Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Endophytic Fungi of Salt-Tolerant Plants: Diversity and Ability to Promote Plant Growth

  • Khalmuratova, Irina (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Choi, Doo-Ho (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Kim, Jong-Guk (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Lee, In–Seon (Department of Food Science and Technology, Keimyung University)
  • Received : 2021.06.04
  • Accepted : 2021.09.13
  • Published : 2021.11.28
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Abstract

Suaeda australis, Phragmites australis, Suaeda maritima, Suaeda glauca Bunge, and Limonium tetragonum in the Seocheon salt marsh on the west coast of the Korean Penincula were sampled in order to identify the endophytes inhabiting the roots. A total of 128 endophytic fungal isolates belonging to 31 different genera were identified using the fungal internal transcribed spacer (ITS) regions and the 5.8S ribosomal RNA gene. Fusarium, Paraconiothyrium and Alternaria were the most commonly isolated genera in the plant root samples. Various diversity indicators were used to assess the diversity of the isolated fungi. Pure cultures containing each of the 128 endophytic fungi, respectively, were tested for the plant growth-promoting abilities of the fungus on Waito-C rice germinals. The culture filtrate of the isolate Lt-1-3-3 significantly increased the growth of shoots compared to the shoots treated with the control. Lt-1-3-3 culture filtrate was analyzed and showed the presence of gibberellins (GA1 2.487 ng/ml, GA3 2.592 ng/ml, GA9 3.998, and GA24 6.191 ng/ml). The culture filtrate from the Lt-1-3-3 fungal isolate produced greater amounts of GA9 and GA24 than the wild-type Gibberella fujikuroi, a fungus known to produce large amounts of gibberellins. By the molecular analysis, fungal isolate Lt-1-3-3 was identified as Gibberella intermedia, with 100% similarity.

Keywords

Acknowledgement

This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), Ministry of Education (2016R1A6A1A05011910), Research Institute for Dok-do and Ulleung-do Island of Kyungpook National University, and Korea Ministry of Environment as "The Eco-Innovation Project".

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