Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Ram semen preserved at 0℃ with soybean lecithin Tris-based extender substituted for egg yolk

  • Zhao, Jian-qing (College of Life Sciences, Tarim University) ;
  • Xiao, Guo-liang (Kashgar Animal Husbandry Workstation) ;
  • Zhu, Wen-liang (Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production & Construction Corps) ;
  • Fang, Di (College of Life Sciences, Tarim University) ;
  • Li, Na (College of Life Sciences, Tarim University) ;
  • Han, Chun-mei (Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production & Construction Corps) ;
  • Gao, Qing-hua (College of Life Sciences, Tarim University)
  • Received : 2020.02.22
  • Accepted : 2020.05.01
  • Published : 2021.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: The present study evaluated the preservation of ram semen at 0℃ using soybean lecithin with a Tris-fructose extender. Methods: Semen was collected by artificial vagina ejaculation from six rams with proven fertility. High quality ejaculates were diluted by soybean lecithin (0.25%, 0.5%, 0.75%, 1.0%, 1.25%) using Tris-fructose extender and control (Tris-fructose egg yolk extender), respectively. The ejaculates were diluted to a concentration of 5×108 sperm/mL, followed by cooling to 0℃ in 90 min and maintaining the temperature for 12 days. The diluted semen samples were examined and recorded for sperm progressive motility, acrosome integrity at 0, 24, 72, 144, 216, 288 h, respectively. Two hundred and twenty-three ewes were inseminated for 216 h with optimal soybean lecithin concentrated semen or control via trans-cervical insemination. Results: The results showed that there were no differences in sperm progressive motility at 0, 24, 72, and 144 h (p>0.05). After 216 h, the sperm progressive motility in the control group and 0.5% concentration groups was significantly higher when compared to 0.25% concentration (p<0.05). The 0.5% concentration group demonstrated the highest survival rate and had no difference with the control group (p>0.05). At 216 h, the sperm progressive motility of all groups was still above 50%. The acrosome integrity of all groups was decreased with prolongation of storage time, but there was no difference at each time point (p>0.05). There was no significant difference in the lambing rate and pregnancy rate between the 0.5% concentration group and the control group (p>0.05). Conclusion: These results suggest that ram sperm is capable of fertilization after preservation at 0℃ with 0.5% of soybean lecithin in Tris-based extender substituted for egg yolk and produce normal offspring after insemination.

