DOI QR코드

DOI QR Code

광주지역 먹는물 공동시설의 미생물 특성 및 분포조사

Evaluation of Characteristics of Microorganisms Isolated from Public Drinking Water Facilities in Gwangju City

  • 박주현 (광주광역시보건환경연구원) ;
  • 김선정 (광주광역시보건환경연구원) ;
  • 이윤국 (광주광역시보건환경연구원) ;
  • 김난희 (광주광역시보건환경연구원) ;
  • 강유미 (광주광역시보건환경연구원) ;
  • 배석진 (광주광역시보건환경연구원) ;
  • 김종민 (광주광역시보건환경연구원)
  • Park, Juhyun (Health and Environment Research Institute of Gwangju) ;
  • Kim, Seonjung (Health and Environment Research Institute of Gwangju) ;
  • Lee, Youn-gook (Health and Environment Research Institute of Gwangju) ;
  • Kim, Nanhee (Health and Environment Research Institute of Gwangju) ;
  • Kang, Yumi (Health and Environment Research Institute of Gwangju) ;
  • Bae, Seokjin (Health and Environment Research Institute of Gwangju) ;
  • Kim, Jongmin (Health and Environment Research Institute of Gwangju)
  • 투고 : 2021.03.05
  • 심사 : 2021.03.29
  • 발행 : 2021.04.30

초록

Objective: This study was performed to detect indicator bacteria in drinking spring water samples in Gwangju City and to identify their genus using the VITEK-II system. Methods: The subjects were ten drinking spring water sites in Gwangju. Samples of spring water were taken every month from September 2019 to August 2020. We analyzed for the indicator bacteria Yersinia and microorganisms isolated from the spring water. Result: According to the research results on indicator bacteria, general bacteria in st1-st7 with sterilization facilities in the spring and summer were investigated in the range of 0-2 CFU/mL and 0-12 CFU/mL. In st9, where a sterilization facility was not installed, the most general bacteria were detected (160 CFU/mL). Total coliform and fecal coliform showed unsatisfied rates of 16.7 and 11.1% in spring and 14.7 and 11.8% in summer, respectively. The unsatisfied rates of total coliform for the designated and non-designated spring water facilities were 3.8 and 47.1%, respectively, and for the fecal coliform group they were 2.5 and 35.3%. The difference was confirmed according to the presence of a sterilization facility. Yersinia spp. was not detected in all drinking spring water. Forty-one strains in 25 species were isolated from ten sites. The results classified as major dominant species are Pseudomonas spp. 14.6%, Pantoea spp. 9.8%, Serratia spp. 9.8%, Acinetobacter spp. 9.8%, Citrobacter spp. 7.3%, Bordetella spp. 7.3%, Delftia spp. 4.9%, and Enterobacter spp. 4.9%. Conclusions: Based on the result that various species derived from fecal pollution and artificial pollutants were detected in the non-specified public spring water facilities that many people use, the facilities need institutional complements such as continuous management or complete shutdowns.

키워드

참고문헌

  1. Kim KA, Lee BO, Kim, OM, Hur MJ, Kim KT, Ro JI, et al. A study on pollution of spring in Incheon area. Journal of Environmental and Sanitary Engineering. 2007; 22(3): 35-50.
  2. Korea Ministry of Environment, White paper of Environment; 2020. p. 238.
  3. Krieg NR and Holt JG. Bergey's manual of systematic bacteriology. Williams and Wilkins, Baltimore; 1984. 498-506.
  4. Choi PK, Huh P, Lee KH, Cho DH, Kim CG, Kim TH. Study on water quality improvement in public drinking water facilities in Gyeonggi-do. Journal of Korean Society for Environmental Analysis. 2018; 21(3), 148-153.
  5. Cover Timothy L, ABER Robert C. Yersinia enterocolitica. New England Journal of Medicine, 1989; 321(1): 16-24. https://doi.org/10.1056/NEJM198907063210104
  6. Gonul Sahika Aktug, Karapinar Mehmet. The microbiological quality of drinking water supplies of Izmir City: the incidence of Yersinia enterocolitica. International Journal of Food Microbiology, 1991; 13(1): 69-73. https://doi.org/10.1016/0168-1605(91)90138-F
  7. Highsmith AK, Feeley JC, Skaliy P, Wells JG, Wood BT, Isolation of Yersinia enterocolitica from well water and growth in distilled water. Appl. Environ. Microbiol, 1977; 34: 745-748. https://doi.org/10.1128/AEM.34.6.745-750.1977
  8. Korea Ministry of Environment, Instruction of the Ministry of Environment 1374, Article4
  9. Korea Ministry of Environment, Standard Method for water Quality Pollution (ES 04130.1d); 2018.
  10. Korea Ministry of Environment, Standard Method for Drinking water quality (ES 05702.1b-05711.1c); 2018.
  11. Yoon TH, Lee HL, Choi SJ, Lee MY, EO SM. Occurrence of Indicator bacteria and Identification of total coliforms using 16s rRNA gene in Drinking spring water in Seoul. Journal of Environ Health Sci. 2013; 39(6): 513-521. https://doi.org/10.5668/JEHS.2013.39.6.513
  12. Lee YG, et al. Quality of spring water influenced by rainfall in mudeung mountain. J. of the Korea Society for Environmental Analysis. 2011; 14(3): 146-157.
  13. Lee YG, et al. Water quality of non-designated spring in Gwangju. J. of the Korea Society for Environmental Analysis. 2013; 16(2): 152-162.
  14. Scales, Brittan S, et al. Microbiology, genomics, and clinical significance of the Pseudomonas fluorescens species complex, an unappreciated colonizer of humans. Clinical Microbiology Reviews. 2014; 27(4): 927-948. https://doi.org/10.1128/CMR.00044-14
  15. Cruz, Andrea T, Andreea C. Cazacu, and Coburn H. Allen. Pantoea agglomerans, a plant pathogen causing human disease. Journal of Clinical Microbiology. 2007; 45(6): 1989-1992. https://doi.org/10.1128/JCM.00632-07
  16. Aljorayid, Abdullah, et al. Serratia fonticola, pathogen or bystander? A case series and review of the literature. IDCases 2016; 5: 6-8. https://doi.org/10.1016/j.idcr.2016.05.003
  17. Mahlen, Steven D. Serratia infections: from military experiments to current practice. Clinical Microbiology Reviews. 2011; 24(4): 755-791. https://doi.org/10.1128/CMR.00017-11
  18. Linde, Hans-Jorg, et al. Septicemia due to Acinetobacter junii. Journal of Clinical Microbiology. 2002; 40(7): 2696-2697. https://doi.org/10.1128/JCM.40.7.2696-2697.2002
  19. Ku SC, et al. Clinical and microbiological characteristics of bacteremia caused by Acinetobacter lwoffii. European Journal of Clinical Microbiology and Infectious Diseases. 2000; 19(7): 501-505. https://doi.org/10.1007/s100960000315
  20. Howard, Aoife, et al. Acinetobacter baumannii: an emerging opportunistic pathogen. Virulence. 2012; 3(3): 243-250. https://doi.org/10.4161/viru.19700