Journal of Veterinary Science

  • 제22권4호
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  • Pages.42.1-42.16
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  • 2021
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific-pathogen-free chicken

  • Sabarudin, Nur Syazana (Laboratory of Vaccine and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia) ;
  • Tan, Sheau Wei (Laboratory of Vaccine and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia) ;
  • Phang, Yuen Fun (Regional Operating Unit South East Asia and South Korea, Boehringer Ingelheim Singapore Pte Ltd) ;
  • Omar, Abdul Rahman (Laboratory of Vaccine and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia)
  • 투고 : 2021.02.04
  • 심사 : 2021.04.15
  • 발행 : 2021.07.31
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초록

Background: Inclusion body hepatitis (IBH) is an economically important viral disease primarily affecting broiler and breeder chickens. All 12 serotypes of fowl adenovirus (FAdV) can cause IBH. Objectives: To characterize FAdV isolates based on phylogenetic analysis, and to study the pathogenicity of FAdV-8b in specific-pathogen-free (SPF) chickens following virus inoculation via oral and intramuscular (IM) routes. Methods: Suspected organ samples were subjected to virus isolation and polymerase chain reaction (PCR) for FAdV detection. Hexon gene sequencing and phylogenetic analysis were performed on FAdV-positive samples for serotype identification. One FAdV-8b isolate, UPM/FAdV/420/2017, was selected for fiber gene characterization and pathogenicity study and was inoculated in SPF chickens via oral and IM routes. Results: The hexon gene phylogenetic analysis revealed that all isolates belonged to FAdV-8b. The fiber gene-based phylogenetic analysis of isolate UPM/FAdV/420/2017 supported the grouping of that isolate into FAdV species E. Pathogenicity study revealed that, chickens infected with UPM/FAdV/420/2017 via the IM route had higher clinical score values, higher percent mortality, higher degree of the liver lesions, higher antibody response (p < 0.05), and higher virus shedding amounts (p < 0.05) than those infected via the oral route. The highest virus copy numbers were detected in liver and gizzard. Conclusions: FAdV-8b is the dominant FAdV serotype in Malaysia, and pathogenicity study of the FAdV-8b isolate UPM/FAdV/420/2017 indicated its ability to induce IBH in young SPF chickens when infected via oral or IM routes.

키워드

과제정보

The authors would like to thank Dr. Siti Nor Azizah Mahamud for assistance with the experimental animal procedures and other staff of the Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia for their helpfulness in the collection of FAdV isolates.

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