Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Evaluation of γ-Aminobutyric Acid (GABA) Production by Lactic Acid Bacteria Using 5-L Fermentor

Lactic Acid Bacteria (LAB)와 5-L 발효기를 이용한 γ-Aminobutyric Acid 생산기술 개발

  • Kim, Na Yeon (Department of Food Science and Biotechnology, College of Engineering, Global K-Food Research Center, Hankyong National University) ;
  • Kim, Ji Min (Department of Food Science and Biotechnology, College of Engineering, Global K-Food Research Center, Hankyong National University) ;
  • Ra, Chae Hun (Department of Food Science and Biotechnology, College of Engineering, Global K-Food Research Center, Hankyong National University)
  • 김나연 (한경대학교 식품생명공학과) ;
  • 김지민 (한경대학교 식품생명공학과) ;
  • 라채훈 (한경대학교 식품생명공학과)
  • Received : 2021.07.23
  • Accepted : 2021.11.01
  • Published : 2021.12.28
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Abstract

This study aimed to optimize gamma-aminobutyric acid (GABA) production by employing five strains of lactic acid bacteria (LAB) that were capable of high cell growth and GABA production using a modified synthetic medium. GABA production in the strains was qualitatively confirmed via detection of colored spots using thin layer chromatography. Lactobacillus plantarum SGL058 and Lactococcus lactis SGL027 were selected as the suitable strains for GABA production. The conditions of the carbon and nitrogen sources were determined as 5 g/l glucose (L. plantarum SGL058), 5 g/l lactose (L. lactis SGL027), 10 g/l yeast extract (L. plantarum SGL058), and 20 g/l yeast extract (L. lactis SGL027) for GABA production. The cell growth, monitored by optical density at 600 nm, was 5.93 for L. plantarum SGL058. This value was higher than the 3.04 produced by L. lactis SGL027 at 36 h using a 5-L fermenter. The highest concentration of GABA produced was 546.7 ㎍/ml by L. plantarum SGL058 and 404.6 ㎍/ml by L. lactis SGL027, representing a GABA conversion efficiency of (%, w/w) of 4.0% and 3.4%, respectively. The fermentation profiles of L. plantarum SGL058 and L. lactis SGL027 provide a basis for the utilization of LAB in GABA production using a basal synthetic medium.

유산균 L. plantarum SGL058과 L. lactis SGL027로부터 OFAT 방법을 이용하여 탄소원 및 질소원의 종류와 농도에 대해 실험을 진행하였다. 또한 TLC 분석방법을 통해 GABA 생성능력이 우수한 균주 L. plantarum SGL058과 L. lactis SGL027를 선정하였다. 두 균주를 이용하여 각각 5-L 발효기에서 546.7 ㎍/ml (L. plantarum SGL058)과 404.6 ㎍/ml (L. lactis SGL027)의 GABA를 생산하였다. 이러한 연구결과는 유산균을 이용한 GABA 생산과정에 있어서 유용한 정보를 제공할 것으로 판단된다.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government(MSIT) (No.2019R1G1A1007247).

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