Journal of Veterinary Science

  • 제22권5호
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  • Pages.62.1-62.13
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  • 2021
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Metastasis prognostic factors and cancer stem cell-related transcription factors associated with metastasis induction in canine metastatic mammary gland tumors

  • Kim, Saetbyul (Department of Veterinary Obstetrics, College of Veterinary Medicine, Gyeongsang National University) ;
  • Bok, Eunyeong (Department of Veterinary Obstetrics, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Sangyeob (Department of Veterinary Obstetrics, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hyeon-Jeong (Department of Veterinary Obstetrics, College of Veterinary Medicine, Gyeongsang National University) ;
  • Choe, Yongho (Department of Veterinary Obstetrics, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Na-Hyun (Gyeongnam Department of Environment & Toxicology, Korea Institute of Toxicology) ;
  • Lee, Won-Jae (Department of Veterinary Obstetrics, College of Veterinary Medicine, Kyungpook National University) ;
  • Rho, Gyu-Jin (Department of Veterinary Obstetrics, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Sung-Lim (Department of Veterinary Obstetrics, College of Veterinary Medicine, Gyeongsang National University)
  • 투고 : 2021.05.17
  • 심사 : 2021.07.05
  • 발행 : 2021.09.30
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초록

Background: Canine mammary gland tumor (MGT) is the most common cancer in aged female dogs. Although it's important to identify reliable metastasis or prognostic factors by evaluating related to cell division, adhesion, and cancer stem cell-related transcription factor (TF) in metastasis-induced canine MGT, but there are limited studies. Objectives: We aimed to identify metastasis prognostic factors and cancer stem cell-TFs in canine MGTs. Methods: Age-matched female dogs diagnosed with MGT only were classified into metastatic and non-metastatic groups by histopathological staining of MGT tissues. The mRNA levels of cancer prognostic metastasis molecular factors (E-cadherin, ICAM-1, PRR14, VEGF, HPRT1, RPL4 and hnRNP H) and cancer stem cell-related TFs (Oct4, Sox2, and Nanog) were compared between metastatic and non-metastatic canine MGT tissues using qRT-PCR analysis. Results: The mRNA levels of ICAM-1, PRR14, VEGF, hnRNP H, Oct4, Sox2, and Nanog in metastatic MGT group were significantly higher than those in non-metastatic MGT group. However, mRNA level of RPL4 was significantly lower in metastatic MGT group. Loss of E-cadherin and HPRT1 was observed in the metastatic MGT group but it was not significant. Conclusions: Consistent expression patterns of all metastasis-related factors showing elevation in ICAM-1, PRR14, VEGF, hnRNP H, Oct4, Sox2, and Nanog, but decreases in RPL4 levels occurred in canine MGT tissues, which was associated with metastasis. Thus, these cancer prognostic metastasis factors and TFs of cancer stem cells, except for E-cadherin and HPRT1, can be used as reliable metastasis factors for canine MGT and therapeutic strategy.

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과제정보

This study was supported by a grant from the National Research Foundation (NRF) of Korea, funded by the government of the Republic of Korea (NRF-2021R1A2C1007054).

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