Journal of Veterinary Science

  • 제22권2호
  • /
  • Pages.15.1-15.13
  • /
  • 2021
  • /
  • 1229-845X(pISSN)
  • /
  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지
DOI QR코드

DOI QR Code

Protective efficacy of attenuated Salmonella Typhimurium strain expressing BLS, Omp19, PrpA, or SOD of Brucella abortus in goats

  • Leya, Mwense (College of Veterinary Medicine, Jeonbuk National University) ;
  • Kim, Won Kyong (College of Veterinary Medicine, Jeonbuk National University) ;
  • Ochirkhuyag, Enkhsaikhan (College of Veterinary Medicine, Jeonbuk National University) ;
  • Yu, Eun-Chae (Korea Zoonosis Research institute, Jeonbuk National University) ;
  • Kim, Young-Jee (Korea Zoonosis Research institute, Jeonbuk National University) ;
  • Yeo, Yoonhwan (Korea Zoonosis Research institute, Jeonbuk National University) ;
  • Yang, Myeon-Sik (College of Veterinary Medicine, Jeonbuk National University) ;
  • Han, Sang-Seop (Korea Zoonosis Research institute, Jeonbuk National University) ;
  • Lee, John Hwa (College of Veterinary Medicine, Jeonbuk National University) ;
  • Tark, Dongseob (Korea Zoonosis Research institute, Jeonbuk National University) ;
  • Hur, Jin (College of Veterinary Medicine, Jeonbuk National University) ;
  • Kim, Bumseok (College of Veterinary Medicine, Jeonbuk National University)
  • 투고 : 2020.09.18
  • 심사 : 2021.01.04
  • 발행 : 2021.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Background: Attenuated Salmonella strain can be used as a vector to transport immunogens to the host antigen-binding sites. Objectives: The study aimed to determine the protective efficacy of attenuated Salmonella strain expressing highly conserved Brucella immunogens in goats. Methods: Goats were vaccinated with Salmonella vector expressing individually lipoprotein outer-membrane protein 19 (Omp19), Brucella lumazine synthase (BLS), proline racemase subunit A (PrpA), Cu/Zn superoxide dismutase (SOD) at 5 × 109 CFU/mL and challenge of all groups was done at 6 weeks after vaccination. Results: Among these vaccines inoculated at 5 × 109 CFU/mL in 1 mL, Omp19 or SOD showed significantly higher serum immunoglobulin G titers at (2, 4, and 6) weeks post-vaccination, compared to the vector control. Interferon-γ production in response to individual antigens was significantly higher in SOD, Omp19, PrpA, and BLS individual groups, compared to that in the vector control (all p < 0.05). Brucella colonization rate at 8 weeks post-challenge showed that most vaccine-treated groups exhibited significantly increased protection by demonstrating reduced numbers of Brucella in tissues collected from vaccinated groups. Real-time polymerase chain reaction revealed that Brucella antigen expression levels were reduced in the spleen, kidney, and parotid lymph node of vaccinated goats, compared to the non-vaccinated goats. Besides, treatment with vaccine expressing individual antigens ameliorated brucellosis-related histopathological lesions. Conclusions: These results delineated that BLS, Omp19, PrpA, and SOD proteins achieved a definite level of protection, indicating that Salmonella Typhimurium successfully delivered Brucella antigens, and that individual vaccines could differentially elicit an antigen-specific immune response.

키워드

과제정보

This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (Grant No.: HI16C2130) and supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2019R1A6A1A03033084).

