Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Effects of Hydrothermal Pretreatment on the Nutritional Values and In Vitro Fermentation Characteristics of Tall Fescue (Festuca arundinacea) and Corn Silage

열수 전처리에 따른 톨페스큐와 옥수수 사일리지의 영양적 가치와 in vitro 발효특성에 미치는 영향

  • Kim, Dong Hyeon (National Institute of Animal Science, Rural Development Administration) ;
  • Son, Jun Kyu (National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Ji Hwan (National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Sang Bum (Rural Development Administration) ;
  • Park, Beom Young (National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Doo San (National Institute of Animal Science, Rural Development Administration) ;
  • Jang, Gul Won (National Institute of Animal Science, Rural Development Administration) ;
  • Lim, Hyun Joo (National Institute of Animal Science, Rural Development Administration) ;
  • Hur, Tai Young (National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Eun Tae (National Institute of Animal Science, Rural Development Administration)
  • 김동현 (농촌진흥청 국립축산과학원) ;
  • 손준규 (농촌진흥청 국립축산과학원) ;
  • 이지환 (농촌진흥청 국립축산과학원) ;
  • 김상범 (농촌진흥청) ;
  • 박범영 (농촌진흥청 국립축산과학원) ;
  • 김두산 (농촌진흥청 국립축산과학원) ;
  • 장길원 (농촌진흥청 국립축산과학원) ;
  • 임현주 (농촌진흥청 국립축산과학원) ;
  • 허태영 (농촌진흥청 국립축산과학원) ;
  • 김언태 (농촌진흥청 국립축산과학원)
  • Received : 2020.08.14
  • Accepted : 2021.01.08
  • Published : 2021.01.31
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Abstract

This study examined the effects of a hydrothermal pretreatment (HP) on the nutritional values and in vitro fermentation characteristics of tall fescue and corn silage. This study was conducted through a factorial design of 2 (control or HP) × 2 (hay; tall fescue or silage; corn). For the HP, forage was placed into a glass bottle with 20% w/v of water, and the glass bottle was sealed and heated to reach a temperature of 121℃ (0.12 MPa). The solid residue and liquid were collected and oven-dried at 65℃ for three days. The dried materials were tested for in vitro fermentation at 39℃ for 24 and 48 h. The content of ADF increased significantly regardless of the forage type. After in vitro incubation for 24 h, the total VFA content was significantly lower after HP, regardless of the forage type (p ≤ 0.05), and the propionate concentration was increased in corn silage with HP (p ≤ 0.05). After 48 hours of in vitro incubation, the propionate content increased significantly (p ≤ 0.03) in corn silage with HP (p ≤ 0.05), but the butyrate content decreased significantly (p ≤ 0.05). There was no change in the in vitro dry matter and neutral detergent fiber digestibility by HP regardless of the forage type. Therefore, the use of hydrothermally pretreated corn silage could be advantageous for the supply of energy for ruminants.

본 연구는 열수 전처리에 따른 사료의 영양소 함량과 반추위내 발효특성의 변화를 알아보고자 수행되었다. 본 시험은 2(대조구 또는 열수 전처리)×2(건초; 톨페스큐 또는 사일리지; 옥수수)의 요인설계를 통해 실시하였다. 열수 전처리는 사료에 물을 20% w/v 수준하고, 멸균기를 이용하여 열처리를 20분간 실시하였다(121℃, 0.12 MPa). 제조된 시험사료는 in vitro 배양 시험을 통해 39℃에서 24시간 및 48시간동안 배양하였다. 연구결과, 열수 전처리 후 ADF의 함량은 조사료 종류에 구분없이 모두 유의적으로 증가하였다. In vitro 24시간 배양 후 total VFA는 조사료 종류에 관계 없이 열수 전처리 후에 함량이 유의적으로 낮았으며(p ≤ 0.05), Propionate 함량은 열수 전처리된 옥수수 사일리지에서 옥수수 사일리지 보다 유의적으로 증가하였다(18.9 vs. 26.6%; p ≤ 0.05). In vitro 48시간 배양 후에는 옥수수 사일리지에서 열수 천처리에 의해 propionate 함량이 유의적으로 증가하였으나(p ≤ 0.05), Butyrate 함량은 유의적으로 감소하였다(p ≤ 0.05). 건물과 NDF 소화율의 경우에는 조사료 종류에 관계없이 열수 전처리에 의한 변화가 없었다(p > 0.05). 따라서 열수 전처리된 옥수수 사일리지를 이용할 시 반추동물의 에너지원 공급에 유리할 것으로 판단된다.

Keywords

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