International Journal of Oral Biology

  • 제46권1호
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  • Pages.15-22
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  • 2021
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  • 1226-7155(pISSN)
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  • 2287-6618(eISSN)
대한구강생물학회 (The Korean Academy of Oral Biology)
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Alpha-lipoic acid protects human dopaminergic neuronal cells against hydrogen peroxide-induced cell injury by inhibiting autophagy and apoptosis

  • Kang, Kyeong-Rok (The Institute of Dental Science, Chosun University) ;
  • Kim, Jae-Sung (The Institute of Dental Science, Chosun University) ;
  • Kim, Tae-Hyeon (The Institute of Dental Science, Chosun University) ;
  • Seo, Jeong-Yeon (Department of Integrative Biological Sciences & BK21 FOUR Educational Research Group for Age-associated Disorder Control Technology, Chosun University) ;
  • Lim, HyangI (The Institute of Dental Science, Chosun University) ;
  • Park, Jong-Hyun (The Institute of Dental Science, Chosun University) ;
  • Yang, Kwang Yeol (The Institute of Dental Science, Chosun University) ;
  • Yu, Sun-Kyoung (The Institute of Dental Science, Chosun University) ;
  • Kim, Heung-Joong (The Institute of Dental Science, Chosun University) ;
  • Kim, Chun Sung (The Institute of Dental Science, Chosun University) ;
  • Chun, Hong Sung (Department of Integrative Biological Sciences & BK21 FOUR Educational Research Group for Age-associated Disorder Control Technology, Chosun University) ;
  • Lee, Dong-Seol (Laboratory for the Study of Regenerative Dental Medicine, Department of Oral Histology-Developmental Biology, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Park, Joo-Cheol (Laboratory for the Study of Regenerative Dental Medicine, Department of Oral Histology-Developmental Biology, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Kim, Do Kyung (The Institute of Dental Science, Chosun University)
  • 투고 : 2021.02.24
  • 심사 : 2021.03.10
  • 발행 : 2021.03.31
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초록

Alpha-lipoic acid (ALA) is a naturally occurring antioxidant and has been previously used to treat diabetes and cardiovascular disease. However, the autophagy effects of ALA against oxidative stress-induced dopaminergic neuronal cell injury remain unclear. The aim of this study was to investigate the role of ALA in autophagy and apoptosis against oxidative stress in the SH-SY5Y human dopaminergic neuronal cell line. We examined SH-SY5Y phenotypes using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay (cell viability/proliferation), 4′,6-diamidino-2-phenylindole dihydrochloride nuclear staining, Live/Dead cell assay, cellular reactive oxygen species (ROS) assay, immunoblotting, and immunocytochemistry. Our data showed ALA attenuated hydrogen peroxide (H2O2)-induced ROS generation and cell death. ALA effectively suppressed Bax up-regulation and Bcl-2 and Bcl-xL down-regulation. Furthermore, ALA increased the expression of the antioxidant enzyme, heme oxygenase-1. Moreover, the expression of Beclin-1 and LC-3 autophagy biomarkers was decreased by ALA in our cell model. Combined, these data suggest ALA protects human dopaminergic neuronal cells against H2O2-induced cell injury by inhibiting autophagy and apoptosis.

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참고문헌

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