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Analysis on the Efficacy of Cosmetic Application of Lijang Snow Tea (Nekemias grossedentata)

리장 설차 (Nekemias grossedentata )의 화장품적 적용 효능 분석

  • Wen, Ying (Department of Health and Cosmetics, Dongduk Women's University) ;
  • Lee, Seol-Hoon (Division of Applied Chemistry and Cosmetic Science, Dongduk Women's University)
  • 문영 (동덕여자대학교 보건향장대학원) ;
  • 이설훈 (동덕여자대학교 화학-화장품 학부)
  • Received : 2021.10.07
  • Accepted : 2021.11.06
  • Published : 2021.12.30

Abstract

In this study, we analyzed the cosmetic applicability of extract from snow tea, native to Lijiang, Yunnan-province, China. After confirming the species as N. grossedentata through DNA analysis of Lijiang snow tea, experiments were conducted using representative tea, green tea, and a representative control group for each efficacy analysis. Both teas were extracted using 70% (v/v) ethanol aqueous solution. The polyphenol content in the Lijiang snow tea extract (gallic acid equivalent, 23.9 ± 3.2 mg/mL) was higher than that in green tea extract (16.4 ± 2.3 mg/mL). In contrast, the antioxidant (Radical scavenging, IC50 104 ㎍/mL), tyrosinase enzyme inhibitory (whitening agent, IC50 40.7 ㎍/mL), and Escherichia coli growth inhibitory (preservative) activities (IC50 2.85 mg/mL) were analyzed based on the solid content in the extract, and it was confirmed that the activities of Lijiang snow tea extract were superior to those of green tea extract (radical scavenging, IC50 234 ㎍/mL. It also showed similar efficacy to previously used active substances such as antioxidants (vitamin C, IC50 108 ㎍/mL), whitening agents (vitamin C, IC50 80㎍/mL), and preservatives (methylparaben, IC50 4.35 mg/mL). However, green tea was found to be better in collagenase inhibition activity (anti-wrinkle). Through this study, the cosmetic application potential of Lijiang snow tea is high.

중국 운남성 리장지방에 자생하는 리장설차의 화장품적 적용 가능성을 분석 하였다. 우선 리장 설차의 DNA 분석을 통해서 Nekemias grossedentata (N. grossedentata) 라는 종을 확인한 후, 대표적인 차인 녹차와 각 효능분석에 대표적인 대조군을 사용하여 실험하였다. 먼저 리장 설차는 70% (v/v)에탄올 수용액으로 추출하였다. 리장설차 추출물내의 폴리 페놀함량 (gallic acid equivalent, 23.9 ± 3.2 mg/mL)은 녹차(16.4 ± 2.3 mg/mL)보다 많은 양을 포함 하고 있었다. 한편 추출물내의 고형분을 기준으로 항산화 활성(라디칼소거, IC50 104 ㎍/mL), Tyrosinase 효소 억제 활성(미백, IC50 40.7 ㎍/mL), 대장균 성장 억제 활성(방부: IC50 2.85 mg/mL)을 분석한 결과 녹차(항산화, 라디칼 소거, IC50 234 ㎍/mL) 보다 우수한 것 확인 하였다. 또한 항산화 (비타민C, IC50 108 ㎍/mL), 미백 (비타민C: IC50 80 ㎍/mL), 방부(메틸 파라벤: IC50 4.35 mg/mL)등의 기존에 사용되던 활성물질과도 유사한 효능을 보여 주었. 그러나 콜라게네이즈 억제 활성(주름) 은 녹차가 더 우수한 것으로 나타났다. 이를 통해 리장 설차의 화장품적 적용 가능성이 높은 것을 확인할 수 있었다.

Keywords

Acknowledgement

This thesis was supported by the Dongduk Women's University Grant.

