한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제44권4호
  • /
  • Pages.247-256
  • /
  • 2021
  • /
  • 3022-7372(pISSN)
  • /
  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
권호 표지
DOI QR코드

DOI QR Code

재조합 lysozyme-HJP34 단백질의 다양한 병원성 세균에 대한 항균 효능 및 자돈 사료첨가제의 가능성 평가

Antibacterial evaluation of recombinant lysozyme-HJP24 proteins against various bacterial pathogens, and of its possibility test as a feed additive in piglets

  • 유정희 (전북대학교 수의과대학 수의공중보건학실) ;
  • 유영주 (전북대학교 수의과대학 수의공중보건학실) ;
  • 김선민 (전북대학교 수의과대학 수의공중보건학실) ;
  • 허진 (전북대학교 수의과대학 수의공중보건학실)
  • Yu, Jeong-Hee (Department of Veterinary Public Health, College of Veterinary Medicine, Jeonbuk National University) ;
  • Yu, Yeong-Ju (Department of Veterinary Public Health, College of Veterinary Medicine, Jeonbuk National University) ;
  • Kim, Seon-Min (Department of Veterinary Public Health, College of Veterinary Medicine, Jeonbuk National University) ;
  • Hur, Jin (Department of Veterinary Public Health, College of Veterinary Medicine, Jeonbuk National University)
  • 투고 : 2021.12.12
  • 심사 : 2021.12.28
  • 발행 : 2021.12.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The recombinant lysozyme-HJL34 proteins were expressed and purified using commercial Escherichia (E.) coli expression system. Stx2e+ F18+ E. coli, Actinobacillus pleuropneumoniae (APP), Streptococcus (S.) suis, and Clostridium (C.) perfringens strains were isolated from pigs. The minimum inhibitory concentrations (MICs) of the recombinant lysozyme-HJP34 proteins were examined by means of the microtiter plate method, according to the NCCLS recommendations. The possibility of its as the alternatives to antibiotics was tested in piglets. The MICs were determined as 75 ㎍/mL, 300 ㎍/mL, 75 ㎍/mL, 35.5 ㎍/m against Stx2e+ F18+ E. coli, APP, S. suis, C. perfringens, respectively. A total of 25 piglets were divied 5 groups. The piglets in group A~C were fed with commercial feed and those in groups D, E were fed with commercial feedstuff. All piglets in groups B~E were challenged with virulent Stx2e+ F18+ E. coli, APP, S. suis strains. Groups C and D were treated with antimicrobial from 24 h after challenge. All piglets in group B died within 3 days after challenge. Among 5 piglets in groups C and D piglets, 80% survived after challenge. Among group E piglets, 60% were alive until the end of this study. Therefore, this study indicates that recombinant lysozyme-HJP34 proteins is a suitable possibility as a feed additive for reduction of diseases by bacterial pathogens in piglet feed.

키워드

과제정보

이 논문은 정부의 재원으로 농림식품기술기획평가원(Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry, IPET)의 지원을 받아 연구되었습니다(No. 320070-2).

