대한상한금궤의학회지

Korean Medical Association of Clinical Sanghan-Geumgwe (대한상한금궤의학회)
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Effects of Red Koji-Fermented Bupleuri Radix Extracts on Lipopolysaccharide-Induced Rat Acute Lung Injury

홍국발효 시호(柴胡)가 Lipopolysaccharide로 유발된 급성 폐 손상에 미치는 영향

  • Seo, Young-ho (Seo-Young-Ho Korean Medicine Clinic) ;
  • Jung, Tae-young (Dept. of Diagnostics, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Jong-dea (Dept. of Internal Medicine, College of Korean Medicine, Daegu Haany University) ;
  • Choi, Hae-yun (Dept. of Internal Medicine, College of Korean Medicine, Daegu Haany University)
  • 서영호 (서영호한의원) ;
  • 정태영 (대구한의대학교 한의과대학 진단학교실) ;
  • 김종대 (대구한의대학교 한의과대학 내과학교실) ;
  • 최해윤 (대구한의대학교 한의과대학 내과학교실)
  • Received : 2021.12.02
  • Accepted : 2021.12.31
  • Published : 2021.12.31
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Abstract

Objective : This study aimed to assess the preventive effect of Bupleuri Radix aqueous extracts (BR) and red koji-fermented BR (fBR) in lipopolysaccharide (LPS)-induced acute lung injury in a rat model. Methods : Rats were administered 30, 60, or 120 mg/kg/day of fBR for 28 days before LPS treatments. All rats were sacrificed 5 h after LPS treatment (500 ㎍/head, intratracheal instillation). Body weights, lung weights, pulmonary transcapillary albumin transit, arterial gas parameters (pH, partial pressure [Pa] of O2, PaCO2), bronchoalveolar lavage fluid (BALF) protein, lactate dehydrogenase (LDH), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), total cell numbers, neutrophil/alveolar macrophage ratios, lung malondialdehyde (MDA), and myeloperoxidase (MPO) were measured. In addition, histopathological changes including the luminal surface of alveoli (LSA), thickness of alveolar septum, and number of polymorphonuclear neutrophils (PMNs) were checked. Results : LPS injection led to increases in lung weights, pulmonary transcapillary albumin transit, BALF protein, LDH, TNF-α and IL-1β contents, total cells, neutrophil and alveolar macrophage ratios, lung MDA, MPO, alveolar septum thickness, and PMNs, and decreases in PaCO2 and pH of arterial blood and LSA. However, these LPS-induced acute lung injuries were inhibited by pretreatment of 30, 60, and 120 mg/kg of fBR. The most favorable effects were seen with 30 mg/kg fBR as compared with 60 mg/kg of α-lipoic acid and BR. Conclusions : fBR showed preventive effects on LPS-induced acute lung injury, which resembles acute respiratory distress syndrome. The mechanisms of action were likely via antioxidant and anti-inflammatory means.

Keywords

References

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