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Effects of wilting and additives on the ensiling quality and in vitro rumen fermentation characteristics of sudangrass silage

  • Wan, Jiang Chun (College of Animal Science and Technology, China Agricultural University) ;
  • Xie, Kai Yun (College of Grassland and Environment Science, Xinjiang Agricultural University) ;
  • Wang, Yu Xiang (College of Grassland and Environment Science, Xinjiang Agricultural University) ;
  • Liu, Li (College of Grassland and Environment Science, Xinjiang Agricultural University) ;
  • Yu, Zhu (College of Animal Science and Technology, China Agricultural University) ;
  • Wang, Bing (College of Animal Science and Technology, China Agricultural University)
  • Received : 2020.02.11
  • Accepted : 2020.05.13
  • Published : 2021.01.01

Abstract

Objective: This study was conducted to investigate the effects of molasses and Lactobacillus plantarum on the ensiling quality and in vitro rumen fermentation of sudangrass silage prepared with or without wilting. Methods: The ensiling experiment, measured with 3 replicates, was carried out according to a 2×4 (wilted stages×additives) factorial treatment structure. Dry matter of the fresh (210 g/kg fresh matter) or wilted (305 g/kg fresh matter) sudangrass were ensiled (packed into 5.0-L plastic jars) without additive (control) or with molasses (M), Lactobacillus plantarum (LP), or molasses + Lactobacillus plantarum (M+LP). After 60 days of ensiling, the silages were analyzed for the chemical, fermentation, and in vitro characteristics. Results: After 60 days of ensiling, the fermentation parameters were affected by wilted, the additives and the interactions of wilted with the additives (p<0.05). The M+LP treatment at wilted had higher lactic acid levels and V-score (p<0.05) but lower pH values and butyric acid concentrations than the other treatments. In comparison with sudangrass before ensiling, after ensiling had lower dry matter and higher non-fibrous carbohydrate. The in vitro gas production, in vitro dry matter digestibility, in vitro crude protein digestibility, and in vitro acid fiber detergent digestibility changed under the effects of the additives. Significant interactions were observed between wilted and the additives in terms of in vitro gas production at 48 h, asymptotic gas production, gas production rate, half time, and the average gas production rate. The total volatile fatty acid levels in the additive treatments were higher than those in the control. Conclusion: Wilting and supplementation with molasses and Lactobacillus plantarum had the ability to improve the ensiling quality and in vitro nutrient digestibility of sudangrass silage. The M+LP treatment at wilted exhibited the strongest positive effects on silage quality and in vitro ruminal fermentation characteristics.

Keywords

Acknowledgement

This work was financially supported by the National Forage Industry System (CARS-34), Grassology Peak Discipline Foundation of Xinjiang Uygur Autonomous Region, China; the Key Research and Development Program in Ningxia Hui Autonomous Region (2017BY082), the Agricultural Technology Test demonstration and Service Support Program in China (131821301064071005), the Natural Science Foundation of Xinjiang of China (2017D01B19), and the Scientific Research Program of the Higher Education Institution of Xinjiang of China (XJEDU2016S049); National Key R&D Program of China (2017YFE0109200).

