Journal of the Korean Society of Marine Environment & Safety (해양환경안전학회지)

The Korean Society of Marine Environment and safety (해양환경안전학회)
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Effects of Physicochemical and Environmental Factors on Spatial and Temporal Variations in Phytoplankton Pigment and its Community Composition in Jinhae Bay

진해만에서 물리화학적 환경요인이 식물플랑크톤 색소 및 군집조성의 시공간적 변화에 미치는 영향

  • Na, Sujin (Marine Environment Research Division, National Institute of Fisheries Science) ;
  • Lee, Jiyoung (Marine Environment Research Division, National Institute of Fisheries Science) ;
  • Kim, Jeong Bae (Marine Environment Research Division, National Institute of Fisheries Science) ;
  • Koo, Jun-Ho (Marine Environment Research Division, National Institute of Fisheries Science) ;
  • Lee, Garam (Marine Environment Research Division, National Institute of Fisheries Science) ;
  • Hwang, Hyunjin (Marine Environment Research Division, National Institute of Fisheries Science)
  • 나수진 (국립수산과학원 어장환경과) ;
  • 이지영 (국립수산과학원 어장환경과) ;
  • 김정배 (국립수산과학원 어장환경과) ;
  • 구준호 (국립수산과학원 어장환경과) ;
  • 이가람 (국립수산과학원 어장환경과) ;
  • 황현진 (국립수산과학원 어장환경과)
  • Received : 2021.03.03
  • Accepted : 2021.04.27
  • Published : 2021.04.30
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Abstract

The aim of this study was to investigate the spatial and temporal distribution of phytoplankton biomass and community composition in Jinhae Bay on the southern coast of Korea. Phytoplankton pigment analysis was conducted using ultra performance liquid chromatography (UPLC) were conducted from April to December 2019 at seven stations. Temperature, salinity, and dissolved oxygen (DO) and inorganic nutrients (dissolved nitrogen, dissolved phosphorus, and orthosilicic acid) were measured to investigate the environmental factors associated with the structure of phytoplankton community. Phytoplankton biomass (Chl-a) was the highest in July (mean 15.4±4.3 ㎍/L) and the lowest in December (mean 3.5±0.6 ㎍/L). Fucoxanthin was the most abundant carotenoid and showed a similar variation pattern to Chl-a, peridinin, and Chl-b. Phytoplankton community composition analysis showed that diatoms were a predominant group with an average abundance of 70 % whereas chlorophytes, cryptophytes, and dinoflagellates often appeared with lower averages. Further, the dominance of diatoms was closely correlated with water temperature and N:P ratio, which might be influenced by high temperatures in the summer and nutrient loading from the land. Additionally, freshwater and nutrient input by rainfall was estimated to be the most important environmental factor. Hence, the spatial and temporal variations in the composition of phytoplankton pigments and phytoplankton community were correlated with physicochemical and environmental parameters.

본 연구에서는 ultra performance liquid chromatography(UPLC)를 이용하여 진해만의 식물플랑크톤 생체량 및 군집구조의 시공간적 분포에 미치는 환경요인의 영향을 조사하였다. 이를 위해 2019년 4월에서 12월까지 총 5회에 걸쳐 7개 정점에 대한 식물플랑크톤 색소분석과 수온, 염분, 용존산소(DO), 영양염(DIN, DIP, Si(OH)4) 등의 환경요인 분석을 행하였다. 조사기간 중 식물플랑크톤의 생체량(Chl-a)은 7월(평균 15.4±4.3 ㎍/L)에 가장 높았고, 12월(평균 3.5±0.6 ㎍/L)에 가장 낮았다. 보조색소의 경우 fucoxanthin이 가장 많이 검출되었고 그 다음으로 peridinin, Chl-b 순으로 나타났으며, 이들의 월 변동은 Chl-a와 유사한 경향을 보였다. 식물플랑크톤 군집분석결과, 규조류가 평균 70 %로 가장 우점하였으나, 일부 녹조류, 은편모조류, 와편모조류가 출현하기도 하였다. 우점종인 규조류는 특히 수온 및 N:P ratio와 밀접하게 연관되어 있어서 여름철 고온환경 및 육상으로부터의 영양염 유입에 민감하게 반응하는 것으로 추론되었다. 또한 식물플랑크톤 색소 및 종조성은 전반적으로 계절에 따른 물리화학적 환경요인의 변화 및 지형적 특성과 연관되어 있으며 강우로 인한 담수 및 영양염 공급에 큰 영향을 받는 것으로 추정되었다.

