Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Green Synthesis of Copper Nano-Drug and Its Dental Application upon Periodontal Disease-Causing Microorganisms

  • El-Rab, Sanaa M.F. Gad (Department of Biotechnology, Faculty of Science, Taif University) ;
  • Basha, Sakeenabi (Department of Preventive and Community Dentistry, Faculty of Dentistry, Taif University) ;
  • Ashour, Amal A. (Department of Oral and Maxillofacial Surgery and Diagnostic Sciences, Oral Pathology Division, Faculty of Dentistry, Taif University) ;
  • Enan, Enas Tawfik (Dental Biomaterials, Faculty of Dentistry, Taif University) ;
  • Alyamani, Amal Ahmed (Department of Biotechnology, Faculty of Science, Taif University) ;
  • Felemban, Nayef H. (Preventive dentistry department, Faculty of Dentistry, Taif University)
  • Received : 2021.06.04
  • Accepted : 2021.09.06
  • Published : 2021.12.28
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Abstract

Dental pathogens lead to chronic diseases like periodontitis, which causes loss of teeth. Here, we examined the plausible antibacterial efficacy of copper nanoparticles (CuNPs) synthesized using Cupressus macrocarpa extract (CME) against periodontitis-causing bacteria. The antimicrobial properties of CME-CuNPs were then assessed against oral microbes (M. luteus. B. subtilis, P. aerioginosa) that cause periodontal disease and were identified using morphological/ biochemical analysis, and 16S-rRNA techniques. The CME-CuNPs were characterized, and accordingly, the peak found at 577 nm using UV-Vis spectrometer showed the formation of stable CME-CuNPs. Also, the results revealed the formation of spherical and oblong monodispersed CME-CuNPs with sizes ranged from 11.3 to 22.4 nm. The FTIR analysis suggested that the CME contains reducing agents that consequently had a role in Cu reduction and CME-CuNP formation. Furthermore, the CME-CuNPs exhibited potent antimicrobial efficacy against different isolates which was superior to the reported values in literature. The antibacterial efficacy of CME-CuNPs on oral bacteria was compared to the synergistic solution of clindamycin with CME-CuNPs. The solution exhibited a superior capacity to prevent bacterial growth. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and fractional inhibitory concentration (FIC) of CME-CuNPs with clindamycin recorded against the selected periodontal disease-causing microorganisms were observed between the range of 2.6-3.6 ㎍/ml, 4-5 ㎍/ml and 0.312-0.5, respectively. Finally, the synergistic antimicrobial efficacy exhibited by CME-CuNPs with clindamycin against the tested strains could be useful for the future development of more effective treatments to control dental diseases.

Keywords

Acknowledgement

We would like to express our gratitude to Deanship of Scientific Research, Taif University, Taif, Saudi Arabia for financial support under the research project number (1/439/6084). This study was approved by the Research Ethics Committee of Taif University, Taif, Saudi Arabia (No. 41- 1107-00152). The authors declare that the funding bodies had no role in the design of the study, the collection, analysis, and interpretation of data, or in writing the manuscript.

