Anti-inflammatory Effect of the Extract of Gynostemma pentaphyllum cell from Ullengdo Island as Korean Endemic Plant

한국 고유 식물 울릉도 돌외 식물 세포 배양추출물의 항염증 효과

  • Mok, Bo-Ram (Department of Dermatology, Bundang CHA Medical Center, CHA University School of Medicine) ;
  • Kim, Soo-Yun (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.) ;
  • Paek, Seung hye (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.) ;
  • Jang, Young-su (Department of Dermatology, Bundang CHA Medical Center, CHA University School of Medicine) ;
  • Shin, Jung U (Department of Dermatology, Bundang CHA Medical Center, CHA University School of Medicine) ;
  • Moh, Sang Hyun (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.)
  • 목보람 (CHA의과대학교 분당차병원 피부과학교실) ;
  • 김수윤 ((주)바이오에프디엔씨 안티에이징연구소) ;
  • 백승혜 ((주)바이오에프디엔씨 안티에이징연구소) ;
  • 장영수 (CHA의과대학교 분당차병원 피부과학교실) ;
  • 신정우 (CHA의과대학교 분당차병원 피부과학교실) ;
  • 모상현 ((주)바이오에프디엔씨 안티에이징연구소)
  • Received : 2020.11.03
  • Accepted : 2021.02.05
  • Published : 2021.02.28


The purpose of this study was to evaluate and verify the effectiveness of sustainable cosmetic raw materials developed from Gynostemma pentaphyllum, a plant native to Ulleungdo, in improving the skin barrier function and treating atopic dermatitis. Cells were derived from adult Gynostemma pentaphyllum plants, and suitable conditions for mass culture of the cells were established in a bioreactor. DNA components and amino acids extracted from this mass culture were identified from the HPLC fraction. In the in vitro efficacy evaluation results, changes in the expression levels of skin barrier-related proteins such as filaggrin (FLG) and Zonula occludens-1 (Zo-1) were insignificant. It was confirmed that the expression levels of the proteins thymic stromal lymphopoietin (TSLP) and interleukin-33 (IL-33) were significantly reduced. These results lead to the conclusion that Gynostemma pentaphyllum cell extracts have significant anti-inflammatory effects and that these extracts can be widely used as sustainable, nature-friendly active material in cosmetics with anti-inflammatory effects and targeted at improving atopic dermatitis.They may find use in anti-aging cosmetic products as well.

이 논문의 목적은 울릉도 자생식물인 돌외식물(GP: Gynostemma pentaphyllum)을 이용하여 지속가능한 화장품 원료 개발을 위해 피부장벽개선 및 아토피피부염 개선 효능을 평가하고 검증하는 데 있다. 자연을 훼손하지 않으며 지속가능한 항노화 소재개발을 위하여 울릉도 자생식물인 돌외에서 식물세포를 유도하여 대량배양 조건을 확립, 대량배양된 식물세포로부터 다양한 용매로 추출 후, HPLC 분석을 통하여 adenosine, guanosine 및 tyrosine, phenylalanine 변화를 확인하였다. 또한, 돌외 식물세포의 피부장벽개선효능 및 항가려움증 효능평가를 위해 Th2 사이토카인을 이용한 in vitro 염증 모델에서 피부장벽관련 인자인 FLG, Zo-1의 유전자 발현에 유의미한 변화가 확인이 되지 않았지만 항가려움증 관련 인자인 TSLP, IL-33 의 유전자 발현에 유의미한 감소 변화를 확인하였다. 따라서 돌외 식물세포 추출물이 가려움증을 개선시키는 데 유의미한 효능이 있을 것으로 확인되며, 나아가 돌외 식물세포 추출물이 지속가능한 자연친화적 활성 소재로써, 아토피피부염 개선을 위한 화장품에 널리 활용될 수 있을 것이라 사료된다.