Keywords

Acknowledgement

이 논문은 2021년도 국립수산과학원 수산과학연구사업(R2021055)의 지원을 받아 수행된 연구입니다. 본 논문을 세심하게 검토해주신 심사위원분들께 감사드립니다.

References

  1. Anderson, R. A., R. R. Bidigare, M. D. Keller, and M. Latasa(1996), A comparison of HPLC pigment signatures and electron microscopic ovservations for oligotrophic waters of the North Atlantic and Pacific Oceans. Deep-Sea Research, Vol. 43, pp. 517-537. https://doi.org/10.1016/0967-0645(95)00095-X
  2. Bae, S. W., D. S. Kim, H. W. Choi, Y. O. Kim, C. H. Moon, and S. H. Baek(2014), The influence of nutrients addition on phytoplankton communities between spring and summer season in Gwangyang Bay, Korea. Journal of the Korean Society of Oceanography. Vol. 19, pp. 53-65.
  3. Baek, S. H., K. S. Shin, B. G. Hyun, P. G. Jang, H. S. Kim, and O. M. Hwang(2010), Distribution characteristics and community structure of phytoplankton in the different water masses during early summer of Southern Sea of Korea. Ocean and Polar Research, Vol. 32, pp. 1-13.
  4. Baek, S. H., S. J. Shimode, M. S. Han, and T. Kikuchi(2008), The influence of nutrients concentration and the ratio on phytoplankton community structure during late spring and early summer in Sagami Bay, Japan. Algae, Vol. 23, pp. 277-288. https://doi.org/10.4490/ALGAE.2008.23.4.277
  5. Baek, S. H. and Y. O. Kim(2010), The study of summer season in Jinhae Bay - Short-term changes of community structure and horizontal distribution characteristics of phytoplankton. Korean Journal of Environmental Biology, Vol. 28, pp. 115-124.
  6. Bidigare, R. P., T. J. Frank, C. Zastrow, and J. M. Brooks(1986), The distribution of algal chlorophylls and their degradation products in the Southern Ocean. Deep-Sea Research, Vol. 33, pp. 923-937. https://doi.org/10.1016/0198-0149(86)90007-5
  7. Bowles, N. D., H. W. Paerl, and J Tucker(1985), Effective solvents and extraction periods employed in phytoplankton carotenoid and chlorophyll determination. Fisheries and Aquatic Science, Vol. 42, pp. 1127-1131. https://doi.org/10.1139/f85-139
  8. Bray, J. R. and J. T. Curtis(1957), An ordination of upland forest communities of southern Wisconsin. Ecological Monographs, Vol. 27, pp. 325-349. https://doi.org/10.2307/1942268
  9. Brotas, V. and M. R. Plante-Cuny(1998), Spatial and temporal patterns of microphytobenthic taxa of estuarine tidal flats in the Tagus Estuary (Portugal) using pigment analysis by HPLC. Marine Ecology Progress Series, Vol. 171, pp. 43-57. https://doi.org/10.3354/meps171043
  10. Cartaxana, P., C. R. Mendes, M. A. van Leeuwe, and V. Bratas(2006), Comparative study on microphytobenthic pigments of muddy and sandy intertidal sediments of the Tagus estuary. Estuarine Coastal and Shelf Science, Vol. 66, pp. 225-230. https://doi.org/10.1016/j.ecss.2005.08.011
  11. Cho, K. J., M. Y. Choi, S. K. Kwak, S. H. Im, D. Y. Kim, J. G. Park, and Y. E. Kim(1998), Eutrophication and seasonal variation of Water Quality in Masan-Jinhae Bay. Journal of the Korean Society of Oceanography, Vol. 3, pp. 193-202.
  12. Cloern, J. E. and R. Dufford(2005), Phytoplankton community ecology: principles applied in San Francisco Bay. Marine Ecology Progress Series, Vol. 285, pp. 11-28. https://doi.org/10.3354/meps285011
  13. Fujiki, T., T. Toda, T. Kikuchi, H. Aono, and S. Taguchi(2004), Phosphorus limitation of primary productivity during the spring-summer blooms in Sagami Bay, Japan. Marine Ecology Progress Series, Vol. 283, pp. 29-38. https://doi.org/10.3354/meps283029
  14. Furuya, K., M. Hayasi, Y. Yabushita, and A. Lshikawa(2003), Phytoplankton dynamics in the East China Sea in spring and summer as revealed by HPLC-derived pigment signatures. Deep Sea Research. II, Vol. 50, pp. 367-387. https://doi.org/10.1016/S0967-0645(02)00460-5