참고문헌

  1. Boman HG. 1995. Peptide antibiotics and their role in innate immunity. Annu Rev Immunol 13: 61-92. https://doi.org/10.1146/annurev.iy.13.040195.000425
  2. Boman HG. 2003. Antibacterial peptides: basic facts and emerging concepts. J Intern Med 254(3): 197-215. https://doi.org/10.1046/j.1365-2796.2003.01228.x
  3. Bosse JT, Lia Y, Angenb O, Weinertc LA, Chaudhuric RR, Holdend MT, Williamsone SM, Maskelld DJ, Tuckerd AW, Wrenf BW, Rycroftg AN, Langforda PR, BRaDP1T consortium. 2014. Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12. Clin Microbiol 52(7): 2380-2385. https://doi.org/10.1128/JCM.00685-14
  4. Bulet P, Stocklin R, Menin L. 2004. Anti-microbial peptides: From invertebrates to vertebrates. Immunol Rev 198: 169-184. https://doi.org/10.1111/j.0105-2896.2004.0124.x
  5. Carballar-Lejarazu R, Rodriguez MH, de la Cruz Hernandez-Hernandez F, Ramos-Castaneda J, Possani LD, Zurita-Ortega M, Reynaud-Garza E, Hernandez-Rivas R, Loukeris T, Lycett G, Lanz-Mendoza H. 2008. Recombinant scorpine: a multifunctional antimicrobial peptide with activity against different pathogens. Cell Mol Life Sci 65(19): 3081-3092. https://doi.org/10.1007/s00018-008-8250-8
  6. Clarke TB, Davis KM, Lysenko ES, Zhou AY, Yu Y, Weiser JN. 2010. Recognition of peptidoglycan from the microbiota by Nod1 enhances systemic innate immunity. Nat Med 16:228-231. https://doi.org/10.1038/nm.2087
  7. Cooper DM, Swanson DL, Gebhart CJ. 1997. Diagnosis of proliferative enteritis in frozen and formalin-fixed, paraffin-embedded tissues from a hamster, horse, deer and ostrich using a Lawsonia intracellularis-specific multiplex PCR assay. Vet Microbiol 54(1): 47-62. https://doi.org/10.1016/S0378-1135(96)01264-3
  8. Ellison III RT, Giehl TJ. 1991. Killing of gram-negative bacteria by lactoferrin and lysozyme. J Clin Invest 88: 1080-1091. https://doi.org/10.1172/JCI115407
  9. Fratini F, Cilia G, Turchi B, Felicioli A. 2017. Insects, arachnids and centipedes venom: A powerful weapon against bacteria. A literature review. Toxicon 130: 91-103. https://doi.org/10.1016/j.toxicon.2017.02.020
  10. Haines LR, Thomas JM, Jackson AM, Eyford BA, Razavi M, Watson CN, Gowen B, Hancock RE, Pearson TW. 2009. Killing of trypanosomatid parasites by a modified bovine host defense peptide, BMAP-18. PLoS Negl Trop Dis 3(2): e373. https://doi.org/10.1371/journal.pntd.0000373
  11. Han SM, Hong IP, Woo SO, Kim SG, Jang HR. 2015. Analysis of bee venom residues in milks of dairy cattle using UHPLC with newly developed pre-processing method. Korean J Vet Serv 38(1): 25-30. https://doi.org/10.7853/KJVS.2015.38.1.25
  12. Han SM, Kim JM, Yeo JH, Hong IP, Woo SO, Lee KG, Kweon HY. 2014. Origin and effective ingredient standards of honeybee venom as natural antibiotic ingredients. Korean J Vet Serv 37(2): 123-129. https://doi.org/10.7853/KJVS.2014.37.2.123
  13. Han SM, Kim SG, Hong IP, WooSO, Jang HR, Lee KW. 2016. Antibacterial effects of purified bee venom against some pathogenic bacteria isolated from dead chickens. Korean J Vet Serv 39(3): 159-166. https://doi.org/10.7853/KJVS.2016.39.3.159
  14. Hays VW, Muir WM. 1979. Efficiency and safety of feed additive use of antibacterial drugs in animal production. Can J Anim Sci 59: 447-456. https://doi.org/10.4141/cjas79-055
  15. Henzler-Wildman KA, Martinez GV, Brown MF, Ramamoorthy A. 2004. Perturbation of the hydrophobic core of lipid bilayers by the human antimicrobial peptide LL-37. Biochemistry 43(26): 8459-8469. https://doi.org/10.1021/bi036284s
  16. Jeong JW, Jeong CW, Kim JT, Yang WJ, Ahn MJ, Kim BH, Kim JA, Shin T. 2009. Antibacterial activity of Callophyllis japonica-methanol extracts against the pathogenic bacteria from swine. Korean J Vet Serv 32(4): 353-359.
