International Journal of Oral Biology

  • 제46권1호
  • /
  • Pages.39-44
  • /
  • 2021
  • /
  • 1226-7155(pISSN)
  • /
  • 2287-6618(eISSN)
대한구강생물학회 (The Korean Academy of Oral Biology)
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Expression of neurotransmitter receptors in oral keratinocytes and their response to agonists

  • Choi, Eun Ji (Department of Oral Microbiology and Immunology, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Chang, Sung-Ho (Department of Oral Microbiology and Immunology, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Choi, Se-Young (Department of Neuroscience, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Choi, Youngnim (Department of Oral Microbiology and Immunology, School of Dentistry and Dental Research Institute, Seoul National University)
  • 투고 : 2021.01.21
  • 심사 : 2021.03.19
  • 발행 : 2021.03.31
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초록

This study aimed to investigate whether neurotransmitter receptors in the nervous system were also expressed in oral keratinocytes. Expressions of various neurotransmitter receptor genes in immortalized mouse oral keratinocyte (IMOK) cells were examined by reverse transcriptase polymerase chain reaction. IMOK cells expressed calcitonin gene-related peptide (CGRP) receptor subunit genes Ramp1 and Ramp3 and glutamate receptor subunit genes Grina, Gria3, Grin1, Grin2a, and Grin2d. Moreover, IMOK cells expressed Adrb2 and Chrna5 that encode beta 2 adrenergic receptor and cholinergic receptor nicotinic alpha 5 for sympathetic and parasympathetic neurotransmitters, respectively. The expression of Bdkrb1 and Ptger4, which encode receptors for bradykinin and prostaglandin E2 involved in inflammatory responses, was also observed at low levels. Expressions of Ramp1 and Grina in the mouse gingival epithelium were also confirmed by immunohistochemistry. When the function of neurotransmitter receptors expressed on IMOK cells was tested by intracellular calcium response, CGRP, glutamate, and cholinergic receptors did not respond to their agonists, but the bradykinin receptor responded to bradykinin. Collectively, oral keratinocytes express several neurotransmitter receptors, suggesting the potential regulation of oral epithelial homeostasis by the nervous system.

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