  15. GNDI(2012), Gyung Nam Development Institute, Strategy for enhancement of shellfish farming in Gyungnam province, http://gndi.re.kr/gndi2016/report/sub01.html?mode=list&cate=&subcate=8,2012 (accessed 2012.02.14).
  16. Han, M. S., S. W. Kim, and Y. O. Kim(1991), Influence of discontinuous layer on plankton community structure and distribution in Masan Bay, Korea. Korean Journal of Fisheries and Aquatic Sciences, Vol. 24, pp. 459-471.
  17. Harvey, H. W.(1955), The chemistry and Fertility of sea waters. Cambridge University Press.
  18. Jang, P. K., W. J. Lee, M. C. Jang, J. D. Lee, W. J. Lee, M. Chang, K. C. Hwang, and K. S. Shin(2005), Spatial and temporal distribution of inorganic nutrients and factors controlling their distributions in Gwangyang Bay. Ocean and Polar Research, Vol. 28, pp. 359-379.
  19. Jin, S. H., S. H. Ryu, I. C. Lee, and K. H. Kim(2019), Estimation of oyster farming water quality index (OWQI) in Jinhae Bay, Korea. Journal of the Korean Society for Marine Environment & Energy, Vol. 22, pp. 246-252. https://doi.org/10.7846/JKOSMEE.2019.22.4.246
  20. Jung, M. H. and S. H. Yoon(2013), Temporal and spatial variability of phytoplankton communities in the Nakdong river estuary and coastal area, 2011-2012. Journal of the Korean Society of Oceanography, Vol. 18, pp. 214-226.
  21. Kang, C. K., Y. W. Lee, E. J. Choy, J. K. Shin, I. S. Seo, and J. S. Hong(2006), Microphytobenthos seasonality determines growth and reproduction in intertidal bivalves. Marine Ecology Progress Series, Vol. 315, pp. 113-127. https://doi.org/10.3354/meps315113
  22. Kilham, P. and R. E. Hecky(1988), Comparative ecology of marine and freshwater phytoplankton. Journal of Limnology and Oceanography, Vol. 33, pp. 776-795.
  23. Kim, D. S. and S. W. Kim(2003), Mechanism of oxygen-deficient water formation in Jindong Bay. Journal of Korean Society of Oceanography, Vol. 8, pp. 177-186.
  24. Kim, H. J., B. M. Kwak, J. H. Ahn, and J. S. Park(2014a), Simultaneous determination of Synephrine and N-Methyltyramine in orange fruit and juice from Korean market by UPLC-FLD. Korean Journal of Food Science and Technology, Vol. 46, pp. 276-282. https://doi.org/10.9721/KJFST.2014.46.3.276
  25. Kim, J. B., S. J. Hong, W. C. Lee, H. C. Kim, Y. W. Lee, S. H. Youn, and Y. S. Cho(2014b), Plankton community composition related to marine environmental factors in Haengam Bay. Journal of Environmental Science International, Vol. 23, pp. 2015-2028. https://doi.org/10.5322/JESI.2014.23.12.2015
  26. Kim, S. Y., Y. H. Lee, Y. S. Kim, J. H. Shim, J. J. Jeon, J. R. Hwang, and S. H. Jeon(2012), Characteristics of marine environmental in the hypoxic season at Jinhae Bay in 2010. Korean Journal of Nature Conservation, Vol. 6, pp. 115-129. https://doi.org/10.11624/KJNC.2012.6.2.115
  27. KMA(2019), Korea Meteorological Administation, Annual climatological report of 2019, p. 129.
  28. Lee, C. K., O. H. Lee, and S. G. Lee(2005), Impacts of temperature, salinity and irradiance on the growth of ten harmful algal bloom-forming microalgae isolated in Korean Coastal Waters. Journal of the Korean Society of Oceanography, Vol. 10, pp. 79-91.
  29. Lee, E. G., M. H. Son, J. B. Kim, W. C. Lee, G. E. Jeon, and S. H. Lee(2018), A study of variation characteristics of the phytoplankton community by UPLC located in the Jinju Bay, Korea. Korean Journal of Environmental Biology, Vol. 36, pp. 62-72. https://doi.org/10.11626/KJEB.2018.36.1.062
  30. Lee, J. H. and S. M. Youn(2000), Water quality and phytoplankton communities in Yeoja Bay of Korea. Algae, Vol. 15, pp. 89-98.
  31. Lee, J. Y.(2018), Hypoxia in Korean coastal waters: a case study of the natural Jinhae Bay and artificial Shihwa Bay. Frontiers in Marine Science, Vol. 69.1, pp. 1-24
  32. Lee, J. Y. and M. Jang(2014), Size dependent analysis of phytoplankton community structure during low water temperature periods in the coastal waters of East Sea, Korea. Korean Journal of Environmental Biology, Vol. 36, pp. 168-175.
