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플라즈마 처리수와 과산화수소 희석수 침지에 따른 상추의 생육 및 바이오 활성 물질 함량 변화

Changes in the Growth and Bioactive Compounds Content of Lettuce Soaked in Plasma Activated Water and Hydrogen Peroxide Dillution Water

  • 안영휘 (충남대학교 바이오 AI 융합학과) ;
  • 노승원 (충남대학교 바이오 AI 융합학과) ;
  • 도종원 (한국농어촌공사 농어촌연구원 통합물관리지원단) ;
  • 박종석 (충남대학교 농업생명과학대학 원예학과)
  • Ahn, Young Hwi (Graduate school of Bio-AI Convergence, Chungnam National University) ;
  • Noh, Seung Won (Graduate school of Bio-AI Convergence, Chungnam National University) ;
  • Do, Jong Won (Integrated Water Management Supporting Department, Rural Research Institute, Korea Rural Community Corporation) ;
  • Park, Jong Seok (Department of Horticultural Science, Chungnam National University)
  • 투고 : 2021.04.06
  • 심사 : 2021.04.19
  • 발행 : 2021.04.30

초록

본 연구는 플라즈마 발생 장치를 수경재배 시스템과 결합하여 상추를 재배하고 수확 후 플라즈마 활성수와 과산화수소 0, 3, 6% 농도에 침지 처리를 하여 생육량 및 바이오 활성 물질 함량을 비교하였다. 고농도의 과산화수소로 인하여 근권부 생체중이 저해되는 결과를 나타냈으며, 엽장과 근권부 생체중을 제외하고는 유의적 차이가 발생하지 않았다. 상추 지상부의 chlorogenic acid와 4-hydroxy3-benzoic acid, 4-hydroxy benzoic acid 그리고 지상부 총 페놀 함량은 과산화수소 6%에서 유의적으로 가장 높았다. 하지만 근권부에서 측정된 이차대사산물인 quercetin 및 근권부 총 페놀 함량은 과산화수소 처리구보다 플라즈마 활성수에서 유의하게 높았다. 과산화수소 처리 기간 동안 상추의 뿌리는 직접적인 피해를 받으며 괴사하였으나, 잎에서는 과산화수소 6% 처리에서 바이오 활성 물질이 증가하는 결과를 나타내었다. 플라즈마 활성수 처리 상추는 생리 장해가 발생하지 않았으며, 과산화수소 6% 처리와 유사한 양의 이차대사산물 증대 효과를 나타내었다. 또한, 뿌리의 바이오 활성 물질 함량이 가장 높았던 결과 등을 고려할 때 근채류 및 엽채류의 수경재배에 플라즈마 기술을 적용할 시 작물의 바이오 활성 물질 함량을 증대시키는 데 효과적일 것으로 판단된다.

In this study, a plasma generating device was combined with a hydroponics system to grow lettuce. After harvest, we compared contents of bioactive compounds in lettuce by soaking in plasma-activated water and 0, 3, 6% of hydrogen peroxide. High concentrations of hydrogen peroxide resulted in inhibition of root fresh weight, and no significant difference occurred except for leaf length and root fresh weight. Shoot chlorogenic acid content and shoot 4-hydroxy3-benzoic acid content, shoot 4-hydroxy benzoic acid content and shoot total phenolic content were significantly higher in 6% hydrogen peroxide, and root quercetin content and root total phenolic content were significantly higher in plasma activated water. Root zone was necrosis due to hydrogen peroxide, while shoot phenol contents in 6% hydrogen peroxide were highly increased. Plasma-activated water had no physiological disturbances, has an effect of increasing bioactive compounds material as 6% hydrogen peroxide. It was considered effective in increasing the bioactive contents of the crops when plasma technique is applied to hydroponic cultivation of root and leaf vegetables.

키워드

과제정보

이 논문은 2021년도 정부(과학기술정보통신부)의 재원으로 정보통신기획평가원의 지원을 받아 수행된 연구임(No. 2020-0-01441, 인공지능융합연구센터지원(충남대학교)).