References

  1. H. Luo, M. Ren, K. M. Lim, Y. J. Koh, L. S. Wang, and J. S. Hur, Antioxidative activity of lichen Thamnolia vermicularis in vitro, Mycobiology, 34(3), 124 (2006). https://doi.org/10.4489/MYCO.2006.34.3.124
  2. J. M. Lord, A. Knight, J. M. Bannister, L. R. Ludwig, W. M. Malcolm, and D. A. Orlovich, Rediscovery of pycnidia in Thamnolia vermicularis: implications for chemotype occurrence and distribution, Lichenologist, 45(3), 397 (2013). https://doi.org/10.1017/s0024282913000017
  3. Y. Zhao, M. Wang, and B. Xu, A comprehensive review on secondary metabolites and health-promoting effects of edible lichen, J. Funct. Foods, 80, 104283 (2021). https://doi.org/10.1016/j.jff.2020.104283
  4. J. Guo, Z. Li, A. Wang, X. Liu, J. Wang, X. Guo, Y. Jing, and H. Hua, Three new phenolic compounds from the lichen Thamnolia vermicularis and their antiproliferative effects in prostate cancer cells, Planta Med, 77(18), 2042 (2011). https://doi.org/10.1055/s-0031-1280096
  5. R. Y. Choi, J. R. Ham, J. Yeo, J. S. Hur, S. K. Park, M. J. Kim, and M. K. Lee, Anti-obesity property of lichen Thamnolia vermicularis extract in 3T3-L1 cells and diet-induced obese mice, Prev. Nutr. Food Sci, 22(4), 285 (2017). https://doi.org/10.3746/pnf.2017.22.4.285
  6. V. Pant and P. B. Rao, Antioxidant and GC-MS analysis of Thamnolia subuliformis (Ehrh.) W.L. Culb. from western Himalaya, The Pharma Innovation Journal, 7(12), 82 (2018). https://doi.org/10.7897/2277-4572.07386
  7. Y. U. Haiyuan, X. Shen, D. Liu, M. Hong, and Y. Lu, The protective effects of β-sitosterol and vermicularin from Thamnolia vermicularis (Sw.) Ach. against skin aging in vitro, An. Acad. Bras. Cienc., 91(4), 11 (2019).
  8. S. P. J. Namal Senanayake, Green tea extract: chemistry, antioxidant properties and food applications - a review, J. Funct. Foods, 5(4), 1529 (2013). https://doi.org/10.1016/j.jff.2013.08.011
  9. M. D. Gianeti, D. G. Mercurio, and P. M. B. G. Maia Campos, The use of green tea extract in cosmetic formulations: not only an antioxidant active ingredient, Dermatol. Ther., 26(3), 267 (2013). https://doi.org/10.1111/j.1529-8019.2013.01552.x
  10. S. Verdier-Sevrain and F. Bonte, Skin hydration: a review on its molecular mechanisms, J. Cosmet. Dermatol., 6(2), 75 (2007). https://doi.org/10.1111/j.1473-2165.2007.00300.x
  11. H. J. Kim, S. Kim, and S. H. Lee, Non-invasive skin barrier lipid packing analysis using FT-IR and study of cosmetic formulation for damaged barrier, J. Soc. Cosmet. Sci. Korea, 46(3), 307 (2020). https://doi.org/10.15230/SCSK.2020.46.3.307
  12. O. V. Zillich, U. Schweiggert-Weisz, P. Eisner, and M. Kerscher, Polyphenols as active ingredients for cosmetic products, Int. J. Cosmet. Sci., 37(5), 455 (2015). https://doi.org/10.1111/ics.12218
  13. K. U. Schallreuter, S. Kothari, B. Chavan, and J. D. Spencer, Regulation of melanogenesis - controversies and new concepts, Exp. Dermatol., 17(5), 395 (2008). https://doi.org/10.1111/j.1600-0625.2007.00675.x
  14. S. H. Lee, S. H. Jun, J. Yeom, s. G. Park, C. K. Lee, and N. G. Kang, Optical clearing agent reduces scattering of light by the stratum corneum and modulates the physical properties of coenocytes via hydration, Ski. Res. Technol., 24(3), 371 (2018). https://doi.org/10.1111/srt.12439
  15. Y. Takema, M. Hattori, and K. Aizawa, The relationship between quantitative changes in collagen and formation of wrinkles on hairless mouse skin after chronic UV irradiation, J. Dermatol. Sci., 12(1), 56 (1996). https://doi.org/10.1016/0923-1811(95)00467-X
  16. R. Campana, C. Scesa, V. Patrone, E. Vittoria, and W. Baffone, Microbiological study of cosmetic products during their use by consumers: health risk and efficacy of preservative systems, Lett. Appl. Microbiol. 43(3), 301 (2006). https://doi.org/10.1111/j.1472-765X.2006.01952.x
  17. O. Folin and W. Denis, On phosphotungstic-phoshomolybdic compunds as color reagents, J. Biol. Chem., 12(2), 239 (1912). https://doi.org/10.1016/S0021-9258(18)88697-5
  18. S. I. Liochev, Reactive oxygen species and the free radical theory of aging, Free Radic. Biol. Med., 60, 1 (2013). https://doi.org/10.1016/j.freeradbiomed.2013.02.011
  19. T. Pillaiyar, M. Manickam, and V. Namasivayam, Skin whitening agents: medicinal chemistry perspective of tyrosinase inhibitors, J. Enzyme Inhib. Med. Chem., 32(1), 403 (2017). https://doi.org/10.1080/14756366.2016.1256882
  20. U. Panich, V. Tangsupa-a-nan, T. Onkoksoong, K. Kongtaphan, K. Kasetsinsombat, P. Akarasereenont, and A. Wongkajornsilp, Inhibition of UVA-mediated melanogenesis by ascorbic acid through modulation of antioxidant defense and nitric oxide system, Arch. Pharm. Res., 34(5), 811 (2011). https://doi.org/10.1007/s12272-011-0515-3
  21. N. Taira, Y. Katsuyama, M. Yoshioka, O. Muraoka, and T. Morikawa, Structural requirements of alkylglyceryll-ascorbic acid derivatives for melanogenesis inhibitory activity, Int. J. Mol. Sci., 19(4), 1144 (2018). https://doi.org/10.3390/ijms19041144
  22. A. K. Langton, M. J. Sherratt, C. E. M. Griffiths, and R. E. B. Watson, A new wrinkle on old skin: the role of elastic fibres in skin ageing, Int. J. Cosmet. Sci., 32(5), 330 (2010). https://doi.org/10.1111/j.1468-2494.2010.00574.x
  23. K. S. Kang, I. D. Kim, R. H. Kwon, Y. Y. Heo, S. H. Oh, M. A. Kim, H. J. Jung, H. Y. Kang, and B. J. Ha, The evaluation of anti-wrinkle effects in oriental herb extract, J. Life Sci., 17(8), 1147 (2007). https://doi.org/10.5352/JLS.2007.17.8.1147
  24. M. Sazuka, H. Imazawa, Y. Shoji, T. Mita, Y. Hara, and M. Isemura, Inhibition of collagenases from mouse lung carcinoma cells by green tea catechins and black tea theaflavins, Biosci. Biotechnol. Biochem., 61(9), 1504 (1997). https://doi.org/10.1271/bbb.61.1504
  25. M. G. Soni, S. L. Taylor, N . A. Greenberg, and G. A. Burdock, Evaluation of the health aspects of methyl paraben: a review of the published literature, Food Chem. Toxicol., 40(10), 1335 (2002). https://doi.org/10.1016/S0278-6915(02)00107-2