The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Species Diversity and Antifungal Activity of Endophytic Fungi Isolated from Angelica gigas Nakai

참당귀에서 분리된 내생균의 다양성과 병원균 억제 효과

  • Park, Hyeok (Department of Biology Education, Korea National University of Education) ;
  • Jung, Chung Ryul (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 박혁 (한국교원대학교 생물교육과) ;
  • 정충렬 (국립산림과학원 산림약용자원연구소) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2021.11.16
  • Accepted : 2021.12.15
  • Published : 2021.12.31
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Abstract

Endophytic fungal strains were isolated from leaves, stems, and roots of Angelica gigas. The fungal strains were identified based on their morphological characteristics and molecular analysis of the internal transcribed spacer (ITS). A total 35 species of endophytic fungi were identified. The diversity between the endophytic fungal communities differed depending on the tissues of A. gigas. The isolated endophytic fungi were screened for antifungal activity against a pathogenic fungus, Phoma sp. Y11, using a dual culture method. Fourteen species of endophytic fungi showed the standout inhibition effect against the Y11 strain. The results suggest that the endophytic fungi isolated from A. gigas could be used as a biological control agent against leaf spot disease of A. gigas.

다양한 지역에서 채취한 참당귀(Angelica gigas Nakai)의 잎, 줄기, 뿌리에서 내생균을 분리하였다. 내생균 균주는 형태적특징과 ITS (internal transcribed spacer) 지역의 염기서열을 이용하여 동정하였다. 총 35종의 내생균이 동정되었다. 내생균의 다양성은 참당귀의 조직에 따라 다르게 나타났다. 분리된 내생균은 참당귀 점무늬병균 Phoma sp. Y11 균주와 대치배양하여 항균활성을 측정하였다. 총 14종의내생균이균주 Y11에대해억제효과를나타내었다. 본 연구는 참당귀에서 분리한 내생균이 참당귀의 점무늬병에 대한 생물학적 방제제로 사용될 수 있다는 것을 제시하고 있다.

Keywords

Acknowledgement

This work was supported by the grants from 'Forest Science and Technology Development' (Project No. FE0702-2018-02) funded by the Korea Forest Service, Republic of Korea

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