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Testicular and epididymal ultrasonography for the assessment of semen quality in the indigenous ram

  • Sarker, Suchana (Central Artificial Insemination laboratory) ;
  • Zohara, Begum Fatema (Department of Medicine, Surgery and Obstetrics, Hajee Mohammad Danesh Science and Technology University) ;
  • Azizunnesa, Azizunnesa (Department of Medicine and Surgery, Chittagong Veterinary and Animal Science University) ;
  • Islam, Md. Faruk (Department of Medicine, Surgery and Obstetrics, Hajee Mohammad Danesh Science and Technology University) ;
  • Bari, Farida Yeasmin (Department of Surgery and Obstetrics, Bangladesh Agricultural University)
  • 투고 : 2021.11.17
  • 심사 : 2021.11.26
  • 발행 : 2021.12.31

초록

The aims of this study were to measure the ultrasonographic biometry of genitalia of the indigenous rams and observe the relationship of biometry on semen parameters. The epididymal volume was significantly reduced (p < 0.01) after semen collection compared with before collection for both left and right part in all rams. The cumulative results showed that although there was no significant difference in length, width and volume of epididymis between before and after semen collection, however the values were lower after collection. The epididymal length was significantly correlated with epididymal volume (p < 0.01), semen motility (p < 0.05) and semen morphology (p < 0.01). Epididymal width was only significantly correlated with epididymal volume (p < 0.01) not with the semen parameters. Epididymal volume had a significant correlation only with semen morphology (p < 0.01).The scrotal circumference had the significant correlation with semen density, mass activity, concentration and motility (p < 0.01). The epididymis had the similar or slightly increased echogenicity as compared to the normal testis. During whole study, some white spots were found on testis which did not affect the semen quantity and quality. Significant variation was observed only for semen concentration and motility among the rams (p < 0.05). The overall normal morphology was 90.5 ± 4.6% with highest percentage of coiled tail abnormalities.

키워드

과제정보

The authors are grateful to Bangladesh Academy of Science (BAS-USDA) and Department of Surgery and Obstetrics, Bangladesh Agricultural University, Mymensingh-2202 for financial and logistic support of this study.

참고문헌

  1. Ahmad N, Noakes DE, Subandrio AL. 1991. B-mode real time ultrasonographic imaging of the testis and epididymis of sheep and goats. Vet. Rec. 128:491-496. https://doi.org/10.1136/vr.128.21.491
  2. Cartee RE, Gray BW, Powe TA, Hudson RS, Whitesides J. 1989. Preliminary implications of B-mode ultrasonography of the testicles of beef bulls with normal breeding soundness examinations. Theriogenology 31:1149-1157. https://doi.org/10.1016/0093-691X(89)90083-6
  3. Gergatz E. 2007. [Artificial insemination of sheep]. In: Pecsi T (Ed.), [Artificial Insemination of Domestic Mammals], Mezogazda Press, Budapest, pp. 335-367. Hungarian.
  4. Gouletsou PG, Amiridis GS, Cripps PJ, Lainas T, Deligiannis K, Saratsis P, Fthenakis GC. 2003. Ultrasonographic appearance of clinically healthy testicles and epididymides of rams. Theriogenology 59:1959-1972. https://doi.org/10.1016/S0093-691X(02)01259-1
  5. Guerrero VH, Huanca LW, Raymundo TF, Huerta OS, Ramos D. 2009. [Hypertonic extenders in the cryopreservation of ovine semen]. Rev. Investig. Vet. Peru 20:41-46. Spanish.
  6. Herman HA and Madden FW. 1963. The Artificial Insemination of Dairy and Beef Cattle: A Handbook and Laboratory Manual. Lucas Brothers, Columbia, pp. 579-610.
  7. Ibrahim AA, Aliyu J, Ashiru M, Jamilu M. 2012. Biometric study of the reproductive organs of three breeds of sheep in Nigeria. Int. J. Morphol. 30:1597-1603. https://doi.org/10.4067/S0717-95022012000400053
  8. Jha PK, Alam MGS, Al Mansur MA, Talukder MRI, Naher N, Rahman AKMA, Hall DC, Bari FY. 2020. Effects of number of frozen-thawed ram sperm and number of inseminations on fertility in synchronized ewes under field condition. J. Anim. Reprod. Biotechnol. 35:190-197. https://doi.org/10.12750/JARB.35.2.190
  9. Kastelic JP, Cook RB, Pierson RA, Coulter GH. 2001. Relationships among scrotal and testicular characteristics, sperm production, and seminal quality in 129 beef bulls. Can. J. Vet. Res. 65:111-115.
  10. Kulaksiz R, Cebi C, Akcay E. 2012. The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 ℃. Turk. J. Vet. Anim. Sci. 36:177-182.
  11. Kahn W. 2004. Veterinary Reproductive Ultrasonography: Horse, Cattle, Sheep, Goat, Pig, Dog, Cat. Schlutersche, Hannover, pp. 256.
  12. Liu X, Hart EJ, Petrik JJ, Nykamp SG, Bartlewski PM. 2008. Relationships between ultrasonographic image attributes, histomorphology and proliferating cell nuclear antigen expression of bovine antral follicles and corpora lutea ex situ. Reprod. Domest. Anim. 43:27-34. https://doi.org/10.1111/j.1439-0531.2008.01233.x
  13. Palasz AT, Cates WF, Barth AD, Mapletoft RJ. 1994. The relationship between scrotal circumference and quantitative testicular traits in yearling beef bulls. Theriogenology 42:715-726. https://doi.org/10.1016/0093-691X(94)90388-Y
  14. Pervage S, Hassan MR, Ershaduzzaman M, Khandoker MAMY. 2009. Preservation of liquid semen and artificial insemination in native sheep. J. Bangladesh Agric. Univ. 7:305-308. https://doi.org/10.3329/jbau.v7i2.4739
  15. Pugh CR, Konde LJ, Park RD. 1990. Testicular ultrasound in the normal dog. Vet. Radiol. 31:195-199. https://doi.org/10.1111/j.1740-8261.1990.tb01810.x
  16. Soderquist L. 1991. Sperm characteristics and fertility in dairy AI bull. Phd thesis, Swedish University of Agricultural Sciences.
  17. Tatsunami S, Matsumiya K, Tsujimura A, Itoh N, Sasao T, Koh E, Maeda Y, Eguchi J, Takehara K, Nishida T, Miyano S, Tabata C, Iwamoto T. 2006. Inter/intra investigator variation in orchidometric measurements of testicular volume by ten investigators from five institutions. Asian J. Androl. 8:373-378. https://doi.org/10.1111/j.1745-7262.2006.00143.x
  18. Zaher HA, Alawaash SA, Swelum AA. 2020. Effects of season and breed on the reproductive performance of sheep. J. Anim. Reprod. Biotechnol. 35:149-154. https://doi.org/10.12750/JARB.35.2.149