참고문헌

  1. Baek K.H., K.W. Oh, W.Y. Lee, S.S. Lee, M.K. Kim, H.S. Kwon, E.J. Rhee, J.H. Han, K.H. Song, B.Y. Cha, K.W. Lee, and M.I. Kang 2010, Association of oxidative stress with postmenopausal osteoporosis and the effects of hydrogen peroxide on osteoclast formation in human bone marrow cell cultures. Calcified Tissue International 87:226-235. doi:10.1007/s00223-010-9393-9
  2. Bekeschus S., A. Schmidt, K.D. Weltmann, and T. von Woedtke 2016, The plasma jet kINPen-A powerful tool for wound healing. Clinical Plasma Medicine 4:19-28. doi:10.1016/j.cpme.2016.01.001
  3. Blum J., B. Lemaire, and S. Lafay 2007, Effect of a green decaffeinated coffee extract on glycaemia. NutraFoods Res 6:13-7.
  4. Bray E.A. 1997, Plant responses to water deficit. Trends in Plant Science 2:48-54. doi:10.1016/S1360-1385(97)82562-9
  5. Chen X.Y., X. Ding, S. Xu, R. Wang, W. Xuan, Z.Y. Cao, J. Chen, H.H. Wu, M.B. Ye, and W.B Shen 2009, Endogenous hydrogen peroxide plays a positive role in the upregulation of heme oxygenase and acclimation to oxidative stress in wheat seedling leaves. Journal of Integrative Plant Biology 51:951-960. doi:10.1111/j.1744-7909.2009.00869.x
  6. Dat J., S. Vandenabeele, E. Vranova, M. Van Montagu, D. Inze, and F. Van Breusegem 2000, Dual action of the active oxygen species during plant stress responses. Cellular and Molecular Life Sciences CMLS 57:779-795. doi:10.1007/s000180050041
  7. Dempsey D.A., and D.F. Klessig 1995, Signals in plant disease resistance. Bulletin de l'Institut Pasteur 93:167-186. doi:10.1016/0020-2452(96)81488-6
  8. Deng X.P., Y.J. Cheng, X.B. Wu, S.S. Kwak, W. Chen, and A.E. Eneji 2012, Exogenous hydrogen peroxide positively influences root growth and exogenous hydrogen peroxide positively influences root growth and metabolism in leaves of sweet potato seedlings. Australian Journal of Crop Science 6:1572.
  9. Deng X., J. Shi, and M.G. Kong 2006, Physical mechanisms of inactivation of Bacillus subtilis spores using cold atmospheric plasmas. IEEE Transactions on Plasma Science 34:1310-1316. doi:10.1109/TPS.2006.877739
  10. Dixon R.A., and N.L. Paiva 1995, Stress-induced phenylpropanoid metabolism. The Plant Cell 7:1085. doi:10.1105/tpc.7.7.1085
  11. Dunand C., M. Crevecoeur, and C. Penel 2007, Distribution of superoxide and hydrogen peroxide in Arabidopsis root and their influence on root development: possible interaction with peroxidases. New Phytologist 174:332-341. doi:10.1111/j.1469-8137.2007.01995.x
  12. Dupont S., Z. Mondin, G. Willamson, and K. Price 2000, Effect of variety, processing, and storage on the flavonoid glycoside and composition of lettuce and chicory. Journal of Agricultural and Food Chemistry 48:3957-3964. doi:10.1021/jf0002387
  13. Eliasson B., W. Egli, and U. Kogelschatz 1994, Modelling of dielectric barrier discharge chemistry. Pure and Applied Chemistry 66:1275-1286. doi:10.1351/pac199466061275
  14. Farouk S., and A.M.S. Abdul Qados 2018, Enhancing seed quality and productivity as well as physio-anatomical responses of pea plants by folic acid and/or hydrogen peroxide application. Scientia Horticulturae 240:29-37. doi:10.1016/j.scienta.2018.05.049