  33. Lee, J. Y., S. G. Kim, and S. M. An(2017), Dynamics of the physical and biogeochemical processes during hypoxia in Jinhae Bay, South Korea. Journal of Coastal Research, Vol. 33.4, pp. 854-863.
  34. Lee, M. O., J. K. Kim, B. K. Kim, and M. W. Kim(2020), Past, present, and future directions in the study of Jinhae Bay, Korea, The Korean Society for Marine Environment & Energy, Vol. 23, pp. 57-69. https://doi.org/10.7846/JKOSMEE.2020.23.2.57
  35. Lee, Y. W., E. J. Choi, Y. S. Kim, and C. K. Kang(2009), Seasonal variations of microphytobenthos in sediments of the estuarine muddy sandflat of Gwangyang Bay: HPLC pigment analysis. Journal of the Korean Society of Oceanography, Vol. 14, pp. 48-55.
  36. Lee, Y. W. and G. Ki(2007), Linking groundwater-borne nutrients and dinoflagellate red-tide outbreaks in the southern sea of Korea using a Ra tracer. Journal of Estuarine, Vol. 71, pp. 309-317.
  37. Lee, Y. W., M. I. Park, J. H. Yoon, and S. B. Hur(2012), Temporal and spatial variation of microalgal biomass and community structure in seawater and surface sediment of the Gomso Bay as determined by chemotaxonomic analysis. Journal of the Korean Society of Oceanography, Vol. 17, pp. 87-94.
  38. Lee, Y. W., M. I. Park, Y. S. Kim, S. S. Kim, and C. K. Kang(2011), Application of photosynthetic analysis using a HPLC and CHEMTAX program to studies of phytoplankton community composition. Journal of Korean Society of Oceanography, Vol. 16, pp. 117-124.
  39. Legendre, P. and M. J. Anderson(1999), Distance based redundancy analysis: testing multispecies responses in multifactorial ecological experiments. Ecological monographs, Vol. 69.1, pp. 1-24. https://doi.org/10.1890/0012-9615(1999)069[0001:DBRATM]2.0.CO;2
  40. Macintyre, H. L., R. J. Geider, and D. C. Miller(1996), Microphytobenthos: The Ecological Role of the "Secret Garden" of Unvegetated, Shallow-Water Marine Habitats. I. Distribution, Abundance and Primary Production. Estuaries, Vol. 19, pp. 186-201. https://doi.org/10.2307/1352224
  41. Mackey, M. D., D. J. Mackey, H. W. Higgins, and S. W. Wright(1996), CHEMTAX - a program for estimating class abundances from chemical markers: application to HPLC measurements of phytoplankton. Marine Ecology Progress Series, Vol. 144, pp. 265-283. https://doi.org/10.3354/meps144265
  42. Mantoura, R. F. C. and C. A. Llewllyn(1983), The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by Reverse-Phase High-Performance Liquid Chromatography. Analytica Chimica Acta, Vol. 151, pp. 297-314. https://doi.org/10.1016/S0003-2670(00)80092-6
  43. NIFS(2020), National Institute of Fisheries Science, Annual monitoring report of marine environment around aquaculture area in Korea.
  44. Nybakken, J. W.(1993), Marine biology: an ecological approach. New York: Harper Collins College publishers.
  45. Oh, S. J., C. H. Moon, and M. O. Park(2004), HPLC analysis of biomass and community composition of microphytobenthos in the Saemankeum tidal flat, West coast of Korea. Korean Journal of Fisheries and Aquatic Sciences, Vol. 37, pp. 215-225. https://doi.org/10.5657/KFAS.2004.37.3.215
  46. Oh, S. J., J. S. Lee, J. S. Park, I. H. Noh, and Y. H. Yoon(2008), Environmental factor on the succession of phytoplankton community in Jinju Bay, Korea, Journal of Environmental Engineering, Vol. 11, pp. 98-104.
  47. Park, J. G., S. H. Huh, and H. J. Jeong(2001), Phytoplankton in Chinhae Bay: 1. Photosynthetic properties and primary production in variant light environments. Journal of Algae, Vol. 16, No. 2, pp. 189-196.
  48. Park, J. S.(1982), The characteristics of marine environment and red tide in Jinhae Bay. Annual Report of National Fisheries Research & Development, Vol. 28, pp. 55-88.
