Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지
DOI QR코드

DOI QR Code

Immunostimulatory Activity of Hibiscus manihot Flower in Mouse Macrophages, RAW264.7 Cells

  • Geum, Na Gyeong (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Yu, Ju Hyeong (Department of Medicinal Plant Resources, Andong National University) ;
  • Yeo, Joo Ho (Department of Medicinal Plant Resources, Andong National University) ;
  • Choi, Min Yeong (Department of Medicinal Plant Resources, Andong National University) ;
  • Park, Gwang Hun (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Hong, Se Chul (Gumi Electronics & Information Technology Research Institute) ;
  • Jeong, Jin Boo (Department of Medicinal Plant Resources, Andong National University)
  • Received : 2021.10.15
  • Accepted : 2021.11.14
  • Published : 2021.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we investigated whether Hibiscus manihot flowers (HMF) exhibits immunostimulatory activity in RAW264.7 cells. HMF increased the production of immunostimulatory factors such as NO, iNOS, IL-1β and TNF-α in RAW264.7 cells. TLR2 and TLR4 blocked HMF-mediated production of immunostimulatory factors in RAW264.7 cells. In addition, the inhibition of MAPK signaling pathway reduced HMF-mediated production of immunostimulatory factors. From these results, HMF is thought to promote the production of immunostimulatory factors through activating TLR2/4/MAPK signaling in macrophages. It is believed that HMF can be developed as an agent related to immune enhancement in the future.

Keywords

Acknowledgement

Following are results of a study on the "Leaders in INdustry-university Cooperation (LINC)" Project, supported by the Ministry of Education and the National Research Foundation of Korea (NRF).

References

  1. Alagawany, M., Y.A. Attia, M.R. Farag, S.S. Elnesr, S.A. Nagadi, M.E. Shafi, A.F. Khafaga, H. Ohran, A.A. Alaqil and M.E. Abd El-Hack. 2021. The strategy of boosting the immune system under the COVID-19 pandemic. Front. Vet. Sci. 7:570748. https://doi.org/10.3389/fvets.2020.570748
  2. Barbara, J.A., X. Van Ostade and A.F. Lopez. 1996. Tumour necrosis factor-alpha (TNF-α): The good, the bad and potentially very effective. Immunol. Cell Biol. 74:434-443. https://doi.org/10.1038/icb.1996.73
  3. Duque, A.G. and A. Descoteaux. 2014. Macrophage cytokines: Involvement in immunity and infectious diseases. Front. Immunol. 5:491. https://doi.org/10.3389/fimmu.2014.00491
  4. Foley, E. and P.H. O'Farrell. 2003. Nitric oxide contributes to induction of innate immune responses to gram-negative bacteria in Drosophila. Genes Dev. 17:115-125. https://doi.org/10.1101/gad.1018503
  5. Janeway, C.A. Jr. and R. Medzhitov. 2002. Innate immune recognition. Annu. Rev. Immunol. 20:197-216. https://doi.org/10.1146/annurev.immunol.20.083001.084359
  6. Kasimu, R., C. Chen, X. Xie and X. Li. 2017. Water-soluble polysaccharide from Erythronium sibiricum bulb: Structural characterisation and immunomodulating activity. Int. J. Biol. Macromol. 105:452-462. https://doi.org/10.1016/j.ijbiomac.2017.07.060
  7. Kawasaki, T. and T. Kawai. 2014. Toll-like receptor signaling pathways. Front. Immunol. 5:461.
  8. Luan, F., Q. We, Y. Yang, H. Lv, H. Liu, D. Liu, Z. Gan and N. Zeng. 2020. Traditional uses, chemical constituents, biological properties, clinical settings, and toxicities of Abelmoschus manihot L.: A comprehensive review. Front. Pharmacol. 11:1068. https://doi.org/10.3389/fphar.2020.01068
  9. Ma, X.L., M. Meng, L.R. Han, D. Cheng, X.H. Cao and C.L. Wang. 2016. Structural characterization and immunomodulatory activity of Grifola frondosa polysaccharide via toll-like receptor 4-mitogen-activated protein kinases-nuclear factor κb pathways. Food Funct. 7:2763-2772. https://doi.org/10.1039/C6FO00279J
  10. Mills, C.D. and K. Ley. 2014. M1 and M2 macrophages: the chicken and the egg of immunity. J. Innate Immun. 6:716-726. https://doi.org/10.1159/000364945
  11. Mills, C.D., L.L. Lenz and K. Ley. 2015. Macrophages at the fork in the road to health or disease. Front. Immunol. 6:59. https://doi.org/10.3389/fimmu.2015.00059
  12. Nathan, C. and M.U. Shiloh. 2000. Reactive oxygen and nitrogen intermediates in the relationship between mammalian hosts and microbial pathogens. PNASU 97:8841-8848. https://doi.org/10.1073/pnas.97.16.8841
  13. Nick, J.A., N.J. Avdi, P. Gerwins, G.L. Johnson and G.S. Worthen. 1996. Activation of a p38 mitogen-activated protein kinase in human neutrophils by lipopolysaccharide. J. Immunol. 156:4867-4875.
  14. Pan, X.X., J.H. Tao, S. Jiang, Y. Zhu, D.W. Qian and J.A. Duan. 2018. Characterization and immunomodulatory activity of polysaccharides from the stems and leaves of Abelmoschus manihot and a sulfated derivative. Int. J. Biol. Macromol. 107:9-16. https://doi.org/10.1016/j.ijbiomac.2017.08.130
  15. Reiling, N., A. Blumenthal, H.D. Flad, M. Ernst and S. Ehlers. 2001. Mycobacteria-induced TNF-alpha and IL-10 formation by human macrophages is differentially regulated at the level of mitogen-activated protein kinase activity. J. Immunol. 167:3339-3345. https://doi.org/10.4049/jimmunol.167.6.3339
  16. Seillet, C., G.T. Belz and L.A. Mielke. 2014. Complexity of cytokine network regulation of innate lymphoid cells in protective immunity. Cytokine 70:1-10. https://doi.org/10.1016/j.cyto.2014.06.002
  17. Siveen, K.S. and G. Kuttan. 2009. Role of macrophage in tumour progression. Immunol. Lett. 123:97-102. https://doi.org/10.1016/j.imlet.2009.02.011
  18. Tosi, M.F. 2005. Innate immune responses to infection. J. Allergy Clin. Immunol. 116(2):241-249. https://doi.org/10.1016/j.jaci.2005.05.036
  19. Toussi, D.N. and P. Massari. 2014. Immune adjuvant effect of molecularly-defined toll-like receptor ligands. Vaccines 2(2):323-353. https://doi.org/10.3390/vaccines2020323
  20. Wada, T. and J.M. Penninger. 2004. Mitogen-activated protein kinases in apoptosis regulation. Oncogene 23:2838-2849. https://doi.org/10.1038/sj.onc.1207556
  21. Yu, J.H., N.G. Geum, J.H. Yeo and J.B. Jeong. 2021. Immuno-enhancing and anti-obesity effect of Abelmoschus manihot root extracts. Korean J. Plant Res. 34(5):411-419. https://doi.org/10.7732/KJPR.2021.34.5.411
  22. Zhang, C., N. Bai, A. Chang, Z. Zhang, J. Yin, W. Shen, Y. Tian, R. Xiang and C. Liu. 2013. ATF4 is directly recruited by TLR4 signaling and positively regulates TLR4-trigged cytokine production in human monocytes. Cell. Mol. Immunol. 10:84-94. https://doi.org/10.1038/cmi.2012.57