Journal of Veterinary Science

  • 제22권5호
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  • Pages.75.1-75.10
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  • 2021
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Evaluation of circulating PD-1 and PD-L1 as diagnostic biomarkers in dogs with tumors

  • Song, Doo-Won (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University) ;
  • Ro, Woong-Bin (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University) ;
  • Park, Hee-Myung (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University)
  • 투고 : 2021.04.08
  • 심사 : 2021.08.24
  • 발행 : 2021.09.30
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초록

Background: Programmed cell death protein-1 (PD-1) and programmed cell death ligand-1 (PD-L1) have important roles in tumor evasion of the immune system. Objectives: This study aimed to assess the diagnostic utility of circulating PD-1 and PD-L1 levels in healthy dogs and dogs with tumors. Methods: Circulating PD-1 and PD-L1 levels in the serum of 71 dogs with tumors were compared with those of 52 healthy dogs by performing enzyme-linked immunosorbent assay (ELISA). Results: The ELISA results revealed higher circulating PD-1 and PD-L1 levels in dogs with tumors (2.9 [2.2-3.7] ng/mL; median [IQR] and 2.4 [1.4-4.4] ng/mL, respectively) than in healthy dogs (2.4 [1.9-3.0] ng/mL; p = 0.012 and 1.4 [0.9-2.1] ng/mL; p < 0.001, respectively). Especially, there was a significant difference in circulating PD-1 levels between healthy dogs and dogs with malignant epithelial tumors (2.4 [1.9-3.0] ng/mL and 3.1 [2.6-4.4] ng/mL, respectively; p < 0.01). In addition, there was a significant difference in circulating PD-L1 levels between healthy dogs and dogs with lymphomas (1.4 [0.9-2.1] ng/mL and 2.7 [1.6-5.8] ng/mL, respectively; p < 0.001). Conclusion: This study indicates that circulating PD-1 and PD-L1 have potential as tumor diagnostic biomarkers in dogs with tumors.

키워드

과제정보

We thank members of our laboratory for collecting the samples used in this study, and thank See-Hyoung Park for advice and BioSharp for technical assistance. The study results represent a portion of the Ph.D. thesis by Doo-Won Song.

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