  49. Park, K. W., Y. S. Suh, and W. A. Lim(2012), Seasonal changes in phytoplankton composition in Jinhae Bay, 2011. Journal of the Korean Society of Marine Environment & Safety, Vol. 18, pp. 520-529. https://doi.org/10.7837/kosomes.2012.18.6.520
  50. Park, M. O. and J. S. Park(1997), HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton. Ocean Science Journal, Vol. 32, pp. 46-55.
  51. Sin, Y. S., B. K. Hyun, B. K. Jeong, and H. Y. Soh(2013), Impacts of eutrophic freshwater inputs on water quality and phytoplankton size structure in a temperate estuary altered by a sea dike. Marine Environmental research, 85, pp. 54-63. https://doi.org/10.1016/j.marenvres.2013.01.001
  52. Smith, D. E., M. Leffler, and G. Mackiernan(1992), Oxygen dynamics in the Chesapeake Bay: a synthesis of recent research. Maryland Sea Grant College, Maryland, p. 234.
  53. Son, M. H., D. S. Kim, and S. H. Beak(2014), Distributional characteristics of phytoplankton and nutrient limitation during spring season in Jinhae Bay. Korea Institute of OceanScience & Technology, Vol. 15, pp. 3345-3350.
  54. Trees, C. C., M. C. Kennicutt II, and J. M. Brooks(1985), Errors associated with the standard fluorimetric determination of chlorophylls and phaeopigments. Marine Chemistry, Vol. 17, pp. 1-12. https://doi.org/10.1016/0304-4203(85)90032-5
  55. Welschmeyer, N. A.(1994), Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and pheopigments. Limnology Oceanography, Vol. 39, pp. 1985-1992. https://doi.org/10.4319/lo.1994.39.8.1985
  56. Wright, S. W., S. W. Jeffrey, R. F. C. Mantoura, C. A. Llewellyn, T. B. Bjornland, D. Repeta, and N. Welschmeyer(1991), Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton. Marine Ecology Progress Series, Vol. 77, pp. 183-196. https://doi.org/10.3354/meps077183
  57. Yoo, K. I. and J. H. Lee(1979), Environmental studies of the Jinhae Bay. 1. Annual cycle of phytoplankton in relation to phytoplankton population dynamics. The Journal of the Oceanological Society of Korea, Vol. 14, pp. 26-31.
  58. Yoo, M. H., T. Y. Song, E. S. Kim, and J. K. Choi(2007), The caracteristics on the spatial and temporal distribution of phytoplankton in the Western Jinhae Bay, Korea. Journal of the Korean Society of Oceanography, Vol. 12, pp. 305-314.
  59. Yoon, Y. H.(2000), Variational characteristics of water quality and chlorophyll a concentration in the Northern Kamak Bay. Southern Korea. Journal of Environmental Science International, Vol. 9, pp. 429-436.
  60. Yoon, Y. H., H. K. Rho, and Y. K Kim(1992), Seasonal succession of phytoplankton population in the Hamdok Port, Northern Cheju Island. Cheju Mat. University, Vol. 16, pp. 27-42.
  61. Yoon, Y. H., J. S. Park, H. Y. Soh, and D. J. Hwang(2005), On the marine environment and distribution of phytoplankton community in the Northern East China sea in early summer. Journal of the Korean Society for Marine Environment & Energy, Vol. 8, pp. 100-110.
  62. Yoon, Y. H., J. S. Park, Y. G. Park, and I. H. Noh(2007), Marine environment and the distribution of phytoplankton community in the southwestern sea of Korea in summer 2005. Journal of the Korean Society for Marine Environmental Engineering, Vol. 10, pp. 155-166.
  63. Zapata, M., F. Rodriguez, and J. L. Garrido(2000), Separation of chlorophylls and carotenoids from marine phytoplankton: A new HPLC method using a reversed phase C8 column and pyridine containing mobile phases. Marine Ecology Progress Series, Vol. 195, pp. 29-45. https://doi.org/10.3354/meps195029
  64. Zhu, Z. Y., W. M. Ng, S. M. Liu, J. Zhang, J. C. Chen, and Y. Wu(2009), Estuarine phytoplankton dynamics and shift of limiting factors: A study in the Changjiang (Yangtze River) Estuary and adjacent area. Estuarine, Coastal and Shelf Science, Vol. 84, pp. 393-401. https://doi.org/10.1016/j.ecss.2009.07.005