Journal of Veterinary Science

  • 제22권6호
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  • Pages.86.1-86.13
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  • 2021
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Conditioned medium of E17 rat brain cells induced differentiation of primary colony of mice blastocyst into neuron-like cells

  • Budiariati, Vista (Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada) ;
  • Rinendyaputri, Ratih (Center for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health Republic of Indonesia) ;
  • Noviantari, Ariyani (Center for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health Republic of Indonesia) ;
  • Haq, Noer Muhammad Dliyaul (Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, IPB University) ;
  • Budiono, Dwi (Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, IPB University) ;
  • Pristihadi, Diah Nugrahani (Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, IPB University) ;
  • Juliandi, Berry (Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University) ;
  • Fahrudin, Mokhamad (Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, IPB University) ;
  • Boediono, Arief (Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, IPB University)
  • 투고 : 2021.05.11
  • 심사 : 2021.08.24
  • 발행 : 2021.11.30
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초록

Background: Conditioned medium is the medium obtained from certain cultured cells and contained secretome from the cells. The secretome, which can be in the form of growth factors, cytokines, exosomes, or other proteins secreted by the cells, can induce the differentiation of cells that still have pluripotent or multipotent properties. Objectives: This study examined the effects of conditioned medium derived from E17 rat brain cells on cells with pluripotent properties. Methods: The conditioned medium used in this study originated from E17 rat brain cells. The CM was used to induce the differentiation of primary colonies of mice blastocysts. Primary colonies were stained with alkaline phosphatase to analyze the pluripotency. The morphological changes in the colonies were examined, and the colonies were stained with GFAP and Neu-N markers on days two and seven after adding the conditioned medium. Results: The conditioned medium could differentiate the primary colony, beginning with the formation of embryoid-body-like structure; round GFAP positive cells were identified. Finally, neuron-like cells testing positive for Neu-N were observed on the seventh day after adding the conditioned medium. Conclusions: Conditioned medium from different species, in this case, E17 rat brain cells, induced and promoted the differentiation of the primary colony from mice blastocysts into neuron-like cells. The addition of CM mediated neurite growth in the differentiation process.

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과제정보

The authors would like to thank the Directorate of Research and Community Service, Directorate General of Higher Education, Ministry of Research, Technology, and Higher Education, the Republic of Indonesia for the research funding through the PMDSU grant (No. 1521/IT3.11/PN/2018) and the National Institute of Health Research and Development, Ministry of Health, Indonesia for the supports and laboratory facilities.

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