  1. Y. Renert-Yuval, E. Guttman-Yassky, "What's New in Atopic Dermatitis", Dermatologic Clinics, Vol.37, No.2, pp.205-213, April. 2019. DOI:
  2. M. Kim, J. Kim, "Phenotypes and endotypes of atopic dermatitis: Clinical implications", Allergy Asthma & Respiratory Disease, Vol.8, No.1, pp.9-14, Jan. 2020. DOI:
  3. M. S. Kim, K. H. Kim, H. S. Yook, "Antioxidative Effects of Campanula takesimana Nakai Extract", Journal of the Korean Society of Food Science and Nutrition, Vol.41, No.10, pp.1331-37, Oct. 2012. DOI:
  4. E. H. Choi, M. Q. Man, P. Xu, S. Xin, Z. Liu, D. A. Crumrine, Y. J. Jiang, J. W. Fluhr, K. R. Feingold, P. M. Elias, T. M. Mauro, "Stratum corneum acidification is impaired in moderately aged human and murine skin", Journal of Investigative Dermatology, Vol.127, No.12, pp.2847-56, Dec. 2007. DOI:
  5. J. M. Leyva-Castillo, P. Hener, H. Jiang, M. Li, "TSLP produced by keratinocytes promotes allergen sensitization through skin and thereby triggers atopic march in mice" Journal of Investigative Dermatology, Vol.133, No.1, pp.154-63, Jul. 2013. DOI:
  6. S. K. Hwang, S. J. Lee, K. S. Oh, "Relationship between vesicularities of pumice clasts and eruption types in the Maljandeung Tuff, Ulleung Island, Korea" Journal of the Geological Society of Korea, Vol.56, No.4, pp.453-468, Aug. 2020. DOI:
  7. S. Yang, H. D. Jang, B. M. Nam, G. Y. Chung, R. Y. Lee, J. H. Lee, B. U. O, "A florisitic study of Ulleungdo Island in Korea" Korean Journal of Plant Taxonomy, Vol.45, No.2, pp.192-212, June. 2015. DOI:
  8. G. Y. Chung, M. S. Park, B. M. Nam, K. N. Hong, J. Jang, C. H. Lee, "The Regional Folk Plants in Inland of Gyeongsangbuk-do (Ι)" The Plant Resources Society of Korea. Vol.23, No.5, pp.465-479, 2010.
  9. Y. H. Choi, H. S. Jung, M. J. Cho, M. Y. Song, H. H. Seo, S. H. Moh, "Efficacy of callus induced from Ullengdo niche plants for skin protection" Journal of the Korea Academia-Industrial cooperation Society, Vol.15, No.8, pp.5070-5077, Aug. 2014. DOI:
  10. K. M. Kim, A. R. Kim, A. Y. Kim, J. H. Ha, S. H. Xuan, Y. J. Jeong, Y. M. Park, H. J. Jeong, I. G. Hong, S. N. Park, "Antioxidative and Cellular Protective Effects of Dolwoe (Gynostemma pentaphyllum Makino) Extracts against Oxidative Stress" The Korean Society of Pharmacognosy, Vol.48, No.2, pp.125-133, 2017.
  11. T. F. Tsang, B. Chan, W. C. S. Tai, G. Huang, J. Wang, X. Li, Z. H. Jiang, W. L. W. Hsiao, "Gynostemma pentaphyllum saponins induce melanogenesis and activate cAMP/PKA and Wnt/β-catenin signaling pathways" Phytomedicine, Vol.60, Jul. 2019. DOI:
  12. H. J. Kim, D. J. Lee, J. J. Ku, K. Choi, K. W. Park, S. H. Kang, C. Moon, P. J. Lee, "Anti-inflammatory Effect of Extracts from Folk Plants in Ulleung Island" Korean Journal of Plant Resources, Vol.26, No.2, pp.169-177, April. 2013. DOI:
  13. V. T. T. Huyen, D. V. Phan, P. Thang, P. T. Ky, N. K Hoa, C. G. Ostenson, "Antidiabetic Effects of Add-On Gynostemma pentaphyllum Extract Therapy with Sulfonylureas in Type 2 Diabetic Patients" Evidence-Based Complementary and Alternative Medicine, Vol 2012, Oct. 2012. DOI:
