한국자원식물학회지 (Korean Journal of Plant Resources)

  • 제34권6호
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  • Pages.600-607
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  • 2021
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  • 1226-3591(pISSN)
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  • 2287-8203(eISSN)
한국자원식물학회 (The Plant Resources Society of Korea)
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Efficient Cryopreservation of In Vitro Grown Shoot Tips of Strawberry (Fragaria × ananassa Duch.) Germplasm Using Droplet-Vitrification

  • Bae, Jinjoo (National Agrobiodiversity Center, NAS, RDA) ;
  • Lee, Sun-Yi (Department of Horticultural Crop Research, NIHHS, RDA) ;
  • Song, Jae-Young (National Agrobiodiversity Center, NAS, RDA) ;
  • Lee, Jung-Ro (National Agrobiodiversity Center, NAS, RDA) ;
  • Yoon, Munsup (National Agrobiodiversity Center, NAS, RDA) ;
  • Yi, Jung-Yoon (National Agrobiodiversity Center, NAS, RDA) ;
  • Kim, Haeng-Hoon (Department of Well-being Resources, Sunchon National University) ;
  • Lee, Young-Yi (National Agrobiodiversity Center, NAS, RDA)
  • 투고 : 2021.10.31
  • 심사 : 2021.11.15
  • 발행 : 2021.12.01
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초록

Cryopreservation method using a droplet vitrification was applied to the thirty-one strawberry accessions of in vitro grown shoot tips. A protocol with 0.3 - 0.5 M preculture followed by C4 loading and B1 dehydration solutions efficiently implemented cryopreservation of twenty-six strawberry accessions. The highest regrowth rate was 85.8% for PHS0007 and others were ranged between 85.8% and 21.0%. A slightly modified protocol was applied to five accessions. With these two protocols, twenty-eight accessions obtained more than 40% regrowth rate. This study showed that the droplet vitrification method was able to practically implement cryopreservation of in vitro grown shoot tips of broad range of strawberry germplasm (105).

키워드

과제정보

This study was carried out with the support of "Development and application of cryopreservation technique for strawberry and Lilium germplasm and quality management for seed base collection (Project No. PJ014294)", National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

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