참고문헌

  1. Poester FP, Samartino LE, Santos RL. Pathogenesis and pathobiology of brucellosis in livestock. Rev Sci Tech. 2013;32(1):105-115. https://doi.org/10.20506/rst.32.1.2193
  2. Spink W. The Evolution of the Concept that Brucellosis is a Disease of Animals and Man. The Nature of Brucellosis Minneapolis. Lund: Lund Press Inc.; 1956, 3-27.
  3. McDuff CR, Jones LM, Wilson JB. Characteristics of Brucella phage. J Bacteriol. 1962;83(2):324-329. https://doi.org/10.1128/jb.83.2.324-329.1962
  4. Payne JM. The pathogenesis of experimental brucellosis in the pregnant cow. J Pathol Bacteriol. 1959;78(2):447-463. https://doi.org/10.1002/path.1700780211
  5. Nicoletti P. The epidemiology of bovine brucellosis. Adv Vet Sci Comp Med. 1980;24:69-98.
  6. Oyewumi MO, Kumar A, Cui Z. Nano-microparticles as immune adjuvants: correlating particle sizes and the resultant immune responses. Expert Rev Vaccines. 2010;9(9):1095-1107. https://doi.org/10.1586/erv.10.89
  7. Stabel TJ, Mayfield JE, Morfitt DC, Wannemuehler MJ. Oral immunization of mice and swine with an attenuated Salmonella choleraesuis [delta cya-12 delta(crp-cdt)19] mutant containing a recombinant plasmid. Infect Immun. 1993;61(2):610-618. https://doi.org/10.1128/iai.61.2.610-618.1993
  8. Hur J, Lee JH. Enhancement of immune responses by an attenuated Salmonella enterica serovar Typhimurium strain secreting an Escherichia coli heat-labile enterotoxin B subunit protein as an adjuvant for a live Salmonella vaccine candidate. Clin Vaccine Immunol. 2011;18(2):203-209. https://doi.org/10.1128/CVI.00407-10
  9. Lalsiamthara J, Lee JH. Brucella lipopolysaccharide reinforced Salmonella delivering Brucella immunogens protects mice against virulent challenge. Vet Microbiol. 2017;205:84-91. https://doi.org/10.1016/j.vetmic.2017.05.012
  10. Kim WK, Moon JY, Kim S, Hur J. Comparison between immunization routes of live attenuated Salmonella Typhimurium strains expressing BCSP31, Omp3b, and SOD of Brucella abortus in murine model. Front Microbiol. 2016;7:550. https://doi.org/10.3389/fmicb.2016.00550
  11. Ackermann MR, Cheville NF, Deyoe BL. Bovine ileal dome lymphoepithelial cells: endocytosis and transport of Brucella abortus strain 19. Vet Pathol. 1988;25(1):28-35. https://doi.org/10.1177/030098588802500104
  12. Paixao TA, Roux CM, den Hartigh AB, Sankaran-Walters S, Dandekar S, Santos RL, et al. Establishment of systemic Brucella melitensis infection through the digestive tract requires urease, the type IV secretion system, and lipopolysaccharide O antigen. Infect Immun. 2009;77(10):4197-4208. https://doi.org/10.1128/IAI.00417-09
  13. Hong CB, Donahue JM, Giles RC Jr, Poonacha KB, Tuttle PA, Cheville NF. Brucella abortus-associated meningitis in aborted bovine fetuses. Vet Pathol. 1991;28(6):492-496. https://doi.org/10.1177/030098589102800605
  14. Baldwin CL, Winter AJ. Macrophages and Brucella. Immunol Ser. 1994;60:363-380.
  15. Liautard JP, Gross A, Dornand J, Kohler S. Interactions between professional phagocytes and Brucella spp. Microbiologia 1996;12(2):197-206.
  16. Skendros P, Boura P. Immunity to brucellosis. Rev Sci Tech. 2013;32(1):137-147. https://doi.org/10.20506/rst.32.1.2190
  17. Velikovsky CA, Cassataro J, Giambartolomei GH, Goldbaum FA, Estein S, Bowden RA, et al. A DNA vaccine encoding lumazine synthase from Brucella abortus induces protective immunity in BALB/c mice. Infect Immun. 2002;70(5):2507-2511. https://doi.org/10.1128/iai.70.5.2507-2511.2002
