대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
권호 표지
DOI QR코드

DOI QR Code

금은화가 HUVEC에서 죽상동맥경화증 관련 염증인자 발현 억제에 미치는 영향

The Effect of Lonicera Japonica Thunberg on Inflammatory Factor Expression Associated with Atherosclerosis

  • 양지혜 (대전대학교 한의과대학 심계내과학교실) ;
  • 유호룡 (대전대학교 한의과대학 심계내과학교실) ;
  • 김윤식 (대전대학교 한의과대학 심계내과학교실) ;
  • 설인찬 (대전대학교 한의과대학 심계내과학교실)
  • Yang, Ji-hae (Dept. of Cardiology and Neurology of Korean Medicine, College of Korean Medicine, Dae-jeon University) ;
  • Yoo, Ho-ryong (Dept. of Cardiology and Neurology of Korean Medicine, College of Korean Medicine, Dae-jeon University) ;
  • Kim, Yoon-sik (Dept. of Cardiology and Neurology of Korean Medicine, College of Korean Medicine, Dae-jeon University) ;
  • Seol, In-chan (Dept. of Cardiology and Neurology of Korean Medicine, College of Korean Medicine, Dae-jeon University)
  • 투고 : 2021.02.18
  • 심사 : 2021.03.27
  • 발행 : 2021.03.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: The purpose of this study was to investigate the effect of Lonicera Japonica Thunberg (LJT) on the inflammatory factor expression associated with atherosclerosis in human umbilical vein endothelial cells (HUVECs). Methods: After treatment with LJT in HUVEC which is treated with TNF-α, we measured the expression levels of biomarkers (MCP-1, ICAM-1, VCAM-1, KLF2, and eNOS), mRNA (CCL2, ICAM1, VCAM1, KLF2, and NOS3), and the proteins (MCP-1, ICAM-1, VCAM-1, KLF2, eNOS, ERK, JNK, and p38). Results: 1. Compared to the control, LJT significantly reduced MCP-1 and VCAM-1 levels at concentrations of 100, 200, and 400 ㎍/ml and ICAM-1 expression at 200 and 400 ㎍/ml compared to the control. It increased KLF2 levels at all three concentrations, but not significantly, while eNOS expression was significantly increased at 400 ㎍/ml. 2. LJT was seen to significantly reduce the expression of CCL2, ICAM1, and VCAM1 mRNA at concentrations of 100, 200, and 400 ㎍/ml compared to the control. In contrast, significantly increased KLF2 and NOS3 mRNA levels were observed at 400 ㎍/ml and at 200 and 400 ㎍/ml, respectively. 3. Compared to the control, LJT significantly reduced the protein expression of MCP-1 and VCAM-1 at 200 and 400 ㎍/ml and of ICAM-1 at 400 ㎍/ml. In addition, it increased both KLF2 and eNOS protein levels at 200 and 400 ㎍/ml. Although LJT did not have an effect on ERK expression in comparison with the control, it significantly reduced JNK levels at 200 and 400 ㎍/ml and p38 levels at 400 ㎍/ml. Conclusions: These results suggest that LJT has an effect on the inhibition of inflammatory factor expression associated with atherosclerosis in HUVECs which could contribute to the prevention of cardiovascular and cerebrovascular diseases.

