Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Adjuvant therapy with 1% alendronate gel for experimental periodontitis treatment in rats

  • de Campos Kajimoto, Natalia (Department of Periodontics, The University of Texas Health Science Center at San Antonio) ;
  • de Paiva Buischi, Yvonne (Department of Periodontics, The University of Texas Health Science Center at San Antonio) ;
  • Loomer, Peter Michael (Department of Periodontics, The University of Texas Health Science Center at San Antonio) ;
  • Bromage, Timothy G. (Department of Biomaterials & Biomimetics, New York University College of Dentistry) ;
  • Ervolino, Edilson (Division of Histology, Department of Basic Sciences, Sao Paulo State University (UNESP), School of Dentistry) ;
  • Fucini, Stephen Enrico ;
  • Pola, Natalia Marcumini (Division of Periodontics, Department of Semiology and Clinic, Federal University of Pelotas (UFPEL), School of Dentistry) ;
  • Pirovani, Beatriz Ommati (Division of Periodontology, Department of Surgery and Integrated Clinic, Sao Paulo State University (UNESP), School of Dentistry) ;
  • Morabito, Maria Juliana Sismeiro (Division of Periodontology, Department of Surgery and Integrated Clinic, Sao Paulo State University (UNESP), School of Dentistry) ;
  • de Almeida, Juliano Milanezi (Division of Periodontology, Department of Surgery and Integrated Clinic, Sao Paulo State University (UNESP), School of Dentistry) ;
  • Furlaneto, Flavia Aparecida Chaves (Division of Periodontology, Departament of Oral Maxillofacial Surgery and Periodontology, University of Sao Paulo (USP), School of Dentistry) ;
  • Nagata, Maria Jose Hitomi (Division of Periodontology, Department of Surgery and Integrated Clinic, Sao Paulo State University (UNESP), School of Dentistry)
  • Received : 2021.02.03
  • Accepted : 2021.05.10
  • Published : 2021.12.31
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Abstract

Purpose: The aim of this study was to evaluate the effects of locally delivered 1% alendronate (ALN) gel used as an adjunct to non-invasive periodontal therapy. Methods: Ligature-induced periodontitis was performed in 96 rats. The ligature was tied in the cervical area of the mandibular left first molar. The animals were randomly divided into 4 groups: 1) NT, no treatment; 2) SRP, scaling and root planning; 3) SRP/PLA, SRP followed by filling the periodontal pocket with placebo gel (PLA); and 4) SRP/ALN, SRP followed by filling the periodontal pockets with 1% ALN gel. Histomorphometric (percentage of bone in the furcation region [PBF]) and immunohistochemical (receptor activator of nuclear factor-κB ligand, osteoprotegerin, and tartrate-resistant acid phosphatase) analyses were performed. Data were statistically analyzed, with the threshold of statistical significance set at P≤0.05. Results: The SRP, SRP/PLA, and SRP/ALN groups presented a higher PBF than the NT group (P≤0.01) at 7, 15, and 30 days. The SRP/ALN group presented a higher PBF than the SRP/PLA group in all experimental periods, as well as a higher PBF than the SRP group at 15 and 30 days. No differences were observed in the immunohistochemical analyses (P>0.05 for all). Conclusions: Locally delivered 1% ALN gel used as an adjunct to SRP enhanced bone regeneration in the furcation region in a rat model of experimental periodontitis.

Keywords

Acknowledgement

Maria Juliana S. D. Morabito received a scholarship from the Sao Paulo Research Foundation (FAPESP, Sao Paulo, SP, Brazil, Grant #18/03331-1). Dr. Natalia de Campos received a scholarship from the Coordination for the Improvement of Higher Education Personnel (CAPES, Brasilia, DF, Brazil).

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