  15. Foreman J, J.H. Bothwell, V. Demidchik, P. Mylona, H. Miedema, M.A. Torres, P. Linstead, S. Costa, C. Brownlee, J.D.G. Jones, J.M. Davies, and L. Dolan 2003, Reactive oxygen species produced by NADPH oxidase regulate plant cell growth. Nature 422:442-446. doi:10.1038/nature01485
  16. Frohling A., M. Baier, J. Ehlbeck, D. Knorr, and O. Schluter 2012, Atmospheric pressure plasma treatment of Listeria innocua and Escherichia coli at polysaccharide surfaces: inactivation kinetics and flow cytometric characterization. Innovative Food Science & Emerging Technologies 13:142-150. doi:10.1016/j.ifset.2011.11.002
  17. Hayashi N., T. Nakashima, and A. Yonesu 2011, Sterilization of medical equipment using air torch plasma produced by microwave discharge. IEEE Transactions on Plasma Science 39:2976-2977. doi:10.1109/TPS.2011.2145004
  18. Hoek-van den Hil E.F., E.M. van Schothorst, I. van der Stelt, H.J. Swarts, D. Venema, M. Sailer, J.J.M. Vervoort, P.C.H. Hollman, I.M.C.M. Rietjens, and J. Keijer 2014, Quercetin decreases high-fat diet induced body weight gain and accumulation of hepatic and circulating lipids in mice. Genes & Nutrition 9:418. doi:10.1007/s12263-014-0418-2
  19. Hoek-van den Hil E.F., J. Keijer, A. Bunschoten, J.J.M. Vervoort, B. Stankova, M. Bekkenkamp, L. Herreman, D. Venema, P.C.H. Hollman, E. Tvrzicka, I.M.C.M. Rietjens, and E.M. van Schothorst 2013, Quercetin induces hepatic lipid omega-oxidation and lowers serum lipid levels in mice. PloS One 8:e51588. doi:10.1371/annotation/53e96376-38fe-40e7-b73a-60e7232cef0e
  20. Inden F. 1982, Management of pH in nutrient solution. J. Jap. Agri. & Hort 57:327-331.
  21. Islam A.K.M.S., D.G. Edwards, and C.J. Asher 1980, pH optima for crop growth. Plant and Soil 54:339-357. doi:10.1007/BF02181830
  22. Kim D.S., and Y.S. Park 2012, Change of hydroponic components by plasma treatment. Journal of the Environmental Sciences 21:363-368. https://doi.org/10.5322/JES.2012.21.3.363
  23. Kim J.H., M.A. Lee, G.J. Han, and B.H. Cho 2014, Plasma in dentistry: a review of basic concepts and applications in dentistry. Acta Odontologica Scandinavica 72:1-12. doi: 10.3109/00016357.2013.795660
  24. Lau V., and N. Mattson 2021, Effects of hydrogen peroxide on organically fertilized hydroponic lettuce (Lactuca sativa L.). bioRxiv (in press). doi:10.1101/2021.01.01.425018
  25. Lee S.H., A.P. Singh, and G.C. Chung 2004, Rapid accumulation of hydrogen peroxide in cucumber roots due to exposure to low temperature appears to mediate decreases in water transport. Journal of Experimental Botany 55:1733-1741. doi:10.1093/jxb/erh189
  26. Lee W.H., B.I. Lee, K.D. Kim, J.W. Lee, and Y.S. Kwon 1998, Nitrate content and activities of nitrate reductase and glutamine synthetase as affected by temperature and pH of nutrient solution in leaf lettuce and water dropwort. Korean Society For Horticultural Science 39:157-160.
  27. Mittler R. 2002, Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 7:405-410. doi:10.1016/S1360-1385(02)02312-9
  28. Mok Y.S., D.J. Koh, D.N. Shin, and K.T. Kim 2004, Reduction of nitrogen oxides from simulated exhaust gas by using plasma-catalytic process. Fuel Processing Technology 86:303-317. doi:10.1016/S0140-6701(05)80860-4
  29. Moreau M., N. Orange, and M.G.J. Feuilloley 2008, Non-thermal plasma technologies: new tools for bio-decontamination. Biotechnology Advances 26:610-617. doi:10.1016/j.biotechadv.2008.08.001
