DOI QR코드

DOI QR Code

Antimicrobial Resistance and Genetic Characterization of Pathogenic Escherichia coli Isolated from Distribution Beef in Gwangju

광주지역 유통·판매 소고기에서 분리된 병원성대장균의 항생제 내성 및 유전적 특성조사

  • Received : 2021.03.19
  • Accepted : 2021.04.18
  • Published : 2021.04.30

Abstract

This study aims at investigating occurrence of pathogenic Escherichia coli (E. coli) associated with high rate of food poisoning in beef samples collected from markets in Gwangju, and the antimicrobial resistance and genetic characterization of the isolated strains. Pathogenic E. coli was detected in 82 (24.4%) of 335 beef samples, and 102 strains were isolated. Of 102 strains, 66 were identified as enterohemorrhagic E. coli (EHEC) and 11 strains harbored both pathogenic genes of EHEC and EPEC. In antimicrobial susceptibility test, 30 strains were resistant to more than 1 antimicrobial. The most frequent antimicrobial resistance observed in the isolated E. coli was to tetracycline, followed by ampicillin, trimethoprim/sulfamethoxazole, and chloramphenicol. Serotyping of isolated E. coli identified serogroups as O26, O91, O103, O104, O111, O113, O121, O128 and O145. Therefore, we suggest that hygienic management of cooking utensils and sufficient cooking are needed so as not to cause cross-contamination when cooking beef.

본 연구는 광주광역시에 유통·판매되고 있는 소고기를 대상으로 식중독 발생 비율이 높은 병원성대장균의 검출 여부와 분리된 균주의 항생제 내성 및 유전적 특성을 조사하였다. 전체 335건의 소고기 중 82건에서 병원성대장균이 검출(24.4%)되었고 102개의 다양한 균주를 분리하였다. 분리균주의 병원성 유전자를 토대로 분류한 결과, EHEC가 66균주로 가장 많았고 EHEC와 EPEC 등 2가지 유전자가 동시에 검출된 균주도 11균주가 있었다. 분리된 균주의 항생제 내성 시험결과, 30균주는 1가지 이상의 항생제에 대하여 내성을 보였다. 그 중 tetracycline에 내성을 보이는 균주가 27균주로 가장 많았고 그 외에 ampicillin, trimethoprim/sulfamethoxazole, chloramphenicol 등의 순으로 많았다. 분리된 균주의 혈청형 검사 결과, 혈청형은 O26, O91, O103, O104, O111, O113, O121, O128 및 O145로 확인되었다. 따라서 소고기의 조리 가공 시 교차오염이 발생하지 않도록 조리기구 등에 대한 위생적 관리와 충분한 조리 등의 식중독 예방을 위한 주의가 필요할 것으로 생각된다.

