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Simultaneous determination of saikosaponin derivatives in Bupleurum falcatum by HPLC-ELSD analysis using different extraction methods

  • Choi, Jungwon (Department of Plant Science and Technology, Chung-Ang University) ;
  • Kim, Juree (Department of Plant Science and Technology, Chung-Ang University) ;
  • Kang, Sam Sik (Natural Products Research Institute and College of Pharmacy, Seoul National University) ;
  • Lee, Sanghyun (Department of Plant Science and Technology, Chung-Ang University)
  • Received : 2021.01.05
  • Accepted : 2021.01.15
  • Published : 2021.03.31

Abstract

Saikosaponin derivatives such as saikosaponins A, B1, B2, B3, B4, C, and D present in Bupleurum falcatum were analyzed by a high performance liquid chromatograph equipped with an evaporative light scattering detector, using different extraction solvents (water and 70% ethanol). The samples were injected into a YMC Pack Pro C18 column and separated using a gradient elution system with a mobile phase composed of acetonitrile and water at a flow rate of 1.1 mL/min. The content of saikosaponin derivatives was higher in 70% ethanol extract than in water extract. This study provides an efficient analytical method for determining the optimal conditions for extraction of saikosaponin derivatives, which can be used as a basis for development of functional foods and pharmaceutical products from B. falcatum.

