DOI QR코드

DOI QR Code

Development of HPLC Method for Quality Assessment of Marker Components in Atractylodis Rhizoma Alba

백출(Atractylodis Rhizoma Alba)의 품질평가를 위한 지표성분 분석법 평가

  • Lee, Jae-Woong (Medicinal Crops Seed Supply Center, National Development Institute of Korean Medicine) ;
  • Kim, Joon-Hee (Medicinal Crops Seed Supply Center, National Development Institute of Korean Medicine) ;
  • Kang, Byoung-Man (Medicinal Crops Seed Supply Center, National Development Institute of Korean Medicine) ;
  • Ahn, Byung-Kwan (Medicinal Crops Seed Supply Center, National Development Institute of Korean Medicine)
  • 이재웅 (한국한의약진흥원 한약자원개발본부 약용작물종자보급센터) ;
  • 김준희 (한국한의약진흥원 한약자원개발본부 약용작물종자보급센터) ;
  • 강병만 (한국한의약진흥원 한약자원개발본부 약용작물종자보급센터) ;
  • 안병관 (한국한의약진흥원 한약자원개발본부 약용작물종자보급센터)
  • Received : 2020.07.27
  • Accepted : 2020.11.05
  • Published : 2021.02.01

Abstract

Homogenization of quality was important in order to use herbal medicines as pharmaceuticals. To solve this problem, it was important to establish quality standards. Atractylodis Rhizoma Alba has no quantitative method in the Korean Pharmacopoeia. Thus, we have researched to improve the quality evaluation method of Atractylodis Rhizoma Alba with an HPLC. Atractylenolide III and atracylodin were selected as potential marker compounds. This analytical procedure was subject to validation. According to validation guideline of South Korea's Ministry of Food and Drugs Safety, the specificity, linearity, precision, range, quantitative limits, detection limits and accuracy were measured. Because the specificity, linearity, precision, range, quantitative limits, detection limits and accuracy meet criteria of the guideline, the analytic method was validated. With this analysis, Atractylodis Rhizoma Alba and Atractylodis Rhizoma analyzed. As a result, both atractylenolide III and atracylodin appear to be suitable standard compounds. it confirmed that tractylodes Rhizome, similar to Atractylodes Rhizome Alba, could be distinguished.

백출의 기원 감별 및 품질기준 설정을 위하여 atractylenolide III과 atracylodin을 지표성분으로 설정하였다. HPLC를 이용하여 선정된 지표물질인 atractylenolide III과 atracylodin의 특이성, 직선성, 범위, 검출한계, 정량한계, 정확성, 정밀성에 대한 평가를 실시한 결과 모두 양호한 결과가 나타나 분석법에 문제는 없었으며, 백출과 창출을 각각 분석한 결과 위품인 창출에서만 atracylodin이 검출이 되어 위품 구별이 가능한 지표물질로 손색이 없었다.

Keywords

References

  1. Dong, H., L. He, M. Huang and Y. Dong. 2008. Anti-inflammatory components isolated from Atractylodes macrocephala Koidz. Nat. Prod. Res. 22(16):1418-27 (GBR). https://doi.org/10.1080/14786410801931629
  2. Jerun, U. 2015. Comparative study on efficacy of Atractylodes rhizome recorded in traditional medical literature and in recent pharmacological article. Korean Herb. Med. Inf. 3(2):1-16 (in Korean).
  3. Kim, S.Y., O.H. Kwon, J.H. Cho and J.H. Lim. 2001. Effects of flower removal on growth and content of essential oil in Atractylodes macrocephala Koidz. Korean J. Plant Res. 14(2):152-156 (in Korean).
  4. Lee, J., Y. Kim, S. Hong and C. Kim. 2002. Studies of taxonomic origins of Atractylodis rhizoma Alba and Atractylodis rhizoma. Korean Journal of Oriental Medicine 8:55-63 (in Korean).
  5. Lee, S.I. 1981. Studies on the diuretic action of Oryeongsan and Kami - Oryeongsan. Korean J. Pharmacogn. 12:31-43 (in Korean).
  6. National Institute of Food and Drug Safety Evaluation. 2010. Guidelines for setting the ingredient profile of herbal medicines. Cheongju, Korea. pp. 5-15 (in Korean).
  7. National Institute of Food and Drug Safety Evaluation. 2015. Test methods such as pharmaceuticals validation guidelines. Cheongju, Korea. pp. 5-15 (in Korean).
  8. National Institute of Food and Drug Safety Evaluation. 2017. Discrimination of atractylodes rhizome white. Cheongju, Korea. pp. 44-48 (in Korean).
  9. National Institute of Food and Drug Safety Evaluation. 2019. Guidelines for writing submission materials for recognition functional raw material. Cheongju, Korea. pp. 21-47 (in Korean).
  10. Nishikawa, Y., T. Seto, Y. Watanabe and I. Yasuda. 1977. Studies on the components of atractylodes III. new sesquiterpenoid in the rhizome of Atractylodes japonica Koidzumi. Yakugaku Zasshi 97:515-518 (in Japanese). https://doi.org/10.1248/yakushi1947.97.5_515
  11. The Hong Kong Department of Health. 2019. Chinese materia medica standards volume 3. Hong Kong, China. p. 273 (in Chinese).
  12. Yim, D.S., S.C. Yu and H.J. Chi. 1988. Phytochemical study on the rhizome of Atractylodes japonica from Korea. Korean J. Pharmacogn. 19(4):228-232 (in Korean).
  13. Yoon, J.H. and Y.S. Ju. 2018. A study on identification keys of Araliae continentalis Radix and its adulterants: Focused on external·internal morphology and pattern analysis. Korean J. Herbol. 33(2):29-43 (in Korean).
  14. Yosioka, I., T. Tani, M. Hirose and I. Kitagawa. 1974. Diacetylactractylodiol, a new acetylenic compound from Atractylodes japonica Koidzumi. Chem. Pharm. Bull. 22(8):1943-1945 (in Japanese). https://doi.org/10.1248/cpb.22.1943
  15. Yun, B.R., J.B. Weon, B. Lee, J. Lee, M.R. Eom and C.J. Ma. 2013. Quantitative analysis of atractylenolides I and III in Atractylodes japonica. Korean J. Pharmacogn. 44(1):53-59 (in Korean).