Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Anti-inflammatory Effect of Shea Butter Extracts in Canine Keratinocytes

  • Lim, Dahye (Department of Veterinary Dermatology, College of Veterinary Medicine, Kyungpook National University) ;
  • Bae, Seulgi (Department of Veterinary Dermatology, College of Veterinary Medicine, Kyungpook National University) ;
  • Oh, Taeho (Department of Veterinary Dermatology, College of Veterinary Medicine, Kyungpook National University)
  • Received : 2020.10.13
  • Accepted : 2021.02.09
  • Published : 2021.02.28
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Abstract

Shea butter (Vitellaria paradoxa) is a fat extracted from shea tree nuts and contains relatively high levels of non-glycerides. Triterpenes, the main non-glyceride component, exhibit a variety of biological activities such as antitumor, antibacterial, and anti-inflammatory. Shea butter extract (SBE) has been used to treat various skin problems such as burns, eczema, and rash in human medicine, but little is known about the activity of SBE on canine skin. This study evaluated the cytotoxicity and anti-inflammatory effect of SBE in canine keratinocytes. Cytotoxicity of lipopolysaccharide (LPS, 5-50 ng/mL) and SBE (50-200 ㎍/mL) was evaluated using the CCK-8 assay. Non-cytotoxic concentrations of LPS and SBE were administered to canine cell cultures to evaluate anti-inflammatory effects. To evaluate the anti-inflammatory activity of SBE, the levels of IL-1β, IL-8, IL-12, and TNF-α were measured using ELISA kits. The concentration of each cytokine was quantified in control, LPS-treated, LPS + SBE-treated groups. Increased levels of IL-1β, IL-8, and IL-12 were found in LPS-treated groups relative to control groups. LPS + SBE-treated groups showed a lower level of IL-1β, IL-8, and IL-12 than LPS-treated groups. These results suggest that SBE may have application as a topical agent for canine inflammatory skin diseases. However, further in vivo study is needed to evaluate the safety and efficacy of SBE in dogs.