  18. Spera JM, Ugalde JE, Mucci J, Comerci DJ, Ugalde RA. A B lymphocyte mitogen is a Brucella abortus virulence factor required for persistent infection. Proc Natl Acad Sci U S A. 2006;103(44):16514-16519. https://doi.org/10.1073/pnas.0603362103
  19. Pasquevich KA, Estein SM, Garcia Samartino C, Zwerdling A, Coria LM, Barrionuevo P, et al. Immunization with recombinant Brucella species outer membrane protein Omp16 or Omp19 in adjuvant induces specific CD4+ and CD8+ T cells as well as systemic and oral protection against Brucella abortus infection. Infect Immun. 2009;77(1):436-445. https://doi.org/10.1128/IAI.01151-08
  20. Cloeckaert A, Vizcaino N, Paquet JY, Bowden RA, Elzer PH. Major outer membrane proteins of Brucella spp.: past, present and future. Vet Microbiol. 2002;90(1-4):229-247. https://doi.org/10.1016/S0378-1135(02)00211-0
  21. Onate AA, Vemulapalli R, Andrews E, Schurig GG, Boyle S, Folch H. Vaccination with live Escherichia coli expressing Brucella abortus Cu/Zn superoxide dismutase protects mice against virulent B. abortus. Infect Immun. 1999;67(2):986-988. https://doi.org/10.1128/iai.67.2.986-988.1999
  22. Tabatabai LB, Pugh GW Jr. Modulation of immune responses in Balb/c mice vaccinated with Brucella abortus Cu-Zn superoxide dismutase synthetic peptide vaccine. Vaccine. 1994;12(10):919-924. https://doi.org/10.1016/0264-410X(94)90035-3
  23. Lee JJ, Lim JJ, Kim DG, Simborio HL, Kim DH, Reyes AW, et al. Characterization of culture supernatant proteins from Brucella abortus and its protection effects against murine brucellosis. Comp Immunol Microbiol Infect Dis. 2014;37(4):221-228. https://doi.org/10.1016/j.cimid.2014.06.001
  24. Lalsiamthara J, Kamble NM, Lee JH. A live attenuated Salmonella Enteritidis secreting detoxified heat labile toxin enhances mucosal immunity and confers protection against wild-type challenge in chickens. Vet Res (Faisalabad). 2016;47(1):60. https://doi.org/10.1186/s13567-016-0348-7
  25. Neta AVC, Mol JP, Xavier MN, Paixao TA, Lage AP, Santos RL. Pathogenesis of bovine brucellosis. Vet J. 2010;184(2):146-155. https://doi.org/10.1016/j.tvjl.2009.04.010
  26. Corbel MJ. Brucellosis in humans and animals: World Health Organization; 2006.
  27. Dorneles EM, Sriranganathan N, Lage AP. Recent advances in Brucella abortus vaccines. Vet Res (Faisalabad). 2015;46(1):76. https://doi.org/10.1186/s13567-015-0199-7
  28. Leya M, Kim WK, Cho JS, Yu EC, Kim YJ, Yeo Y, et al. Vaccination of goats with a combination Salmonella vector expressing four Brucella antigens (BLS, PrpA, Omp19, and SOD) confers protection against Brucella abortus infection. J Vet Sci. 2018;19(5):643-652. https://doi.org/10.4142/jvs.2018.19.5.643
  29. Lalsiamthara J, Won G, Lee JH. Effect of immunization routes and protective efficacy of Brucella antigens delivered via Salmonella vector vaccine. J Vet Sci. 2018;19(3):416-425. https://doi.org/10.4142/jvs.2018.19.3.416
  30. Gomez G, Adams LG, Rice-Ficht A, Ficht TA. Host-Brucella interactions and the Brucella genome as tools for subunit antigen discovery and immunization against brucellosis. Front Cell Infect Microbiol. 2013;3:17.
  31. Skendros P, Pappas G, Boura P. Cell-mediated immunity in human brucellosis. Microbes Infect. 2011;13(2):134-142. https://doi.org/10.1016/j.micinf.2010.10.015
  32. Yingst S, Hoover DL. T cell immunity to brucellosis. Crit Rev Microbiol. 2003;29(4):313-331. https://doi.org/10.1080/713608012
  33. Baldwin CL, Goenka R. Host immune responses to the intracellular bacteria Brucella: does the bacteria instruct the host to facilitate chronic infection? Crit Rev Immunol. 2006;26(5):407-442. https://doi.org/10.1615/CritRevImmunol.v26.i5.30