References

  1. Global, regional, and national incidence, prevalence, and years lived with disability for 354 diseases and injuries for 195 countries and territories, 1990-2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet 392: 1789-8583. https://doi.org/10.1016/s0140-6736(18)32279-7
  2. Dias HB, Carrera ET, Bortolatto JF, De Andrade MF, De Souza Rastelli AN. 2016. LED and low-level laser therapy association in tooth bleaching using a novel low concentration H2O2/N-doped TiO2 bleaching agent. Laser Phys. 26: 015602. https://doi.org/10.1088/1054-660X/26/1/015602
  3. Petersen PE, Ogawa H. 2012. The global burden of periodontal disease: Towards integration with chronic disease prevention and control. Periodontol. 2000. 60: 15-39. https://doi.org/10.1111/j.1600-0757.2011.00425.x
  4. Chen X, Wu G, Feng Z, Dong Y, Zhou W, Li B, et al. 2016. Advanced biomaterials and their potential applications in the treatment of periodontal disease. Crit. Rev. Biotechnol. 36: 760-775. https://doi.org/10.3109/07388551.2015.1035693
  5. Osorio R, Alfonso-Rodriguez CA, Medina-Castillo AL, Alaminos M, Toledano M. 2016. Bioactive polymeric nanoparticles for periodontal therapy. PLoS One 11: e0166217. https://doi.org/10.1371/journal.pone.0166217
  6. Knight ET, Liu J, Seymour GJ, Faggion Jr. CM, Cullinan MP. 2016. Risk factors that may modify the innate and adaptive immune responses in periodontal diseases. Periodontol. 2000. 71: 22-51. https://doi.org/10.1111/prd.12110
  7. Souto R, Silva-Boghossian CM, Colombo APV. 2014. Prevalence of Pseudomonas aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with chronic periodontal infection. Braz. J. Microbiol. 45: 495-501. https://doi.org/10.1590/S1517-83822014000200017
  8. Viciani E, Montagnani F, Tordini G, Romano A, Salerni L, De Luca A, et al. 2017. Prevalence of M75 Streptococcus pyogenes strains harboring slaA gene in patients affected by pediatric obstructive sleep apnea syndrome in central Italy. Front. Microbiol. 8: 294. https://doi.org/10.3389/fmicb.2017.00294
  9. Culotti A, Packman AI. 2014. Pseudomonas aeruginosa promotes Escherichia coli biofilm formation in nutrient-limited medium. PLoS One 9: e107186. https://doi.org/10.1371/journal.pone.0107186
  10. Colombo AP, Magalhaes CB, Hartenbach FA, do Souto RM, da Silva-Boghossian CM. 2016. Periodontal-disease-associated biofilm: a reservoir for pathogens of medical importance. Microb. Pathog. 94:27-34. https://doi.org/10.1016/j.micpath.2015.09.009
  11. Sudiono J, Sandra F, Halim NS, Kadrianto TA, Melinia M. 2013. Bactericidal and cytotoxic effects of Erythrina fusca leaves aquadest extract. Dent. J. Majal. Kedokt. Gigi 46: 9-13. https://doi.org/10.20473/j.djmkg.v46.i1.p9-13
  12. Mah, TF. 2012. Biofilm-specific antibiotic resistance. Future Microbiol. 7: 1061-1072. https://doi.org/10.2217/fmb.12.76
  13. Victor T Noronha , Amauri J Paula, Gabriela Duran, Andre Galembeck , Karina Cogo-Muller, Michelle Franz-Montan. et al. 2017. Silver nanoparticles in dentistry. Dent. Mater. 33: 1110-1126. https://doi.org/10.1016/j.dental.2017.07.002
  14. Roshna T, Nandakumar K. 2012. Generalized aggressive periodontitis and its treatment options: case reports and review of the literature. Case Rep. Med. 2012: 535321. https://doi.org/10.1155/2012/535321
  15. Michaud DS, Fu Z, Shi J, Chung M. 2017. Periodontal disease, tooth loss, and cancer risk. Epidemiol. Rev. 39: 49-58. https://doi.org/10.1093/epirev/mxx006
  16. Rieuwpassa IE, Achmad H, Rahmasari R. 2019. Effectiveness of clindamycin in treatment of Periodontitis. Indian J. Public Health Res. Dev. 10:1223. https://doi.org/10.5958/0976-5506.2019.03687.8
  17. Wyszogrodzka G, Marszalek B, Gil B, Dorozynski P. 2016. Metalorganic frameworks: mechanisms of antibacterial action and potential applications. Drug Discov. Today 21: 1009-1018. https://doi.org/10.1016/j.drudis.2016.04.009
  18. Doaz-Visurraga J, Daza C, Pozo C, Becerra A, von Plessing C, Garcoa 2012. A study on antibacterial alginate-stabilized copper nanoparticles by FT-IR and 2D-IR correlation spectroscopy. Int. J. Nanomed. 7:3597.
  19. Holla G, Yeluri R, Munshi AK. 2012. Evaluation of minimum inhibitory and minimum bactericidal concentration of nano-silver base inorganic anti-microbial agent (Novaron) against Streptococcus mutans. Contemp. Clin. Dent. 3: 288-293. https://doi.org/10.4103/0976-237X.103620