키워드

참고문헌

  1. Statistics Korea. Annual report on the causes of death statistics in 2019. Daejeon: Statistics Korea; 2020.
  2. World Health Organization. Global Health Observatory (GHO) data [Internet] Geneva: World Health Organization; 2016.
  3. Fauci A, Braunwald E, Kasper D, Hauser S, Longo D, Jameson J, et al. Harrison's principles of internal medicine. 17th edition. Seoul: MIP; 2010, p. 1815-9.
  4. Jeongdam Editorial Department. SIM Integrated internal medicine understood through anatomical pathophysiology 3: Cardiology. Seoul: Jeongdam; 2013, p. 205-8.
  5. Herbalism Compilation Committee. Herbalism. Seoul: Younglim Inc; 2013, p. 240-1.
  6. Bae JH, Kim MS, Kang EH. Antimicrobial Effect of Lonicerae Flos Extracts on Food-borne Pathogens. Korean J Food Sci Technol 2005;37(4):642-7.
  7. Suhr SS, Jung SK. Antiviral Effects of Fermented Lonicerae Flos on A Type Influenza Virus. Korean J Orient Int Med 2009;30(3):465-80.
  8. Kim JW, Choi HK, Shon YH, Lim JK, Lee HW, Nam KS. Chemopreventive Potential of Lonicerae flos Aqua-Acupuncture Solution. Kor J Pharmacogn 1999;30(3):261-8.
  9. Park HS. The Effects of Anti-cancer Response of Lonicerae Flos Herbalacupuncture. J Kor Acu Mox Soc 2005;22(5):91-7.
  10. Yun YG, Kim GM, Lee SJ, Ryu SH, Jang SI. Inhibitory Effect of Aqueous Extract from Lonicera japonica Flower on LPS-induced Inflammatory Mediators in RAW 264.7 Macrophages. The Kor J Herbology 2007;22(3):117-25.
  11. Yun KJ, Lee EY. Effects of Hot Aqueous and Ethanol Extract from Lonicera japonica Flos on NO and PGE2 in Macrophage. J Kor Acu Mox Soc 2012;29(1):67-74.
  12. Joo JS, Kim JS, Jeong JG, Kim BK. Study of Efficacy of Foeniculi Fructus and Lonicerae Flos Extract on Acute Pancreatitis. The Kor J Herbology 2010;25(4):39-45. https://doi.org/10.6116/KJH.2010.25.4.039
  13. Lee YW, Ahn SH, Kim HH, Kim KB. The Anti-Inflammatory Effect of Lonicera JaponicaGlycyrrhiza Uralensis Decoction on Ulcerative Colitis Induced by DSS in Mice. J Pediatr Korean Med 2018;32(3):16-25. https://doi.org/10.7778/JPKM.2018.32.3.0016
  14. Ju ST, Lee MJ, Lee HS, Jung HJ, Kim H, Kim JE. Screening of Anti-atherosclerotic Effect of Lonicera Flower by Antioxidative and Anti-thrombotic Mechanism. Kor J Oriental Med Phys & Path 2008;22(6):1509-17.
  15. Choi YS, Lee YW. Textbook of Cardiovascular Medicine. Seoul: Iljogak; 2010, p. 421-31.
  16. Kim CJ. Atherosclerosis and Inflammation. Kor J Lipidology 2001;11(4):413-9.
  17. Bergheanu SC, Bodde MC, Jukema JW. Pathophysiology and treatment of atherosclerosis. Neth Heart J 2017;25(4):231-42. https://doi.org/10.1007/s12471-017-0959-2
  18. Kim SH. Drug treatment of dyslipidemia. J Korean Med Assoc 2016;59(5):366-73. https://doi.org/10.5124/jkma.2016.59.5.366
  19. Committee for Guidelines for Management of Dyslipidemia. 2015 Korean Guideline for Management of Dyslipidemia. J Lipid Atheroscler 2015;4(1):61-92. https://doi.org/10.12997/jla.2015.4.1.61
  20. Choi HJ, Park JN. Management of Risk of Statin Therapy. J Korean Acad Fam Med 2004;25(10):713-20.
  21. Mihaylova B, Emberson J, Blackwell L, Keech A, Simes J, Barnes EH. The effects of lowering LDL cholesterol with statin therapy in people at low risk of vascular disease: Meta-analysis of individual data from 27 randomised trials. Lancet 2012;380(9841):581-90. https://doi.org/10.1016/S0140-6736(12)60367-5
  22. Park HJ, Zhang Y, Georgescu SP, Johnson KL, Kong D, Galper JB. Human Umbilical Vein Endothelial Cells and Human Dermal Microvascular Endothelial Cells Offer New Insights Into the Relationship Between Lipid Metabolism and Angiogenesis. Stem Cell Rev 2006;2(2):93-102. https://doi.org/10.1007/s12015-006-0015-x
  23. Han JM, Li H, Cho MH, Baek SH, Lee CH, Park HY, et al. Soy-Leaf Extract Exerts Atheroprotective Effects via Modulation of Kruppel-Like Factor 2 and Adhesion Molecules. Int J Mol Sci 2017;18(2):373. https://doi.org/10.3390/ijms18020373
  24. Christopherson K, Hromas R. Chemokine Regulation of Normal and Pathologic Immune Responses. Stem Cells 2001;19(5):388-96. https://doi.org/10.1634/stemcells.19-5-388
  25. Feinberg MW, Lin Z, Fisch S, Jain MK. An emerging role for Kruppel-like factors in vascular biology. Trends Cardiovasc Med 2004;14(6):241-6. https://doi.org/10.1016/j.tcm.2004.06.005
  26. Channon KM, George SE, Qian HS. Nitric Oxide Synthase in Atherosclerosis and Vascular Injury: insights from experimental gene therapy. Arterioscler Thromb Vasc Biol 2000;20(8):1873-81. https://doi.org/10.1161/01.ATV.20.8.1873
  27. Johnson GL, Lapadat R. Mitogen-Activated Protein Kinase Pathways Mediated by ERK, JNK, and p38 Protein Kinases. Science 2002;298(5600):1911-2. https://doi.org/10.1126/science.1072682
  28. Parravicini V, Gadina M, Kovarova M, Odom S, Gonzalez-Espinosa C, Furumoto Y, et al. Fyn kinase initiates complementary signals required for IgE-dependent mast cell degranulation. Nat Immunol 2002;3(8):741-8. https://doi.org/10.1038/ni817
  29. Cohen MP, Shea E, Chen S Shearman CW. Glycated albumin increases oxidative stress, activates NF-κB and extracellular signal-regulated kinase(ERK), and stimulates ERK-dependent transforming growth factor-β1 production in macrophage RAW cells. J Lab Clin Med 2003;141(4):242-9. https://doi.org/10.1067/mlc.2003.27
  30. Song HY, Regnier CH, Kirschning CJ, Goeddel DV, Rothe M. Tumor necrosis factor(TNF)-mediated kinases cascades: Bifurcation of Nuclear Factor-κB and c-Jun N terminal kinases(JNK/SARK) pathways at TNF receptor-assoiated factor 2. Proc Natl Acad Sci USA 1997;94(18):9792-6. https://doi.org/10.1073/pnas.94.18.9792
  31. Grynberg K, Ma FY, Nikolic-Paterson DJ. The JNK Signaling Pathway in Renal Fibrosis. Front Phys 2017;8:829. https://doi.org/10.3389/fphys.2017.00829
  32. Dean JLE, Brook M, Clark AR, Saklatvala J. p38 mitogen activated protein kinase regulates cycloxygenase-2 mRNA stability and transcription in lipopolysaccaride treated human monocytes. J Biol chem 1999;274(1):264-9. https://doi.org/10.1074/jbc.274.1.264
  33. Saklatvala J. The p38 MAP kinase pathway as a therapeutic target in inflammatory disease. Curr Opin Pharmacol 2004;4(4):372-7. https://doi.org/10.1016/j.coph.2004.03.009