  17. Jessing SG, Angen O, Inzana TJ. 2003. Evaluation of a multiplex PCR test for simultaneous identification and serotyping of Actinobacillus pleuropneumoniae serotypes 2, 5 and 6. J Clin Microbiol 41(9): 4095-4100. https://doi.org/10.1128/JCM.41.9.4095-4100.2003
  18. Jung S, Oh SI, Lee HG, Jung YH, Hur TY, Han S, Baek KJ, Cho A. 2021. Antibacterial effect of bee venom against Gram-positive and negative bacteria isolated from mastitis in dairy cattle. Korean J Vet Serv 44(3): 169-174. https://doi.org/10.7853/KJVS.2021.44.3.169
  19. Kawano M, Namba Y, Hanaoka M. 1981. Regulatory factors of lymphocyte-lymphocyte interaction. I. Con A-induced mitogenic factor acts on the late G1 stage of T-cell proliferation. Microbiol Immunol 25: 505-515. https://doi.org/10.1111/j.1348-0421.1981.tb00052.x
  20. Kim ST, Kim S, Kim SY, Son JK. 1997. Comparison of fatty acid composition of Staphylococcus sp. isolated from bovine mastitis milk. Korean J Vet Serv 20(1): 37-45.
  21. Koehbach J, Craik DJ. 2019. The vast structural diversity of antimicrobial peptides. Trends Pharmacol Sci 40(7): 517-528. https://doi.org/10.1016/j.tips.2019.04.012
  22. Kuhn-Nentwig, L. 2003. Antimicrobial and cytolytic peptides of venomous arthropods. Cell Mol Life Sci 60: 2651-2668. https://doi.org/10.1007/s00018-003-3106-8
  23. Kwon AJ, Moon JY, Kim WK, Kim S, Hur J. 2016. Protection efficacy of the Brucella abortus ghost vaccine candidate lysed by the N-terminal 24-amino acid fragment (GI24) of the 36-amino acid peptide PMAP-36 (porcine myeloid antimicrobial peptide 36) in murine models. J Vet Med Sci 78(10): 1541-1548. https://doi.org/10.1292/jvms.16-0036
  24. Lee WW, Son SK, Lee GR, Kim GH, Kim YH. 2011. Antimicrobial effects of garlic extract against pathogenic bacteria. Korean J Vet Serv 34(2): 167-178. https://doi.org/10.7853/KJVS.2011.34.2.167
  25. Oliver WT, Wells JE. 2015. Lysozyme as an alternative to growth promoting antibiotics in swine production. J Anim Sci Biotechnol 6(1): 35. https://doi.org/10.1186/s40104-015-0034-z
  26. Salmon SA, Watts JL, Case CA, Horrman LJ, Wegener HC, Yancey RJ Jr. 1995. Comparison of MICs of ceftiofur and other antimicrobial agents against bacterial pathogens of swine from the united states, canada, and denmark. J Clin Microbiol 33(9): 2435-2444. https://doi.org/10.1128/jcm.33.9.2435-2444.1995
  27. Theppangna W, Murase T, Tokumaru N, Chikumi K, Shimizu E, Otsuki K. 2007. Screening of the enterocin genes and antimicrobial activity against pathogenic bacteria in Enterococcus strains obtained from different origins. J Vet Med Sci 69(12): 1235-1239. https://doi.org/10.1292/jvms.69.1235
  28. Verstegen MW, Williams BA. 2002. Alternatives to the use of antibiotics as growth promoters for monogastric animals. Anim Biotechnol 13(1): 113-127. https://doi.org/10.1081/ABIO-120005774
  29. Vila-Farres X, Garcia de la Maria C, Lopez-Rojas R, Pachon J, Giralt E, Vila J. 2012. In vitro activity of several antimicrobial peptides against colistin-susceptible and colistin-resistant Acinetobacter baumannii. Clin Microbiol Infect 18(4): 383-387. https://doi.org/10.1111/j.1469-0691.2011.03581.x
  30. Wang K, Li Y, Xia Y, Liu C. 2016. Research on peptide toxins with antimicrobial activities. Ann Pharmacol Pharm 1: 1006.
  31. Wells JE, Berry ED, Kalchayanand N, Rempel LA, Kim M, Oliver WT. 2015. Effect of lysozyme or antibiotics on faecal zoonotic pathogens in nursery pigs. J Appl Microbiol 118(6): 1489-1497. https://doi.org/10.1111/jam.12803
  32. Wilmes M, Sahl HG. 2014. Defensin-based anti-infective strategies. Int J Med Microbiol 304(1): 93-99. https://doi.org/10.1016/j.ijmm.2013.08.007
  33. Yeung AT, Gellatly SL, Hancock RE. 2011. Multifunctional cationic host defence peptides and their clinical applications. Cell Mol Life Sci 68: 2161-2176. https://doi.org/10.1007/s00018-011-0710-x