Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Assessment of pregnancy-associated glycoprotein profile in milk for early pregnancy diagnosis in goats

  • Singh, Shiva Pratap (Animal Physiology and Reproduction Division, Indian Council of Agricultural Research-Central Institute for Research on Goats) ;
  • Natesan, Ramachandran (Animal Physiology and Reproduction Division, Indian Council of Agricultural Research-Central Institute for Research on Goats) ;
  • Sharma, Nandini (Animal Physiology and Reproduction Division, Indian Council of Agricultural Research-Central Institute for Research on Goats) ;
  • Goel, Anil Kumar (Animal Physiology and Reproduction Division, Indian Council of Agricultural Research-Central Institute for Research on Goats) ;
  • Singh, Manoj Kumar (Animal Genetics and Breeding Division, Indian Council of Agricultural Research-Central Institute for Research on Goats) ;
  • Kharche, Suresh Dinkar (Animal Physiology and Reproduction Division, Indian Council of Agricultural Research-Central Institute for Research on Goats)
  • Received : 2019.05.16
  • Accepted : 2019.12.24
  • Published : 2021.01.01
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Abstract

Objective: This study was conducted to assess the level of pregnancy-associated glycoprotein (PAG) in whole and skim milk samples, and its suitability for early pregnancy diagnosis in goats. Methods: A two-step sandwich enzyme-linked immunosorbent assay (ELISA) system for estimation of milk PAG was developed and validated, which employed caprine-PAG specific polyclonal antisera. Whole and skim milk samples (n = 210 each) from fifteen multiparous goats were collected on alternate days from d 10 to d 30, and thereafter weekly till d 51 post-mating. PAG levels in milk samples were estimated by ELISA and the pregnancies were confirmed at d40 post-mating by transrectal ultrasonography (TRUS). Results: The level of PAG in whole and skim milk samples of both pregnant and nonpregnant goats remained below the threshold values until d 24 after mating. Thereafter, PAG concentration in whole and skim milk increased steadily in pregnant goats, whereas it continued below the threshold in non-pregnant does. The PAG profiles in whole and skim milk of pregnant goats were almost similar and exhibited strong positive relationship (r = 0.891; p<0.001). Day 26 post-mating was identified as the first time-point for significantly (p<0.05) higher milk PAG concentration in pregnant goats than to non-pregnant goats. When compared to TRUS examination for pregnancy diagnosis, the accuracy and specificity of PAG ELISA using whole and skim milk samples were 94.5% and 95.4%; and 95.3% and 100%, respectively. The high values of area-under-curve (0.904 [whole milk] and 0.922 [skim milk]), demonstrate outstanding discrimination ability of the milk assays. Among the sampling dates chosen, d 37 post-mating was identified as the best suitable time point for collection of milk samples to detect pregnancy in goats. Conclusion: The PAG concentration in whole and skim milk of goats collected between days 26 and 51 post-breeding can be used for the accurate prediction of pregnancy and may be useful for assisting management decisions in goat flocks.

Keywords

References

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