  20. Greenstein G, Tonetti M. 2000. The role of controlled drug delivery for periodontitis. The Research, Science and Therapy Committee of the American Academy of Periodontology. J. Periodontol. 71: 125-140. https://doi.org/10.1902/jop.2000.71.1.125
  21. Rajeshkumar S. 2016. Anticancer activity of eco-friendly gold nanoparticles against lung and liver cancer cells. J. Genet. Eng. Biotechnol. 14:195-202. https://doi.org/10.1016/j.jgeb.2016.05.007
  22. Podstawczyk D, Pawlowska A, Bastrzyk A, Czeryba M, Oszmia'nski J. 2019. Reactivity of (+)-catechin with copper (II) ions: the green synthesis of size-controlled Sub-10 nm copper nanoparticles. ACS Sustain. Chem. Eng. 7:17535-17543. https://doi.org/10.1021/acssuschemeng.9b05078
  23. Molnar Z, Bodai V, Szakacs G, Erdelyi B, Fogarassy Z, Safran G, Varga T, et al. 2018. Green synthesis of gold nanoparticles by thermophilic filamentous fungi. Sci. Rep. 8: 3943. https://doi.org/10.1038/s41598-018-22112-3
  24. Salem MZM, Elansary HO, Ali HM, El-Settawy AA, Elshikh MS, Abdel-Salam EM, et al. 2018. Bioactivity of essential oils extracted from Cupressus macrocarpa branchlets and Corymbia citriodora leaves grown in Egypt. BMC Complement Altern. Med. 18: 23. https://doi.org/10.1186/s12906-018-2085-0
  25. Enan ET, Ashour AA, Basha S, Felemban NH, Gad El-Rab SMF. 2021. Antimicrobial activity of biosynthesized silver nanoparticles, Amoxicillin and glass-ionomer cement against Streptococcus mutans and Staphylococcus aureus. Nanotechnology 32: 215101 (11pp). https://doi.org/10.1088/1361-6528/abe577
  26. Yaqub A, Malkani N, Shabbir A, Ditta S, Tanvir F, Ali S, et al. 2020. Novel biosynthesis of copper nanoparticles using Zingiber and Allium sp. with synergic effect of doxycycline for anticancer and bactericidal activity. Curr. Microbiol. 77:2287-2299. https://doi.org/10.1007/s00284-020-02058-4
  27. Gad El-Rab SMF, Abo-Amer AE, Asiri AM. 2020. Biogenic synthesis of ZnO nanoparticles and its potential use as antimicrobial agent against multidrug-resistant pathogens. Curr. Microbiol. 77:1767-1779. https://doi.org/10.1007/s00284-020-01991-8
  28. Gad El-Rab SMF, Halawani EM, Hassan AM. 2018. Formulation of ceftriaxone conjugated gold nanoparticles and their medical applications against extended-spectrum β-Lactamase producing bacteria and breast cancer. World J. Microbiol. Biotechnol. 28: 1563-1572. https://doi.org/10.1007/s11274-011-0960-7
  29. Dubey SP, Lahtinen M, Sillanpaa M. 2010. Tansy fruit mediated greener synthesis of silver and gold nanoparticles. Process Biochem. 45: 1065-1071. https://doi.org/10.1016/j.procbio.2010.03.024
  30. Halawani EM, Hassan AM, Gad El-Rab SMF. 2020. Nanoformulation of biogenic cefotaxime-conjugated-silver nanoparticles for enhanced antibacterial efficacy against multidrug-resistant bacteria and anticancer studies. Int. J. Nanomed. 5: 1889-1901.
  31. John G. Holt PhD. 1994. Bergey's manual of determinative bacteriology 9th edn (Baltimore, Maryland: Williams & Wilkins) pp. 20: 527-558.
  32. MaccFadin JK. 2000. Biochemical test for identification of medical bacteria 3rd edn (New York: Lippincott Williams and Winkins, AwolterKlumer Company. Philadelphia Baltimore).
  33. Kanmani P, Lim ST. 2013. Synthesis and characterization of pullulan-mediated silver nanoparticles and its antimicrobial activities. Carbohydr. Polym. 12: 421-428. https://doi.org/10.1016/j.carbpol.2013.04.048
  34. Iskandarsyah NH, Rosana Y. 2020. Sinergicity test of silver nanoparticles and clindamycin against Staphylococcus aureus. Int. J. Res. Pharm. Sci. 11: 1192-1198.
  35. Wu S, Rajeshkumar S, Madasamy M, Mahendran V. 2020. Green synthesis of copper nanoparticles using Cissus vitiginea and its antioxidant and antibacterial activity against urinary tract infection pathogens. Artif. Cells Nanomed. Biotechnol. 48: 1153-1158. https://doi.org/10.1080/21691401.2020.1817053
  36. Joseph AT, Prakash P, Narvi S. 2016. Phytofabrication and Characterization of copper nanoparticles using Allium sativum and its antibacterial activity. Int. J. Sci. Eng. Technol. 4: 463-472.
  37. Sengan M, Veerappan A. 2019. N-myristoyltaurine capped copper nanoparticles for selective colorimetric detection of Hg2+ in wastewater and as effective chemocatalyst for organic dye degradation. Microchem. J. 148: 1-9. https://doi.org/10.1016/j.microc.2019.04.049