Keywords

References

  1. Benson JD, Woods EJ, Walters EM, Critser JK. The cryobiology of spermatozoa. Theriogenology 2012;78:1682-99. https://doi.org/10.1016/j.theriogenology.2012.06.007
  2. Woods EJ, Benson JD, Agca Y, Critser JK. Fundamental cryobiology of reproductive cells and tissues. Cryobiology 2004;48:146-56. https://doi.org/10.1016/j.cryobiol.2004.03.002
  3. Barbas JP, Mascarenhas RD. Cryopreservation of domestic animal sperm cells. Cell Tissue Bank 2009;10:49-62. https://doi.org/10.1007/s10561-008-9081-4
  4. Papa FO, Felicio GB, Melo-Ona CM, et al. Replacing egg yolk with soybean lecithin in the cryopreservation of stallion semen. Anim Reprod Sci 2011;129:73-7. https://doi.org/10.1016/j.anireprosci.2011.10.006
  5. Akhter S, Ansari MS, Andrabi SMH, Ullah N, Qayyum M. Effect of antibiotics in extender on bacterial and spermatozoal quality of cooled buffalo (Bubalus bubalis) bull semen. Reprod Domest Anim 2008;43:272-8. https://doi.org/10.1111/j.1439-0531.2007.00890.x
  6. Althouse GC. Sanitary procedures for the production of extended semen. Reprod Domest Anim 2008;43:374-8. https://doi.org/10.1111/j.1439-0531.2008.01187.x
  7. Holt WV. Fundamental aspects of sperm cryobiology: the importance of species and individual differences. Theriogenology 2000;53:47-58. https://doi.org/10.1016/S0093-691X(99)00239-3
  8. Chelucci S, Pasciu V, Succu S, et al. Soybean lecithin-based extender preserves spermatozoa membrane integrity and fertilizing potential during goat semen cryopreservation. Theriogenology 2015;83:1064-74. https://doi.org/10.1016/j.theriogenology.2014.12.012
  9. Andrabi SMH. Factors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa. Reprod Domest Anim 2009;44:552-69. https://doi.org/10.1111/j.1439-0531.2008.01240.x
  10. Akhter S, Ansari MS, Andrabi SMH, Rakha BA, Ullah N, Khalid M. Soya-lecithin in extender improves the freezability and fertility of buffalo (Bubalus bubalis) bull spermatozoa. Reprod Domest Anim 2012;47:815-9. https://doi.org/10.1111/j.1439-0531.2011.01973.x
  11. Aires VA, Hinsch KD, Mueller-Schloesser F, Bonger K, Mueller-Schloesser S, Hinsch E. In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen. Theriogenology 2003;60:269-79. https://doi.org/10.1016/S0093-691X(02)01369-9
  12. Forouzanfar M, Sharafi M, Hosseini SM, et al. In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen. Theriogenology 2010;73:480-7. https://doi.org/10.1016/j.theriogenology.2009.10.005
  13. Roof DJ, Bowley S, Price LL, Matsas DJ. Comparison of two commercial extenders for cryopreservation of goat semen without sperm washing. Theriogenology 2012;77:412-20. https://doi.org/10.1016/j.theriogenology.2011.08.015
  14. Salmani H, Towhidi A, Zhandi M, Bahreini M, Sharafi M. In vitro assessment of soybean lecithin and egg yolk based diluents for cryopreservation of goat semen. Cryobiology 2014;68: 276-80. https://doi.org/10.1016/j.cryobiol.2014.02.008
  15. Zhao J, Wang J, Li N, Gao Q. Effect of containing soybean lecithin instead of yolk dilution for low temperature preservation of sheep semen. J Tarim Univ 2018;30:15-9.
  16. Quinn PJ, Salamon S, White IG. The effect of cold shock and deep-freezing on ram spermatozoa collected by electrical ejaculation and by an artificial vagina. Aust J Agric Res 1968;19:119-28. https://doi.org/10.1071/AR9680119
  17. de Paz P, Esteso MC, Alvarez M, Mata M, Chamorro CA, Anel L. Development of extender based on soybean lecithin for its application in liquid ram semen. Theriogenology 2010;74:663-71. https://doi.org/10.1016/j.theriogenology.2010.03.022
  18. Toker MB, Alcay S, Gokce E, Ustuner B. Cryopreservation of ram semen with antioxidant supplemented soybean lecithin-based extenders and impacts on incubation resilience. Cryobiology 2016;72:205-9. https://doi.org/10.1016/j.cryobiol.2016.05.001
  19. Masoudi R, Sharafi M, Shahneh AZ, et al. Fertility and flow cytometry study of frozen-thawed sperm in cryopreservation medium supplemented with soybean lecithin. Cryobiology 2016;73:69-72. https://doi.org/10.1016/j.cryobiol.2016.05.010
  20. Kmenta I, Strohmayer C, Muller-Schlosser F, Schafer-Somi S. Effects of a lecithin and catalase containing semen extender and a second dilution with different enhancing buffers on the quality of cold-stored canine spermatozoa. Theriogenology 2011;75:1095-103. https://doi.org/10.1016/j.theriogenology.2010.11.018
  21. Reed ML, Ezeh PC, Hamic A, Thompson DJ, Caperton CL. Soy lecithin replaces egg yolk for cryopreservation of human sperm without adversely affecting postthaw motility, morphology, sperm DNA integrity, or sperm binding to hyaluronate. Fertil Steril 2009;92:1787-90. https://doi.org/10.1016/j.fertnstert.2009.05.026
  22. Vick MM, Bateman HL, Swanson WF. 97 Improved cryopreservation of domestic cat spermatozoa in a soy lecithin-based extender. Reprod Fertil Dev 2010;23:153-4. https://doi.org/10.1071/RDv23n1Ab97
  23. Roof DJ, Bowley S, Price LL, Matsas DJ. Comparison of two commercial extenders for cryopreservation of goat semen without sperm washing. Theriogenology 2012;77:412-20. https://doi.org/10.1016/j.theriogenology.2011.08.015
  24. Bencharif D, Amirat L, Anton M, et al. The advantages of LDL (low density lipoproteins) in the cryopreservation of canine semen. Theriogenology 2008;70:1478-88. https://doi.org/10.1016/j.theriogenology.2008.06.095
  25. Najafi A, Kia HD, Mohammadi H, et al. Different concentrations of cysteamine and ergothioneine improve microscopic and oxidative parameters in ram semen frozen with a soybean lecithin extender. Cryobiology 2014;69:68-73. https://doi.org/10.1016/j.cryobiol.2014.05.004
  26. Mehdipour M, Kia HD, Najafi A, Dodaran HV, Garcia-Alvarez O. Effect of green tea (Camellia sinensis) extract and prefreezing equilibration time on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology 2016;73:297-303. https://doi.org/10.1016/j.cryobiol.2016.10.008
  27. Salamon S, Maxwell WMC. Storage of ram semen. Anim Reprod Sci 2000;62:77-111. https://doi.org/10.1016/S0378-4320(00)00155-X
  28. Salamon S, Maxwell WMC. Frozen storage of ram semen II. Causes of low fertility after cervical insemination and methods of improvement. Anim Reprod Sci 1995;38:1-36. https://doi.org/10.1016/0378-4320(94)01328-J
  29. van Wagtendonk-de Leeuw AM, Haring RM, Kaal-Lansbergen LMTE, den Daas JHG. Fertility results using bovine semen cryopreserved with extenders based on egg yolk and soy bean extract. Theriogenology 2000;54:57-67. https://doi.org/10.1016/S0093-691X(00)00324-1
  30. Salmani H, Towhidi A, Zhandi M, Bahreini M, Sharafi M. In vitro assessment of soybean lecithin and egg yolk based diluents for cryopreservation of goat semen. Cryobiology 2014;68:276-80. https://doi.org/10.1016/j.cryobiol.2014.02.008

Cited by

  1. Effects of Three Semen Extenders, Breeding Season Month and Freezing-Thawing Cycle on Spermatozoa Preservation of Portuguese Merino Sheep vol.11, pp.9, 2021, https://doi.org/10.3390/ani11092619