Keywords

References

  1. Sun XB, Matsumoto T, Yamada H (1991) Effects of a polysaccharide fraction from the roots of Bupleurum falcatum L. on experimental gastric ulcer models in rats and mice. J Pharm Pharmacol 43: 699-704 https://doi.org/10.1111/j.2042-7158.1991.tb03461.x
  2. Yao RU, Zou YF, Chen XF (2013) Traditional use, pharmacology, toxicology, and quality control of species in genus Bupleurum L. Chin Herb Med 5: 245-255 https://doi.org/10.1016/s1674-6384(13)60036-2
  3. Kim MS, Kwon DY, Cho HJ, Lee MS (2006) Protective effects of Korean herbal remedy against oxidative stress in cardiomyocytes. Phytother Res 20: 235-236 https://doi.org/10.1002/ptr.1837
  4. Lin CC, Chiu HF, Yen MH, Wu CC, Chen MF (1990) The pharmacological and pathological studies on Taiwan folk medicine (III): The effects of Bupleurum kaoi and cultivated Bupleurum falcatum var. komarowi. Am J Chin Med 18: 105-112 https://doi.org/10.1142/S0192415X90000149
  5. Shimaoka A, Seo S, Minato H (1975) Saponins isolated from Bupleurum falcatum L.; components of saikosaponin B. J Chem Soc 20: 2043-2048
  6. Yamada H, Ra KS, Kiyohara H, Cyong JC, Otsuka Y (1989) Structural characterization of an anti-complementary pectic polysaccharide from the roots of Bupleurum falcatum L. Carbohydr Res 189: 209-226 https://doi.org/10.1016/0008-6215(89)84098-4
  7. Ebata N, Nakajima K, Hayashi K, Okada M, Maruno M (1996) Saponins from the root of Bupleurum falcatum. Phytochemistry 41: 895-901 https://doi.org/10.1016/0031-9422(95)00720-2
  8. Ono M, Yoshida A, Ito Y, Nohara T (1999) Phenethyl alcohol glycosides and isopentenol glycoside from fruit of Bupleurum falcatum. Phytochemistry 51: 819-823 https://doi.org/10.1016/S0031-9422(99)00073-4
  9. Zong ZP, Yamamoto KF, Ota T, Guan X, Murakami M, Li AL, Yamaguchi N, Tanino M, Odashima S (1998) Saikosaponin B2 induces differentiation without growth inhibition in cultured B16 melanoma cells. Cell Struct Funct 23: 265-272 https://doi.org/10.1247/csf.23.265
  10. Chen JC, Chang NW, Chung JG, Chen KC (2003) Saikosaponin-A induces apoptotic mechanism in human breast MDA-MB-231 and MCF-7 cancer cells. J Chin Med 31: 363-377 https://doi.org/10.1142/S0192415X03001065
  11. Shyu K, Tsai S, Wang B, Liu Y, Lee C (2004) Saikosaponin C induces endothelial cells growth, migration and capillary tube formation. Life Sci 76: 813-826 https://doi.org/10.1016/j.lfs.2004.10.003
  12. Zhu J, Luo C, Wang P, He Q, Zhou J, Peng H (2013) Saikosaponin A mediates the inflammatory response by inhibiting the MAPK and NF-κB pathways in LPS-stimulated RAW 264.7 cells. Exp Ther Med 5: 1345-1350 https://doi.org/10.3892/etm.2013.988
  13. Shibata S, Kitagawa I, Fujimoto H (1966) The chemical studies on oriental plant drugs. XV. On the constituents of Bupleurum spp. (2). The structure of saikogenin A, a sapogenin of Bupleurum falcatum L. Chem Pharm Bull 14: 1023-1033 https://doi.org/10.1248/cpb.14.1023
  14. Yuan B, Yang R, Ma Y, Zhou S, Zhang X, Liu Y (2017) A systematic review of the active saikosaponins and extracts isolated from Radix Bupleuri and their applications. Pharma Boil 55: 620-635 https://doi.org/10.1080/13880209.2016.1262433
  15. Ran X, Gia C, Seiichi PT (2004) On the origins of triterpenoid skeletal diversity. Phytochemistry 65: 261-291 https://doi.org/10.1016/j.phytochem.2003.11.014
  16. Sun Y, Cai TT, Zhou XB, Xu Q (2009) Saikosaponin a inhibits the proliferation and activation of T cells through cell cycle arrest and induction of apoptosis. Int Immunopharmacol 9: 978-983 https://doi.org/10.1016/j.intimp.2009.04.006
  17. Yamada H, Hirano M, Kiyohara H (1991) Partial structure of an anti-ulcer pectic polysaccharide from the roots of Bupleurum falcatum L. Carbohydr Res 219: 173-192 https://doi.org/10.1016/0008-6215(91)89050-P
  18. Feng L, Liu L, Zhao Y, Zhao R (2017) Saikosaponins A, C and D enhance liver-targeting effects of anticancer drugs by modulating drug transporters. Oncotarget 8: 110092-110102 https://doi.org/10.18632/oncotarget.22639
  19. Park KH, Park J, Koh D, Lim Y (2002) Effect of saikosaponin-A, a triterpenoid glycoside, isolated from Bupleurum falcatum on experimental allergic asthma. Phytother Res 16: 359-363 https://doi.org/10.1002/ptr.903
  20. Leung CY, Liu L, Wong RNS, Zeng YY, Li M, Zhou H (2005) Saikosaponin-D inhibits T cell activation through the modulation of PKCO, JNK, and NF-κB transcription factor. Biochem Biophys Res Commun 338: 1920-1927 https://doi.org/10.1016/j.bbrc.2005.10.175
  21. Takagi K, Shibata M (1969) Pharmacological studies on Bupleurum falcatum L. acute toxicity and central depressant action of crude saikosides. Yakugaku Zasshi 89: 712-720 https://doi.org/10.1248/yakushi1947.89.5_712
  22. Park WH, Kang S, Piao Y, Pak CJ, Oh MS, Kim J, Kang MS, Pak YK (2015) Ethanol extract of Bupleurum falcatum and saikosaponins inhibit neuroinflammation via inhibition of NF-κB. J Ethnopharmacol 4: 37-44
  23. Lee JH (2009) Simultaneous determination of saikosaponins in Bupleuri Radix by high performance liquid chromatography evaporative light scattering detection and liquid chromatography - mass spectrometry. Dissertation, Chung-Ang University