE, Waldon DJ, Conrad SJ, Mulholland MJ, Kubicek MF, Johnson GA, Brunden MN, Stefanski KJ, Stehle RG, Gadwood RC, Kamdar BV, Thomasco LM, Schostarez HJ, Schwartz TM, Diani AR. Potassium channel conductance: a mechanism affecting hair growth both in vitro and in vivo. J Invest Dermatol. 1992 ; 98(3) : 315-9. https://doi.org/10.1111/1523-1747.ep12499788
  7. Han JH, Kwon OS, Chung JH, Cho KH, Eun HC, Kim KH. Effect of minoxidil on proliferation and apoptosis in dermal papilla cells of human hair follicle. J Dermatol Sci. 2004 ; 34(2) : 91-8. https://doi.org/10.1016/j.jdermsci.2004.01.002
  8. Meisheri KD, Cipkus LA, Taylor CJ. Mechanism of action of minoxidil sulfate-induced vasodilation: a role for increased K+ permeability. J Pharmacol. 1988 ; 245(3) ; 751-60.
  9. Melcangi RC, Caruso D, Abbiati F, Giatti S, Calabrese D, Piazza F, Cavaletti G. Neuroactive Steroid Levels are Modified in Cerebrospinal Fluid and Plasma of Post-Finasteride Patients Showing Persistent Sexual Side Effects and Anxious/Depressive Symptomatology. J Sexual Medicine. 2013 ; 10(10) : 2598-603. https://doi.org/10.1111/jsm.12269
  10. Hagemann T, Schlutter-Bohmer B, Allam JP, Bieber T, Novak N. Positive lymphocyte transformation test in a patient with allergic contact dermatitis of the scalp after short-term use of topical minoxidil solution. Cont Derm. 2005 ; 53(1) : 85-97.
  11. Kwack MH, Ahn JS, Kim MK, Kim JC, Sung YK. Preventable effect of L-threonate, an ascorbate metabolite, on androgen-driven balding via repression of dihydrotestosterone induced dickkopf-1 expression in human hair dermal papilla cells. BMB Reports. 2010 ; 43(10) : 688-92. https://doi.org/10.5483/BMBRep.2010.43.10.688
  12. Hardy MH. The secret life of the hair follicle. Trends Genet. 1992 ; 8(2) : 55-61. https://doi.org/10.1016/0168-9525(92)90350-D
  13. Cotsarelis G. Epithelial stem cells: a folliculocentric view. J Invest Dermatol. 2006 ; 126(7) : 1459-68. https://doi.org/10.1038/sj.jid.5700376
  14. Millar SE. Molecular mechanisms regulating hair follicle development. J Invest Dermatol. 2002 ; 118(2) : 216-25. https://doi.org/10.1046/j.0022-202x.2001.01670.x
  15. Kim MJ, Yang BH and Kim SM. Effect of fragrance of weeds on the behavior of consumers. Weed & Turfgrass Sci. 2018 ; 7 : 98-110.
  16. Hyung SH, Gang SR, Kim YC. Effect of complex oriental medicine extract on hair growth promotion in an alopecia model of C57BL/6 mice. J Korean Soci Cosmetol. 2007 ; 3 : 1366-75.
  17. Cho SI, Kim HW, Kim JS. Effect of Coicis semen extract on streptozotocininduced diabetic nephropathy rats. Kor J Herbol. 2006 ; 21 : 75-81.
  18. Lee YJ, Sohn YJ, Lee ES, Park JS, Kim SK. Effects of Coicis semen on the hyperlipidemia in rat. Kor J Herbol. 2004 ; 19 : 129-36.
  19. Yun HJ, Lee YJ, Kang MS, Baek JH. Inhibitory effect of Coicis semen extract (CSE) on pro-inflammatory mediatory. J Korean Orient Pediatr. 2009 ; 23 : 159-71.
  20. Hung WC, Chang HC. Methanolic extract of adlay seed suppresses COX-2 expression of human lung cancer cells via inhibition of gene transcription. J Agric Food Chem. 2003 ; 51 : 7333-7. https://doi.org/10.1021/jf0340512
  21. Lu X, Liu W, Wu J, Li M, Wang J, et al. A polysaccharide fraction of adlay seed (Coixlachryma-jobi L.) induces apoptosis in human non-small cell lung cancer A549 cells. Biochem Biophys Res Commun. 2013 ; 430 : 846-51. https://doi.org/10.1016/j.bbrc.2012.11.058
  22. Lee SI. Herbal medicine. Hong Lim Sa. 1991 ; 306-8.
  23. Jung MJ. Effects of Coicis Semen Water Extract on Hair Growth in Alopecia Models. Dongeui University Master's degree. 2017.
  24. Kim YJ, Lee YJ, Kim MR. Effect of herbal mixture (RAA) on hair growth in C57BL/6 mice. Kor J Herbol. 2019 ; 34(6) : 139-46. https://doi.org/10.6116/KJH.2019.34.6.139
  25. Kim JY, Kim YJ, Kim MJ, Kim MR. Ethanol extract of medicinal herbal mixture accelerates hair growth and melanogenesis in vivo and in vitro. Kor J Herbol. 2018 ; 33(5) : 9-18. https://doi.org/10.6116/kjh.2018.33.5.9
  26. Kim JD. Literature on the quality and effect of Job's Tears (Coix lachryma -jobi L. var. mayuen S.). Korea Agric Hist Assoc. 2012 ; 11(1) : 89-122.
