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Prevalence and pathologic study of porcine epidemic diarrhea in Jeju

제주도의 돼지유행성설사 발생 상황과 병리학적 고찰

  • Kim, Hyeon-Ju (Jeju Self-Governing Provincial Veterinary Research Institute) ;
  • Yang, Hyoung-Seok (Jeju Self-Governing Provincial Veterinary Research Institute) ;
  • Kang, Sang Chul (Optipharm Inc.) ;
  • Kim, Jae-Hoon (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
  • 김현주 (제주특별자치도 동물위생시험소) ;
  • 양형석 (제주특별자치도 동물위생시험소) ;
  • 강상철 (옵티팜) ;
  • 김재훈 (제주대학교 수의과대학 및 수의과학연구소)
  • Received : 2021.06.09
  • Accepted : 2021.08.02
  • Published : 2021.12.31

Abstract

From April 2014 to September 2015, 153 piglets from 52 farms in Jeju were diagnosed with porcine epidemic diarrhea (PED). The major PED cases were focused on suckling piglets (144 piglets, 94.1%), particularly in 1-7-day-old piglets. Histopathologically, severe villous atrophy was observed in the small intestine, especially in the jejunum and ileum. The mean villous height to crypt depth ratios of the jejunum and ileum were 1.4:1 and 1.5:1, respectively. The major histopathologic findings of the small intestine were cytoplasmic vacuolation, cuboidalization, squamation, and exfoliation of the mucosal enterocytes in the villi. The cytoplasmic vacuolations in the enterocytes were the most prevalent lesions in the small intestine and were more severe in the ileum than in the jejunum. According to immunohistochemistry methods, the PED virus (PEDV) antigens were presented in the cytoplasms of the enterocytes, and were distributed more prevalently in the ileum than in the jejunum. PEDV antigens were also detected in the colon of 26 piglets (19.5%). Sequence comparison and phylogenetic analysis indicated that 12 PEDV had more than a 98.9% homology with each other. These PEDV strains were highly homologous with the genogroup 2 North American group.

Keywords

Acknowledgement

본 논문은 농림축산검역본부 학술연구용역과제(Z-1543083-2017-18-01) 연구비를 지원받아 수행되었으며, 이에 감사드립니다.