References

  1. Kondo M, Kita K, Yokota H. Effects of tea leaf waste of green tea, oolong tea, and black tea addition on sudangrass silage quality and in vitro gas production. J Sci Food Agric 2004;84:721-7. https://doi.org/10.1002/jsfa.1718
  2. Guo G, Yu CQ, Wang Q, Xin PC, Shimojo M, Shao T. Silage fermentation characteristics of Italian ryegrass (Lolium multiflorum Lam.) harvested at various times on a sunny day. Crop Sci 2014;54:851-8. https://doi.org/10.2135/cropsci2012.12.0692
  3. Zhang XQ, Jin YM, Zhang YJ, Yu Z, Yan WH. Silage quality and preservation of Urtica cannabina ensiled alone and with additive treatment. Grass Forage Sci 2014;69:405-14. https://doi.org/10.1111/gfs.12036
  4. Kang S, Wanapat M, Nunoi A. Effect of urea and molasses supplementation on quality of cassava top silage. J Anim Feed Sci 2018;27:74-80. http://doi.org/10.22358/jafs/85544/2018
  5. Contreras-Govea FE, Muck RE, Broderick GA , Weimer PJ. Lactobacillus plantarum effects on silage fermentation and in vitro microbial yield. Anim Feed Sci Technol 2013;179:61-8. https://doi.org/10.1016/j.anifeedsci.2012.11.008
  6. Ellis JL, Bannink A, Hindrichsen IK, et al. The effect of lactic acid bacteria included as a probiotic or silage inoculant on in vitro rumen digestibility, total gas and methane production. Anim Feed Sci Technol 2016;211:61-74. https://doi.org/10.1016/j.anifeedsci.2015.10.016
  7. McDonald P. The biochemistry of silage. Chichester, UK: John Wiley & Sons, Ltd.; 1981.
  8. Owens VN, Albrecht KA, Muck RE. Protein degradation and ensiling characteristics of red clover and alfalfa wilted under varying levels of shade. Can J Plant Sci 1999;79:209-22. https://doi.org/10.4141/P98-034
  9. Mertens DR. Gravimetric determination of amylase-treated neutral detergent fiber in feeds with refluxing in beakers or crucibles: collaborative study. J AOAC Int 2002;85:1217-40.
  10. Baur FJ, Ensminger LG. The Association of Official Analytical Chemists (AOAC). J Am Oil Chem Soc 1977;54:171-2. https://doi.org/10.1007/BF02670789
  11. Playne MJ, McDonald P. The buffering constituents of herbage and of silage. J Sci Food Agric 1966;17:264-8. https://doi.org/10.1002/jsfa.2740170609
  12. Murphy RP. A method for the extraction of plant samples and the determination of total soluble carbohydrates. J Sci Food Agric 1958;9:714-7. https://doi.org/10.1002/jsfa.2740091104
  13. Broderick GA, Kang JH. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media 1. J Dairy Sci 1980;63:64-75. https://doi.org/10.3168/jds.S0022-0302(80)82888-8
  14. Takahashi T, Horiguchi K, Goto M. Effect of crushing unhulled rice and the addition of fermented juice of epiphytic lactic acid bacteria on the fermentation quality of whole crop rice silage, and its digestibility and rumen fermentation status in sheep. Anim Sci J 2005;76:353-8. https://doi.org/10.1111/j.1740-0929.2005.00275.x
  15. France J, Dijkstra J, Dhanoa MS, Lopez S, Bannink A. Estimating the extent of degradation of ruminant feeds from a description of their gas production profiles observed in vitro: derivation of models and other mathematical considerations. Br J Nutr 2000;83:143-50. https://doi.org/10.1017/S0007114500000180
  16. Carro MD, Ranilla MJ, Tejido ML. Using an in vitro gas production technique to examine feed additives: effects of correcting values for different blanks. Anim Feed Sci Technol 2005;123:173-84. https://doi.org/10.1016/j.anifeedsci.2005.04.045
  17. Orskov ER. Manipulation of rumen fermentation for maximum food utilization. In: Bourne GH, editor. World review of nutrition and dietetics. Basel, Switzerland: Karger; 1975. pp. 152-82. https://doi.org/10.1159/000397977
  18. Nkosi BD, Meeske R, Van der Merwe HJ, Groenewald IB. Effects of homofermentative and heterofermentative bacterial silage inoculants on potato hash silage fermentation and digestibility in rams. Anim Feed Sci Technol 2010;157:195-200. https://doi.org/10.1016/j.anifeedsci.2010.03.008
  19. Denek N, Can A, Avci M, Aksu T, Durmaz H. The effect of molasses-based pre-fermented juice on the fermentation quality of first-cut lucerne silage. Grass Forage Sci 2011;66:243-50. https://doi.org/10.1111/j.1365-2494.2011.00783.x
  20. Kung L, Shaver RD, Grant RJ, Schmidt RJ. Silage review: interpretation of chemical, microbial, and organoleptic components of silages. J Dairy Sci 2018;101:4020-33. https://doi.org/10.3168/jds.2017-13909
  21. Li P, Tang H, Feng T, et al. Effects of UV radiation on the process of wilting and application of additives at ensiling on the fermentation quality of Siberian wildrye silage on the Qinghai-Tibetan plateau. Grassl Sci 2018;64:61-8. https://doi.org/10.1111/grs.12174
  22. McDonald P, Edwards RA, Greenhalgh JFD, Morgan CA, Sinclair LA. Animal nutrition. Harlow, Essex, UK: Longman Scientific & Technical; 1995.
  23. Zhang YC, Wang XK, Li DX, Lin YL, Yang FY, Ni KK. Impact of wilting and additives on fermentation quality and carbohydrate composition of mulberry silage. Asian-Australas J Anim Sci 2020;33:254-63. https://doi.org/10.5713/ajas.18.0925
  24. Adesogan AT, Salawu MB. The effect of different additives on the fermentation quality, aerobic stability and in vitro digestibility of pea/wheat bi-crop silages containing contrasting pea to wheat ratios. Grass Forage Sci 2002;57:25-32. https://doi.org/10.1046/j.1365-2494.2002.00298.x
  25. Muck RE, Nadeau EMG, McAllister TA, Contreras-Govea FE, Santos MC, Kung L. Silage review: recent advances and future uses of silage additives. J Dairy Sci 2018;101:3980-4000. https://doi.org/10.3168/jds.2017-13839
  26. Jahanzad E, Sadeghpour A, Hashemi M, Keshavarz Afshar R, Hosseini MB, Barker AV. Silage fermentation profile, chemical composition and economic evaluation of millet and soya bean grown in monocultures and as intercrops. Grass Forage Sci 2016;71:584-94. https://doi.org/10.1111/gfs.12216
  27. Palmer MJA, Jessop NS, Fawcett R, Illius AW. Interference of indirect gas produced by grass silage fermentation acids in an in vitro gas production technique. Anim Feed Sci Technol 2005;123:185-96. https://doi.org/10.1016/j.anifeedsci.2005.04.049
  28. Silva LS, Bezerra LR, Silva AMA, Carneiro H, Moreira MN, Oliveira RL. In vitro degradation and gas production of glycerin generated in the biodiesel production chain. Acta Sci 2015;37:265-72. https://doi.org/10.4025/actascianimsci.v37i3.27308
  29. Muck RE, Filya I, Contreras-Govea FE. Inoculant effects on alfalfa silage: in vitro gas and volatile fatty acid production. J Dairy Sci 2007;90:5115-25. https://doi.org/10.3168/jds.2006-878
  30. Chen L, Guo G, Yuan XJ, Zhang J, Li JF, Shao T. Effects of applying molasses, lactic acid bacteria and propionic acid on fermentation quality, aerobic stability and in vitro gas production of total mixed ration silage prepared with oatcommon vetch intercrop on the Tibetan Plateau. J Sci Food Agric 2016;96:1678-85. https://doi.org/10.1002/jsfa.7271

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