  38. Suarez-Cerda J, Espinoza-Gomez H, Alonso-Nunez G, et al. 2017. A green synthesis of copper nanoparticles using native cyclodextrins as stabilizing agents. J. Saudi Chem. Soc. 21: 341-348. https://doi.org/10.1016/j.jscs.2016.10.005
  39. Fan D, Zhou Q, Lv X, Jing J, Ye Z, Shao S, Xie J. 2018. Synthesis, thermal conductivity and anti-oxidation properties of copper nanoparticles encapsulated within few-layer h-BN. Ceram. Int. 44:1205-1208. https://doi.org/10.1016/j.ceramint.2017.10.018
  40. Dutta D, Phukan A, Dutta DK. 2018. Nanoporous montmorillonite clay stabilized copper nanoparticles: efficient and reusable catalyst for oxidation of alcohols. Mol. Catal. 451: 178-185. https://doi.org/10.1016/j.mcat.2017.12.032
  41. Gurunathan S, Kalishwaralal K, Vaidyanathan R, Venkataraman D, Pandian SR, Muniyandi J, et al. 2009. Biosynthesis, purification and characterization of silver nanoparticles using Escherichia coli. Colloids Surf. B 74: 328-335. https://doi.org/10.1016/j.colsurfb.2009.07.048
  42. Harraz FM, Hammoda, El-Hawiet A, Radwan MM, Wanas, Eid AME, lSohly MA. 2018. From natural product research chemical constituents, Antibacterial and Acetylcholine esterase inhibitory activity of Cupressus macrocarpa leaves. Nat. Prod. Res. 34: 816-822. https://doi.org/10.1080/14786419.2018.1508140
  43. Fuloria NK, Fuloria S, Chia KY, Karupiah S, Sathasivam K. 2019. Response of green synthesized drug blended silver nanoparticles against periodontal disease triggering pathogenic microbiota. J. Appl. Biol. Biotechnol. 7: 46-56. https://doi.org/10.7324/JABB.2019.70408
  44. Emmanuel R, Palanisamy S, Chen S, Chelladurai K, Padmavathy S, Saravanan M, et al. 2015. Antimicrobial efficacy of green synthesized drug blended silver nanoparticles against dental caries and periodontal disease-causing microorganisms. Mater. Sci. Eng. C 56: 374-379. https://doi.org/10.1016/j.msec.2015.06.033
  45. Li Y, Zhang W, Niu J, Chen Y. 2012. Mechanism of photogenerated reactive oxygen species and correlation with the antibacterial properties of engineered metal-oxide nanoparticles. ACS Nano 6: 5164-5173. https://doi.org/10.1021/nn300934k
  46. Chatterjee AK, Chakraborty R, Basu T. 2014. Mechanism of antibacterial activity of copper nanoparticles. Nanotechnology 25: 135101. https://doi.org/10.1088/0957-4484/25/13/135101
  47. Arumugam A, Karthikeyan C, Haja Hameed AS, Gopinath K, Gowri S, Karthika V. 2015. Synthesis of cerium oxide nanoparticles using Gloriosa superba L. leaf extract and their structural, optical and antibacterial properties. Mater. Sci. Eng. C. 49: 408-415. https://doi.org/10.1016/j.msec.2015.01.042
  48. Mandava K, Kadimcharla K, Keesara NR, Sumayya NF, Prathyusha B, Batchu UR. 2017. Green synthesis of stable copper nanoparticles and synergistic activity with antibiotics. Indian J. Pharm. Sci. 79: 695-700.
  49. Gad El-RabSMF, Halawani EM, Alzahrani SSS. 2021. Biosynthesis of silver nano-drug using Juniperus excelsa and its synergistic antibacterial activity against multidrug-resistant bacteria for wound dressing applications. 3 Biotech 11: 255.
  50. Patel BH, Channiwala MZ, Chaudhari SB, Mandot AA. 2016. Biosynthesis of copper nanoparticles; its characterization and efficacy against human pathogenic bacterium. J. Environ. Chem. Eng. 4: 2163-2169. https://doi.org/10.1016/j.jece.2016.03.046
  51. Hassanien R, Husein DZ, Al-Hakkani MF. 2018. Biosynthesis of copper nanoparticles using aqueous Tilia extract: antimicrobial and anticancer activities. Heliyon 4: e01077. https://doi.org/10.1016/j.heliyon.2018.e01077
  52. Rajeshkumar S, Menon S, Kumar SV, Tambuwala MM, Bakshi H A, Mehta M, et al. 2019. Antibacterial and antioxidant potential of biosynthesized copper nanoparticles mediated through Cissus arnotiana plant extract. J. Photochem. Photobiol. B. 197: 111531. https://doi.org/10.1016/j.jphotobiol.2019.111531
  53. Covarrubias C, Trepiana D, Corral C. 2018. Synthesis of hybrid copper-chitosan nanoparticles with antibacterial activity against cariogenic Streptococcus mutans. Dent. Mater. J. 37: 379-384. https://doi.org/10.4012/dmj.2017-195
  54. Mardones J, Gomez ML, Diaz C, Galleguillos C, Covarrubias C. 2018. In vitro antibacterial properties of copper nanoparticles as endodontic medicament against Enterococcus faecalis. J. Dent. Oral Disord. 4: 1107.