Keywords

References

  1. Madigan, M.T., Martinko J.M., Parker, J., 2000. Brock biology of microorganisms. 9th ed, Prentice-hall, NJ, USA, pp. 986-987.
  2. Kim, M.S., Kim, O.M., Kim, I.K., Lee, K.I., Lee, S.H., Lee, J.G., Lim, S.M., 2002. Food hygienics, Hoonminsa, Seoul, Korea, pp. 62-107.
  3. Ewing, W.H., 1986. Edwards and Ewing's identification of enterobacteriaceae, 4th ed. Elsevier Science Publish, NY, USA, pp. 181-318.
  4. Paton, J.C., Paton, A.W., Pathogenesis and diagnosis of shiga toxin producing Escherichia coli infections. Clin. Microbiol. Rev., 11, 450-479 (1998). https://doi.org/10.1128/cmr.11.3.450
  5. Bekal, S., Brousseau, R., Masson, L., Prefontaine, G., Fair-brother, J., Harel, J., Rapid identification of Escherichia coli pathotypes by virulence gene detection with DNA microarrays. J. Clin. Microbiol., 41, 2113-2115 (2003). https://doi.org/10.1128/JCM.41.5.2113-2125.2003
  6. Zweifel, C., Blanco, J.E., Blance, M., Blanco, J., Stephan, R., Serotypes and virulence genes of ovine non-O157 Shiga toxin-producing Escherichia coli in switzerland. Int. J. Food Microbiol., 95, 19-27 (2004). https://doi.org/10.1016/j.ijfoodmicro.2004.01.015
  7. U.S Food & Drug Administration, (2021, March 15). BAM Chapter 4A: Diarrheagenic Escherichia coli. Retrieved from https://www.fda.gov/food/laboratory-methods-food/bam-chapter-4a-diarrheagenic-escherichia-coli
  8. Yoo, Y.A., Kim, M.S., Kim, K.S., Park, S.H., Jumg, S.K., Antimicrobial resistance and implicated genes of E. coli isolated from commercial and cooked food in Seoul. J. Food Hyg. Saf., 25, 220-225 (2010).
  9. Kim, S.H., Park, Y.H., (2021, April 19). Antimicrobial resistance and food safety. Retrieved from https://www.korea-science.or.kr/article/JAKO200818259610202.pdf
  10. Kim, D.M., Improvement of enrichment for the detection of pathogenic Escherichia coli. Master's thesis, Korea University, Seoul, Korea (2016).
  11. National institute of infectious diseases (NIID), (2021, January 11). Enterohemorrhagic Escherichia coli (EHEC) infection, as of April 2017, Japan. Retrieved from https://www.niid.go.jp/niid/en/865-iasr/7282-447te.html
  12. Duffy, G., Cummins, E., Nally, P., O' Brien, S., Butler, F., A review of quantitative microbial risk assessment in the management of E.coli O157:H7 on beef. Meat Sci., 74, 76-88 (2006). https://doi.org/10.1016/j.meatsci.2006.04.011
  13. Ministry of Food and Drug Safety, (2021, January, 11), Food poisoning statistics. Retrieved from https://www.foodsafetykorea.go.kr/portal/healthyfoodlife/foodPoisoningStat.do?menu_no=3724&menu_grp=MENU_NEW02&menu_no=3724&menu_grp=MENU_NEW02
  14. Ministry of Food and Drug Safety, (2021, January 11), Beware of food poisoning with pathogenic E. coli in summer. Retrieved from https://www.foodsafetykorea.go.kr/portal/board/boardDetail.do?menu_no=2859&bbs_no=bbs082&ntctxt_no=1074833&menu_grp=MENU_NEW05
  15. Korea Rural Economic Institute, 2019. 2018 Food balance sheet, Naju, Korea, pp. 24-151.
  16. Ministry of Agriculture, Food and Rural Affairs, 2020. National antibiotic use and resistance monitoring, Sejong, Korea, pp. 97-106.
  17. Ministry of Food and Drug Safety, 2020. Detection method for foodborne pathogens investigation (2021). Osong, Korea, pp. 11-57.
  18. Ministry of Food and Drug Safety, 2020. Korea food code (2020). Osong, Korea, pp. 263-328.
  19. Valadez, A.M., Debroy, C., Dudley, E., Cutter, C.N., Multiplex PCR detection of Shiga toxin-producing Escherichia coli strains belonging to serogroups O157, O103, O91, O113, O145, O111 and O26 experimentally inoculated in beef carcass swabs, beef trim, and ground beef. J. Food Prot., 74, 228-239 (2011). https://doi.org/10.4315/0362-028X.JFP-10-386
  20. Paddock, Z., Shi, X., Bai, J., Nagaraja, T.G., Applicability of a multiplex PCR to detect O26, O45, O103, O111, O121, O145 and O157 serogroups of Escherichia coli in cattle feces. Vet. Microbiol., 156, 381-388 (2012). https://doi.org/10.1016/j.vetmic.2011.11.017
  21. DebRoy, C., Roverts, E., Kundrat, J., Davis, M.A., Briggs, C.E., Fratamico, P.M., Detection of Escherichia coli serogroups O26 and O113 by PCR amplification of the wzx and wzy genes. Appl. Environ. Microbiol., 70, 1830-1832 (2004). https://doi.org/10.1128/AEM.70.3.1830-1832.2004
  22. Lee, D.Y., Lee, J.Y., Wang, H.J., Shin, D.B., Cho, Y.S., Prevalence and classification of Escherichia coli isolated from bibimbap in Korea. Korean J. Food Sci. Technol., 47, 126-131 (2015). https://doi.org/10.9721/KJFST.2015.47.1.126
  23. Clinical and Laboratory Standards Institute, (2021, March 15). CLSI M100-ED29:2019 Performance Standards for Antimicrobial Susceptibility Testing, 29th Edition. Retrieved from http://em100.edaptivedocs.net/GetDoc.aspx?doc=CLSIM100ED29:2019&sbssok=CLSI M100 ED29:2019 TABLE2A