  14. P. Luthje, E. F. Lokman, C. Sandstrom, C. G. Ostenson, A. Brauner, "Gynostemma pentaphyllum exhibits anti-inflammatory properties and modulates antimicrobial peptide expression in the urinary bladder" Journal of Functional Foods, Vol 17, pp283-292, Aug. 2015. DOI:
  15. W. Y. Wong, M. M. L. Lee, B. D. Chan, V. W. S. Ma, W. Zhang, T. T. C. Yip, W. T. Wong, W. C. S. Tai, "Gynostemma pentaphyllum saponins attenuate inflammation in vitro and in vivo by inhibition of NF-κB and STAT3 signaling" Oncotarget, Vol 8, No 50, pp. 87401-87414, Sep. 2017. DOI:
  16. S. J. Brown, W. H. I. McLean, "One remarkable molecule: filaggrin" Journal of Investigative Dermatology, Vol 132, No 3, pp. 751-62, Mar. 2012. DOI:
  17. L. Eckhart, S. Lippens, E. Tschachler, W. Declercq, "Cell death by cornification" Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol 1833, No 12, pp. 3471-3480, Dec. 2013. DOI:
  18. R. Sun, A. Celli, D. Crumrine, M. Hupe, L. C. Adame, S. D. Pennypacker, K. Park, Y. Uchida, K. R. Feingold, P. M. Elias, D. Ilic, T. M. Mauro, "Lowered Humidity Produces Human Epidermal Equivalents with Enhanced Barrier Properties" Tissue Engineering Part C: Methods, Vol 21, No 1, Jun. 2014. DOI:
  19. H. Miajlovic, P. G. Fallon, A. D. Irvine, T. J. Foster, "Effect of filaggrin breakdown products on growth of and protein expression by Staphylococcus aureus" The Journal of Allergy and Clinical Immunology, Vol 126, No 6, pp. 1184-1190, Dec. 2010. DOI:
  20. H. Baurecht, A.D. Irvine, N. Novak, T. Illig, B. Buhler, J. Ring, S. Wagenpfeil, S. Weidinger, "Toward a major risk factor for atopic eczema: Meta-analysis of filaggrin polymorphism data" Journal of Allergy and Clinical Immunology, Vol 120, No 6, pp. 1406-1412, Dec. 2007. DOI:
  21. J. Esparza-Gordillo, A. Matanovic, I. Marenholz, et al., "Maternal filaggrin mutations increase the risk of atopic dermatitis in children: an effect independent of mutation inheritance" PLoS Genetics, Vol 11, No 3, Mar. 2015. DOI:
  22. C. M. Niessen, "Tight junctions/adherens junctions: basic structure and function" Journal of Investigative Dermatology, Vol 127, No 11, pp. 2525-32, Nov. 2007. DOI:
  23. D. I. S. Batista, L. Perez, R. L. Orfali, M. C. Zaniboni, L. P. Samorano, N. V. Pereira, M. N. Sotto, A. S. Ishizaki, L. M. S. Oliveira, M. N. Sato, V. Aoki, "Profile of skin barrier proteins (filaggrin, claudins 1 and 4) and Th1/Th2/Th17 cytokines in adults with atopic dermatitis" Journal of the European Academy of Dermatology and Venereology, Vol 29, No 6, pp. 1091-5, Jun. 2015. DOI:
  24. J. Klonowska, J. Glen, R. J. Nowicki, M. Trzeciak, "New Cytokines in the Pathogenesis of Atopic Dermatitis-New Therapeutic Targets" International Journal of Molecular Sciences, Vol 19, No 10, pp. 3086, Oct. 2018. DOI:
  25. S. R. Wilson, L. The, L. M. Batia, K. Beattie, G. E. Katibah, S. P. McClain, M. Pellegrino, D. M. Estandian, D. M. Bautista, "The epithelial cell-derived atopic dermatitis cytokine TSLP activates neurons to induce itch" Cell, Vol 155, No 2, pp. 285-95, Oct. 2013. DOI:
  26. I. Yasutomo, "Interleukin-33 in atopic dermatitis" Journal of Dermatological Science, Vol 96, No 1, pp. 2-7, Oct. 2019. DOI: