The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Protective Effects of Individual Extract and Mixture of Forsythia Fructus and Corydalis Tuber on Chronic Pancreatitis in Mice

마우스 만성 췌장염 모델에서 연교(連翹)와 현호색(玄胡索) 단일 추출물 및 추출 혼합물의 보호 효과

  • Shin, Joon Yeon (Department of Herbology, School of Korean Medicine, Wonkwang University) ;
  • Choi, Ji-Won (Department of Herbology, School of Korean Medicine, Wonkwang University) ;
  • Kim, Dong-Uk (Hanbang Cardio-Renal Syndrome Research Center, Wonkwang University) ;
  • Zhou, Ziqi (Department of Herbology, School of Korean Medicine, Wonkwang University) ;
  • Kweon, Bitna (Department of Herbology, School of Korean Medicine, Wonkwang University) ;
  • Bae, Gi-Sang (Hanbang Cardio-Renal Syndrome Research Center, Wonkwang University) ;
  • Song, Ho-Joon (Department of Herbology, School of Korean Medicine, Wonkwang University) ;
  • Park, Sung-Joo (Department of Herbology, School of Korean Medicine, Wonkwang University)
  • 신준연 (원광대학교 한의과대학 본초학교실) ;
  • 최지원 (원광대학교 한의과대학 본초학교실) ;
  • 김동욱 (원광대학교 한방심신증후군센터) ;
  • 주자기 (원광대학교 한의과대학 본초학교실) ;
  • 권빛나 (원광대학교 한의과대학 본초학교실) ;
  • 배기상 (원광대학교 한방심신증후군센터) ;
  • 송호준 (원광대학교 한의과대학 본초학교실) ;
  • 박성주 (원광대학교 한의과대학 본초학교실)
  • Received : 2020.11.04
  • Accepted : 2021.01.25
  • Published : 2021.01.30
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Abstract

Objectives : Forsythiae Fructus has been reported to have anti-inflammatory effects in many diseases, and Corydalis Tuber has been used as a pain suppressor in Eastern Asia. However, the protective effects of individual water extract of Forsythiae Fructus (FF) and Corydalis Tuber (CT) and the mixture of FF and CT (FC) on chronic pancreatitis (CP) were not well-investigated. Therefore, we investigated the protective effects of FF, CT, and FC on CP in mice. Methods : To induce CP, cerulein was injected 6 times a day, 4 times a week for 3 weeks. 1 h before the every cerulein injection, 200 mg/kg of FF, CT, or FC was intraperitoneally injected to mice. Histological analysis of pancreas was examined by hematoxylin and eosin stain and collagen deposition was examined by Masson's trichrome stain. Fibrogenic parameters such as α-smooth muscle actin (α-SMA), extracellular matrix (ECM) deposition, and fibrotic cytokines such as transforming growth factor (TGF)-β1 and platelet-derived growth factor (PDGF) were also analyzed by immunofluorescence stain and real-time PCR. Results : Histological damages in pancreas were inhibited by pre-treatment of FF or FC but not CT. α-SMA and ECM in pancreas were inhibited by pre-treatment of CT or FC but not FF. Moreover, the expression of TGF-β1 and PDGF in pancreas were inhibited by FF, CT or FC. Conclusions : Our results suggest that FC have protective effect on CP in mice through inhibition of α-SMA, ECM, TGF-β1 and PDGF in pancreas, and these findings could suggest new clinical strategy for CP.

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References

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