  24. Cho, Y.S., Koo, M.S., Jang, H.J., Characterization of Diarrheagenic Escherichia coli Isolated from Fresh Beef, Pork, and Chicken Meat in Korean Markets. Microbiol. Biotechnol. Lett., 48, 121-128 (2020). https://doi.org/10.4014/mbl.1912.12005
  25. Kim, H.Y., Kim, E.J., Park, Y.C., Cho, J.I., Lee, J.O., Prevalence and characterization of enterohemorrhagic Escherichia coli(EHEC) isolated from ground beefs distributed in Gyeong-In region. Korean J. Food Sci. Technol., 38, 773-778 (2006).
  26. Pandal, N., Livrelli, V., Champs, C., Palcoux, J.B., Reyanaud, A., Scheutz, F., Sirot, J., Joly, B., Forestier, C., Prevalence and characterization of shiga toxin producing Escherichia coli isolated from cattle, food and children during a on year prospective study in france. J. Clin. Microbiol., 38, 1023-1031 (2000). https://doi.org/10.1128/JCM.38.3.1023-1031.2000
  27. Oh, Y.S., Lee, S.H., Hygienic quality of beef and distribution of pathogens during cut-meat processing. J. Food Hyg. Saf., 16, 96-102 (2001).
  28. Cho, S.H., Beak, S.H., Ahn, J.H., Nam, I.S., A study on microbial management level of manufacturing environment, raw meat and products in HACCP implemented meat market. Korean J. Org. Agric., 27, 193-204 (2019). https://doi.org/10.11625/KJOA.2019.27.2.193
  29. Kim, S.Y., Quality control study of ground meat. Master's thesis, Inje University, Busan, Korea (2003).
  30. Karmali, M.A., Gannon, V., Sargeant, J.M., Verocytotoxin-producing Escherichia coli (VTEC). Vet. Microbiol., 140, 360-370 (2010). https://doi.org/10.1016/j.vetmic.2009.04.011
  31. Jenkens, C., Perry, N.T., Cheasty, T., Shaw, D.J., Frankel, G., Dougan, G., Gunn, G.J., Smith, H.R., Paton, A.W., Paton, J.C., Distribution of the saa gene in strains of shiga toxin-producing Escherichia coli of human and bovine origins. J. Clin. Microbiol., 41, 1775-1778 (2003). https://doi.org/10.1128/JCM.41.4.1775-1778.2003
  32. Paton, A.W., Paton, J.C., Direct detection and characterization of Shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA, and saa. J. Clin. Microbiol., 40, 271-274 (2002). https://doi.org/10.1128/JCM.40.1.271-274.2002
  33. Kim, H.T., Lee, W.W., Jung, K.T., Lee, S.M., Son, E.J., Lee, G.R., Kim, G.H., Lee, D.S., Lee, K.W., Study on antimicrobial resistance of Escherichia coli isolated from domestic beef on sale. Korean J. Vet. Serv., 31, 17-29 (2008).
  34. Kim, H.T., Jung, K.T., Lee, D.S., Lee, K.W., Study on antimicrobial resistance of Escherichia coli isolated from domestic beef on sale (2). Korean J. Vet. Serv., 32, 93-102 (2009).
  35. Chea, H.S., Kim, N.H., Han, H.J., Son, H.R., Kim, C.K., Kim, S.H., Lee, J.H., Kim, J.T., Characterization and isolation of shiga toxin-producing Escherichia coli from Bovine feces and carcass. Korean J. Vet. Serv., 32, 241-249 (2009).
  36. Asai, T., Kojima, A., Harada, K., Ishihara, K., Takahashi, T., Tamura, Y., Correlation between the usage volume of veterinary therapeutic antimicrobials and resistance in Escherichia coli isolated from the feces of food-producing animals in Japan. Jpn. J. Infect. Dis., 58, 369-372 (2005).
  37. Lim, S.K., Nam, H.M., Moon, D.C., Jang, G.C., Jung, S.C., Korean veterinary antimicrobial resistance monitoring group., Antimicrobial resistance of Escherichia coli isolated from healthy animals during 2010-2012. Korean J. Vet. Res., 54, 131-137 (2014). https://doi.org/10.14405/kjvr.2014.54.3.131
  38. World Health Organization, 2011. Report of the 1st meeting of the WHO Advisory group on integrated surveillance of antimicrobial resistance, 2009. World Health Organization, Geneva, Switzerland, pp. 18-33.
  39. Centers for Disease Control and Prevention, 2018 National Shiga toxin-producing Escherichia coli (STEC) surveillance annual report, 2016. US Department of Health and Human Services, Atlanta, Georgia, USA.
  40. European Food Safety Authority and European Centre for Disease Prevention and Control, The European union one health 2018 zoonoses report, EFSA J., 17, 5926 (2019).
  41. Yun, Y.S., Kim, N.O., Hong, S.H., Chun, J.H., Hwang, K.J., The prevalence of pathogenic Escherichia coli isolated by the enteric pathogens active surveillance network (Enter-Net), 2010-2019. Public Heath Weekly Report, 13, 2867-2870 (2020).
  42. Kwak, H.S., Cha, J., Kwang, K.J., Kim, H., Park, S.H., Kim, C.M., Characterization of verotoxin-producing Escherichia coli isolated from domestic foods. J. Food Hyg. Saf., 15, 241-247 (2000).
  43. Park, S.H., Kim, S.H., Seo, J.J., Kee, H.Y., Kim, M.J., Seo, K.W., Lee, D.H., Choi, Y.H., Lim, D.J., Hur, Y.J., Cho, S.H., Lee, B.K., An outbreak of inapparent non-O157 enterohemorrhagic Escherichia coli infection. Korean J. Med., 70, 495-504 (2006).