Keywords

References

  1. Adamu HM, Ushie OA, Nansel E. Antimicrobial activity of oil from Butyrospermum parkii seed (Shea butter). Int J Mod Biol Med 2013; 3: 50-59.
  2. Akihisa T, Kojima N, Katoh N, Kikuchi T, Fukatsu M, Shimizu N, Masters ET. Triacylglycerol and triterpene ester composition of shea nuts from seven African countries. J Oleo Sci 2011; 60: 385-391. https://doi.org/10.5650/jos.60.385
  3. Akihisa T, Kojima N, Kikuchi T, Yasukawa K, Tokuda H, Masters ET, Manosroi A, Manosroi J. Anti-inflammatory and chemopreventive effects of triterpene cinnamates and acetates from shea fat. J Oleo Sci 2010; 59: 273-280. https://doi.org/10.5650/jos.59.273
  4. Alander J. Shea butter-a multifunctional ingredient for food and cosmetics. Lipid Technol 2004; 16: 202-205.
  5. Albanesi C, Scarponi C, Giustizieri ML, Girolomoni G. Keratinocytes in inflammatory skin diseases. Curr Drug Targets: Inflammation Allergy 2005; 4: 329-334. https://doi.org/10.2174/1568010054022033
  6. Andersson AC, Alander J. Shea butter extract for bioactive skin care. C&T 2015; 130: 18-25.
  7. Baroni A, Buommino E, De Gregorio V, Ruocco E, Ruocco V, Wolf R. Structure and function of the epidermis related to barrier properties. Clin Dermatol 2012; 30: 257-262. https://doi.org/10.1016/j.clindermatol.2011.08.007
  8. El-Mahmood AM, Doughari JH, Ladan N. Antimicrobial screening of stem bark extracts of Vitellaria paradoxa against some enteric pathogenic microorganisms. Afr J Pharm Pharmaco 2008; 2: 89-94.
  9. Fernández MA, de las Heras B, Garcia MD, Sáenz MT, Villar A. New insights into the mechanism of action of the anti‐inflammatory triterpene lupeol. J Pharm Pharmacol 2001; 53: 1533-1539. https://doi.org/10.1211/0022357011777909
  10. Frane B, Lemo N. Topical therapy in veterinary dermatology. Vet Stanica 2019; 50: 239-246.
  11. Hall JB, Aebischer DP, Tomlinson HF, Osei-Amaning E, Hindle JR. Vitellaria paradoxa: A monograph. 1996.
  12. Honfo FG, Akissoe N, Linnemann AR, Soumanou M, Van Boekel MA. Nutritional composition of shea products and chemical properties of shea butter: a review. Crit Rev Food Sci Nutr 2014; 54: 673-686. https://doi.org/10.1080/10408398.2011.604142
  13. Israel MO. Effects of topical and dietary use of shea butter on animals. Am J Life Sci 2014; 2: 303-307. https://doi.org/10.11648/j.ajls.20140205.18
  14. Kondo S. The roles of keratinocyte-derived cytokines in the epidermis and their possible responses to UVA-irradiation. J Investig Dermatol Symp Proc 1999; 4: 177-183. https://doi.org/10.1038/sj.jidsp.5640205
  15. Lin TK, Zhong L, Santiago JL. Anti-inflammatory and skin barrier repair effects of topical application of some plant oils. Int J Mol Sci 2018; 19: 70. https://doi.org/10.3390/ijms19010070
  16. Loden M, Andersson AC. Effect of topically applied lipids on surfactant‐irritated skin. Br J Dermatol 1996; 134: 215-220. https://doi.org/10.1046/j.1365-2133.1996.978714.x
  17. Luger TA, Schwarz T, Krutmann J, Kirnbauer R, Neuner P, Köck A, Urbanski A, Borth W, Schauer E. Interleukin‐6 is produced by epidermal cells and plays an important role in the activation of human T‐lymphocytes and natural killer cells. Ann NY Acad Sci 1989; 557: 405-414.
  18. Maranz S, Kpikpi W, Wiesman Z, De Saint Sauveur A, Chapagain B. Nutritional values and indigenous preferences for shea fruits (Vitellaria paradoxa CF Gaertn. F.) in African agroforestry parklands. Econ Bot 2004; 58: 588-600. https://doi.org/10.1663/0013-0001(2004)058[0588:NVAIPF]2.0.CO;2
  19. Maranz S, Wiesman Z. Influence of climate on the tocopherol content of shea butter. J Agric Food Chem 2004; 52: 2934-2937. https://doi.org/10.1021/jf035194r
  20. Mckenzie RC, Sauder DN. The role of keratinocyte cytokines in inflammation and immunity. J Invest Dermatol 1990; 95: S105-107. https://doi.org/10.1111/1523-1747.ep12874955
  21. Miller Jr WH, Griffin CE, Campbell KL. Muller and Kirk's small animal dermatology. 7th ed. Elsevier Health Sciences. 2012: 114-116.
  22. Peers KE. The non‐glyceride saponifiables of shea butter. J Sci Food Agric 1977; 28: 1000-1009. https://doi.org/10.1002/jsfa.2740281109
  23. Rosenkrantz W. Practical applications of topical therapy for allergic, infectious, and seborrheic disorders. Clin Tech Small Anim Pract 2006; 21: 106-116. https://doi.org/10.1053/j.ctsap.2006.05.003
  24. Sauder DN. The role of epidermal cytokines in inflammatory skin diseases. J Invest Dermatol 1990; 95.
  25. Siddique HR, Saleem M. Beneficial health effects of lupeol triterpene: a review of preclinical studies. Life Sci 2011; 88: 285-293. https://doi.org/10.1016/j.lfs.2010.11.020
  26. Sims J, Towne J, Blumberg H. IL-1 family members in inflammatory skin disease. Proceedings of the ernst schering foundation symphosium. 2005: 187-191.
  27. Slominski AT, Zmijewski MA, Skobowiat C, Zbytek B, Slominski RM, Steketee JD. Sensing the environment: Regulation of local and global homeostasis by the skin neuroendocrine system. Adv Anat Embryol Cell Biol 2012; 212: v-115.
  28. Suter MM, Schulze K, Bergman W, Welle M, Roosje P, Müller EJ. The keratinocyte in epidermal renewal and defence. Vet Dermatol 2009; 20: 515-532. https://doi.org/10.1111/j.1365-3164.2009.00819.x
  29. Tabassum N, Hamdani M. Plants used to treat skin diseases. Pharmacogn Rev 2014; 8: 52. https://doi.org/10.4103/0973-7847.125531
  30. Verma N, Chakrabarti R, Das RH, Gautam HK. Anti-inflammatory effects of shea butter through inhibition of iNOS, COX-2, and cytokines via the Nf-Kb pathway in LPS-activated J774 macrophage cells. J Altern Complement Med 2012; 9: 1-11. https://doi.org/10.1089/107555303321222856

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