The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effect of Sosiho-tang on a Thioacetamide-induced Liver Fibrosis Mouse Model

소시호탕(小柴胡湯)이 thioacetamide로 유발된 간섬유증 동물 모델에 미치는 영향

  • Lee, Se Hui (Dept. of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Oh, Min Hyuck (Dept. of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Shin, Mi-Rae (Dept. of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Ji Hye (Dept. of Oriental Medicine, College of Oriental Medicine, Semyung University) ;
  • Roh, Seong-Soo (Dept. of Herbology, College of Korean Medicine, Daegu Haany University)
  • 이세희 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 오민혁 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 신미래 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이지혜 (세명대학교 한의과대학 한의예과) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2021.09.08
  • Accepted : 2021.12.20
  • Published : 2021.12.30
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Abstract

Objectives: The aim of the current study was to investigate the effect of Sosiho-tang on thioacetamide (TAA)-induced liver fibrosis in mice and to elucidate its underlying mechanisms. Methods: The mice were divided into 4 groups: Normal mice (Normal), TAA-induced control mice (Control), TAA-induced and silymarin-treated (50 mg/kg) mice (Silymarin), and TAA-induced and Sosiho-tang treated (200 mg/kg) mice (SSHT). Liver fibrosis was induced via intraperitoneal injection of TAA three times a week for 8 weeks. Silymarin and Sosiho-tang were concomitantly administered for 8 weeks. Serum and liver tissues were then collected and the anti-oxidant and inflammatory protein levels in the liver tissues were evaluated using western blotting. Results: SSHT administration significantly reduced the levels of AST, ALT, ammonia, and MPO in the serum. SSHT also significantly down-regulated liver NADPH oxidase and regulated the Nrf2/Keap1 signaling pathway. SSHT treatment downregulated the liver NF-κB levels and suppressed inflammatory cytokines. SSHT treatment also decreased bile acid-related factors, such as CYP7A1 and NTCP, and fibrosis-related factors, such as α-SMA and Collagen I. Conclusions: Taken together, these data suggest that SSHT administration suppressed the progression of liver fibrosis by activating the Nrf2/Keap1 pathway and inhibiting NF-κB.

Keywords

Acknowledgement

정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원(No. 2018R1A5A2025272)을 받아 수행된 연구입니다.

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