The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Protective effect of Citrus unshiu peel on the cadmium-induced apoptosis in HepG2 cells

카드뮴으로 유발한 간세포 자멸사에서 진피의 보호효과

  • Noh, Gyu Pyo (College of Korean Medicine, Daegu Haany University) ;
  • Byun, Sung Hui (College of Korean Medicine, Daegu Haany University) ;
  • Lee, Jong Rok (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Park, Sook Jahr (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Kim, Sang Chan (College of Korean Medicine, Daegu Haany University)
  • 노규표 (대구한의대학교 한의과대학) ;
  • 변성희 (대구한의대학교 한의과대학) ;
  • 이종록 (대구한의대학교 제약공학과) ;
  • 박숙자 (대구한의대학교 제약공학과) ;
  • 김상찬 (대구한의대학교 한의과대학)
  • Received : 2020.10.19
  • Accepted : 2021.01.25
  • Published : 2021.01.30
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Abstract

Objective : Citrus unshiu peel (Citri Unshius Pericarpium) has been prescribed to suppress coughing and phlegm in Korean medicine. In this study, the effect of ethanol extract of Citrus unshiu peel (CEE) on apoptosis was investigated using cadmium chloride (CdCl2) treated HepG2 cells. Methods : CEE was prepared by extracting 300 g of Citri Unshius Pericarpium in 3 L of ethanol for 72 h. Apoptosis was determined by the TUNEL assay. The mitochondrial membrane potential (MMP) was monitored using the membrane-permeable fluorescent dye Rh123. The expression level of each protein was monitored by Western blot analysis. Results : CEE protected HepG2 cells from apoptosis as determined by the TUNEL assay. A decrease in MMP was observed in cells exposed to cadmium, indicating that mitochondria are involved in the induction of apoptosis. However, CEE recovered the reduction in MMP caused by cadmium. In addition, decreased expression of B-cell lymphoma 2 (Bcl-2), procaspase, and poly(ADP-ribose) polymerase (PARP) by cadmium was increased by CEE. The anti-apoptotic effect of CEE was found to be associated with inhibition of JNK and p38 phosphorylation when examining the expression of phosphorylated MAPK by Western blot. Conclusion : This study showed that CEE exerted anti-apoptotic effects in cadmium-induced HepG2 cells by inhibiting the reduction of MMP and changes in the expression level of apoptotic proteins. These results suggest the potential for CEE to be used for heavy metal-induced liver damage.

Keywords

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