  27. Ukita T, Tanimura A. Studies on the anti-tumor component in the seeds of Coix lachrymal-jobi L. var. ma-yuen Stapf, II. Chem Phar Bul. 1961 ; l9 : 47-52. https://doi.org/10.1248/cpb.9.47
  28. Numata M, Yamamoto A, Moribayashi A, Yamada H. Antitumor components isolated from the Chinese herbal medicine Coix lachryma-jobi. Planta Med. 1994 ; 60 : 356-9. https://doi.org/10.1055/s-2006-959500
  29. Takahashi M, Konno C, Hikino H. Isolation and hypoglycemic activity of coixans A, B and C, glycans of Coix lachryma-jobi var. mayuen seeds. Planta Med. 1986 ; 64-5.
  30. Lee JY, Park JY, Kim DH, Choi SJ, Jang GY, Seo KH. Chemical composition and protective effect of essential oils derived from medicinal plant on PC12 neuro-cells induced by oxidative stress. Korean J Food Nutr. 2020 ; 33(2) : 215-21. https://doi.org/10.9799/KSFAN.2020.33.2.215
  31. Ha BJ. Aromatherapy. Soomoonsa. Gyeonggi. 2000 ; 11-25.
  32. Ahn KM. The effect of aroma inhalation method using sweet orange essential oil on stress. J Kor Soc Cosm. 2007 ; 13 : 1121-9.
  33. Seo HK, Park KS. A study on the effects of aroma inhalation method using clarysage essential oil on stress in middle-aged women. Kor J Women Health Nurs. 2003 ; 9 : 61-9. https://doi.org/10.4069/kjwhn.2003.9.1.61
  34. Jeong SH, Park JA. The effect of parallel therapy with abdominal massage using aroma and chiropractic on the pelvis and body fat. Kor J Aesthet Cosmetol. 2012 ; 10 : 381-7.
  35. Lee WJ, Hong SG. A review of aromatherapy on stress management in sportsman. Kor J Aesthet Cosmetol. 2010 ; 8 : 412-28.
  36. Cheong KJ, Yun MY, Chin CH, Song HH, Lee SS, Cheong KJ. A study on the effects of aroma blending oil(carrot seed, geranium, lemon, jojoba) on atopy dermatitis. Kor J Aesthet Cosmetol. 2010 ; 8 : 287-97.
  37. Park JS. Effect of Origanum vulgare Extracts on Hair regeneration. Kor J Pharmacogn. 2013 ; 43(3) : 275-80.
  38. Kim JR, Kim MJ, Kim YJ, Yoon YM, An SK. Analysis of the Gens Expression Profiling in Human Dermal Papilla Cells Stimulated by Hydrogen peroxide. J Kor Soc Cosm. 2012 ; 18(1) : 110-9.
  39. Lee YK, Choi JH. Effects of Black Soybean and Germinated Black Soybean Extracts on proliferation of primary human follicle dermal papilla cells. J Kor Soci Well. 2015 ; 10(1) : 293-301.
  40. Shin HS, Park SY, Hwang ES, Lee DG, Mavlonov GT, Yi TH. Ginsenoside F2 Reduces Hair Loss by Controlling Apoptosis through the Sterol Regulatory Element-Binding Protein Cleavage Activating Protein and Transforming Growth Factor-β Pathways in a Dihydrotestosterone-Induced Mouse Model. Biol Pharm Bull. 2014 ; 37(5) : 755-63. https://doi.org/10.1248/bpb.b13-00771
  41. Lee JS, Kim YC. Regular Articles : The Promoting Effect of Angelica gigas Nakai and Glycyrrhiza uralensis Fischet Oriental Medicine Complex Extracts on Hair Growth. J Cosme Sci. 2010 ; 6(1) : 49-56.
  42. Itami S, Kurata S, Takayasu S. Androgen induction of follicular epithelial cell growth is mediated via insulin-like growth factor-1 from dermal papilla cells. Biochem Biophys Res Commun. 1995 ; 212 : 988-94. https://doi.org/10.1006/bbrc.1995.2067
  43. Philpott MP, Green MR. Human hair growth in vitro. J Cell. 1990 ; 97 : 463-71.
  44. Cai Y. Mohseny AB, Karperien M, Hogendoorn PC, Zhou G, Cleton-Jansen AM. Inactive Wnt/β-catenin pathway in conventional high-grade osteosarcoma. J Patholo. 2010 ; 220(1) : 24-33. https://doi.org/10.1002/path.2628
  45. Wood LS. New therapeutic strategies for renal cell carcinoma. Urologic Nursing. 2010 ; 30 : 40-53. https://doi.org/10.7257/1053-816X.2010.30.1.40
  46. Han JH, Kwon OS, Chung JH, Cho KH, Eun HC, Kim KH. Effect of minoxidil on proliferation and apoptosis in dermal papilla cells of human hair follicle. J Dermatol Sci. 2004 ; 34(2) : 91-8. https://doi.org/10.1016/j.jdermsci.2004.01.002