DOI QR코드

DOI QR Code

인돌의 항균 효과에 의한 Pantoea agglomerans의 바이오필름 생성 억제 조절에 관한 연구

Study on the Control of Biofilm Formation Inhibition on Pantoea agglomerans by Anti-bacterial Effect of Indole

  • 진슬 (한동대학교 생명과학부) ;
  • 양웅석 (포항노다지마을(주)) ;
  • 황철원 (한동대학교 GLS학부) ;
  • 이재용 (경운대학교 항공신소재공학과)
  • Jin, Seul (Department of Life-Science, Handong Global University) ;
  • Yang, Woong-Suk (Nodaji R&D Center, Nodaji Co., Ltd.) ;
  • Hwang, Cher-Won (Global Leadership School, Handong Global University) ;
  • Lee, Jae-Yong (Department of Advanced Aerospace Materials Engineering, Kyungwoon University)
  • 투고 : 2021.01.18
  • 심사 : 2021.04.19
  • 발행 : 2021.05.31

초록

In this study, we investigated the effects of indole on biofilm formation inhibition in Pantoea agglomerans (P. agglomerans). In the biofilm growth assay, indole inhibited biofilm formation across all the growth time. Depending on biofilm growth stage, indole exhibited biofilm inhibition and anti-bacterial effects on planktonic cells. Through the analysis of the proportion rate between biofilm and Colony Forming Units (CFU) and inhibition rate of indole, we confirmed that depending on the biofilm stage of P. agglomerans, indole treatment timing was more important than the treatment duration. By comparing gene expression rates through rt-qPCR P.agglomerans affected by indole was found to significantly change quorum sensing (pagI/R) and indole transportation (bssS) gene expressions. Throughout all, indole exhibited both antimicrobial and anti-biofilm effects on P. agglomerans. In addition, we confirmed the anti-biofilm effects of indole on mature biofilm. In conclusion, indole as a signal molecule, can exhibit anti-biofilm effects through bacterial quorum sensing inhibition and indole affects. Therefore, indole can regulate biofilm bacteria especially gram-negative opportunistic pathogens.

키워드

과제정보

본 논문은 2019년도 정부(교육부)의 제원으로 한국연구재단의 지원을 받아 수행된 기초연구 사업(2017R1D1A1B03032867)입니다.

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