  30. Moskova I., D. Todorova, V. Alexieva, S. Ivanov, and I. Sergiev 2009, Effect of exogenous hydrogen peroxide on enzymatic and nonenzymatic antioxidants in leaves of young pea plants treated with paraquat. Plant Growth Regul 57:193-202. doi:10.1007/s10725-008-9336-x
  31. Nadkarni A.N. 1989, Indian materia medica. Popular Book Depot, Bombay India. 1:619.
  32. Ngo Thi M.H., P.L. Shao, J.D. Liao, C.C.K Lin, and H.K. Yip 2014, Enhancement of angiogenesis and epithelialization processes in mice with burn wounds through ROS/RNS signals generated by non-thermal N2/Ar micro-plasma. Plasma Processes and Polymers 11:1076-1088. doi:10.1002/ppap.201400072
  33. Nicolle C., N. Cardinault, E. Gueux, L. Jaffrelo, E. Rock, A. Mazur, P. Amouroux, and C. Remesy 2004, Health effect of vegetable-based diet: Lettuce consumption improves cholesterol metabolism and antioxidant status in the rat. Clinical Nutrition 23:605-614. doi:10.1016/j.clnu.2003.10.009
  34. Noctor G., and C.H. Foyer 1998, Ascorbate and glutathione: keeping active oxygen under control. Annu. Rev. Plant Physiol. Plant Mol. Biol 49:249-279. doi:10.1146/annurev.arplant.49.1.249
  35. Noh S.W., J.S. Park, S.J. Kim, D.W. Kim, and W.S. Kang 2020, Effect of Plasma-activated Water Process on the Growth and Functional Substance Content of Lettuce during the Cultivation Period in a Deep Flow Technique System. Protected Horticulture and Plant Factory. 29:464-472. (in Korean) doi:10.12791/KSBEC.2020.29.4.464
  36. Peng C.L., Z.Y. Ou, N. Liu, and G.Z. Lin 2005, Response to high temperature in flag leaves of super high-yielding rice Pei'ai 64S/E32 and Liangyoupeijiu. Rice Sci 12:179-186.
  37. Schutzendubel A., P. Nikolova, C. Rudolf, and A. Polle 2002, Cadmium and H2O2-induced oxidative stress in Populus × canescens roots. Plant Physiology and Biochemistry 40: 577-584. doi:10.1016/s0981-9428(02)01411-0
  38. Serafini M., R. Bugianesi, M. Salucci, E. Azzini, A Raguzzini, and G. Maiani 2002, Effect of acute ingestion of fresh and stored lettuce (Lactuca sativa) on plasma total antioxidant capacity and antioxidant levels in human subjects. British Journal of Nutrition 88:615-623. doi:10.1079/BJN2002722
  39. Sharma P., A.B. Jha, R.S. Dubey, and M. Pessarakli 2012, Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany 2012:1-26. doi:10.1155/2012/217037
  40. Shimoda H., E. Seki, and M. Aitani 2006, Inhibitory effect of green coffee bean extract on fat accumulation and body weight gain in mice. BMC Complementary and Alternative Medicine 6:1-9. doi:10.1186/1472-6882-6-9
  41. Trejo-Tellez L.I., and F.C. Gomez-Merino 2012, Nutrient solutions for hydroponic systems. In Hydroponics: A standard methodology for plant biological researches. Intech. Rijecka, Croatia 1-22. doi:10.5772/37578
  42. You J.H., J.H. Lee, S.H. Mun, S.R. Kwon, T.S. Park, and J.Y. Kwon 2020, Disinfection effect of corona discharged plasma water on fish pathogens. Journal of Fish Pathology 33:63-69. (in Korean) doi:10.7847/jfp.2020.33.1.063
  43. Zhang M., R. DAI, S. Zhang, and H. Jiang 2017, Alleviation effects of seed soaking with H2O2 on seed germination in rape under low temperature stress. Journal of Nanjing Agricultural University 40:963-970. doi:10.7685/jnau.201702029