References

  1. Okda F, Liu X, Singrey A, Clement T, Nelson J, Christopher-Hennings J, Nelson EA, Lawson S. Development of an indirect ELISA, blocking ELISA, fluorescent microsphere immunoassay and fluorescent focus neutralization assay for serologic evaluation of exposure to North American strains of Porcine Epidemic Diarrhea Virus. BMC Vet Res 2015;11:180. https://doi.org/10.1186/s12917-015-0500-z
  2. Pensaert MB, Yeo SG. Porcine Epidemic Diarrhea. In: Straw BE, Zimmermann JJ, D'Allaire S, Taylor DJ, eds. Diseases of Swine. 9th ed. pp. 367-372, Blackwell Publishing, Ames, 2006.
  3. Takahashi K, Okada K, Ohshima K. An outbreak of swine diarrhea of a new-type associated with coronavirus-like particles in Japan. Nihon Juigaku Zasshi 1983;45:829-832. https://doi.org/10.1292/jvms1939.45.829
  4. Kweon CH, Kwon BJ, Jung TS, Kee YJ, Hur DH, Hwang EK, Rhee JC, An SH. Isolation of porcine epidemic diarrhea virus (PEDV) in Korea. Korean J Vet Res 1993;33:249-254.
  5. Hwang EK, Kim JH, Jean YH, Bae YC, Yoon SS, Park CK, Kweon CH, Yoon YD, Ackermann M. Current occurrence of porcine epidemic diarrhoea in Korea. RDA J Agric Sci 1994;36:587-596.
  6. Jung K, Wang Q, Scheuer KA, Lu Z, Zhang Y, Saif LJ. Pathology of US porcine epidemic diarrhea virus strain PC21A in gnotobiotic pigs. Emerg Infect Dis 2014;20:662-665. https://doi.org/10.3201/eid2004.131685
  7. Lee S, Ko DH, Kwak SK, Lim CH, Moon SU, Lee DS, Lee C. Reemergence of porcine epidemic diarrhea virus on Jeju Island. Korean J Vet Res 2014;54:185-188. https://doi.org/10.14405/kjvr.2014.54.3.185
  8. Jin W, Kim YH, Han JH. Effects of porcine circovirus type 2 on the pathogenesis of porcine epidemic diarrhoea virus infection in piglets: II. Clinical sign, histopathological lesion and immunohistochemical finding. Korean J Vet Serv 2009;32:189-200.
  9. Chen Q, Li G, Stasko J, Thomas JT, Stensland WR, Pillatzki AE, Gauger PC, Schwartz KJ, Madson D, Yoon KJ, Stevenson GW, Burrough ER, Harmon KM, Main RG, Zhang J. Isolation and characterization of porcine epidemic diarrhea viruses associated with the 2013 disease outbreak among swine in the United States. J Clin Microbiol 2014;52:234-243. https://doi.org/10.1128/JCM.02820-13
  10. Cho YY, Lim SI, Kim YK, Song JY, Lee JB, An DJ. Complete Genome Sequence of K14JB01, a Novel Variant Strain of Porcine Epidemic Diarrhea Virus in South Korea. Genome Announc 2014;2:e00505-e00514.
  11. Vlasova AN, Marthaler D, Wang Q, Culhane MR, Rossow KD, Rovira A, Collins J, Saif LJ. Distinct characteristics and complex evolution of PEDV strains, North America, May 2013-February 2014. Emerg Infect Dis 2014;20:1620-1628. https://doi.org/10.3201/eid2010.140491
  12. Park CK, Lee CH. Clinical examination and control measures in a commercial pig farm persistently infected with porcine epidemic diarrhea (PED) virus. J Vet Clin 2009;26:463-466.
  13. Kang WC, Kim JH, Ahn MJ, Kang JC. Immunohistochemical study of causative agents for the porcine viral diarrhea disease on newborn piglets in Cheju. Korean J Vet Serv 2000;23:301-306.
  14. Kim O, Chae C. Experimental infection of piglets with a Korean strain of porcine epidemic diarrhoea virus. J Comp Pathol 2003;129:55-60. https://doi.org/10.1016/S0021-9975(02)00170-6
  15. Sueyoshi M, Tsuda T, Yamazaki K, Yoshida K, Nakazawa M, Sato K, Minami T, Iwashita K, Watanabe M, Suzuki Y, et al. An immunohistochemical investigation of porcine epidemic diarrhoea. J Comp Pathol 1995;113:59-67. https://doi.org/10.1016/S0021-9975(05)80069-6
  16. Sun RQ, Cai RJ, Chen YQ, Liang PS, Chen DK, Song CX. Outbreak of porcine epidemic diarrhea in suckling piglets, China. Emerg Infect Dis 2012;18:161-163. https://doi.org/10.3201/eid1801.111259
  17. Kim SH, Lee JM, Jung J, Kim IJ, Hyun BH, Kim HI, Park CK, Oem JK, Kim YH, Lee MH, Lee KK. Genetic characterization of porcine epidemic diarrhea virus in Korea from 1998 to 2013. Arch Virol 2015;160:1055-1064. https://doi.org/10.1007/s00705-015-2353-y
  18. Stevenson GW, Hoang H, Schwartz KJ, Burrough ER, Sun D, Madson D, Cooper VL, Pillatzki A, Gauger P, Schmitt BJ, Koster LG, Killian ML, Yoon KJ. Emergence of Porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences. J Vet Diagn Invest 2013;25:649-654. https://doi.org/10.1177/1040638713501675
  19. Zhang X, Pan Y, Wang D, Tian X, Song Y, Cao Y. Identification and pathogenicity of a variant porcine epidemic diarrhea virus field strain with reduced virulence. Virol J 2015;12:88. https://doi.org/10.1186/s12985-015-0314-4
  20. Liu X, Lin CM, Annamalai T, Gao X, Lu Z, Esseili MA, Jung K, El-Tholoth M, Saif LJ, Wang Q. Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain. Vet Res 2015;46:109. https://doi.org/10.1186/s13567-015-0249-1
  21. Opriessnig T, Xiao CT, Gerber PF, Zhang J, Halbur PG. Porcine epidemic diarrhea virus RNA present in commercial spray-dried porcine plasma is not infectious to naive pigs. PLoS One 2014;9:e104766. https://doi.org/10.1371/journal.pone.0104766
  22. Chung HC, Nguyen VG, Moon HJ, Lee JH, Park SJ, Lee GE, Kim HK, Noh YS, Lee CH, Goede D, Park BK. Isolation of porcine epidemic diarrhea virus during outbreaks in South Korea, 2013-2014. Emerg Infect Dis 2015;21:2238-2240. https://doi.org/10.3201/eid2112.150437
  23. Lee S, Lee DU, Noh YH, Lee SC, Choi HW, Yang HS, Seol JH, Mun SH, Kang WM, Yoo H, Lee C. Molecular characteristics and pathogenic assessment of porcine epidemic diarrhoea virus isolates from the 2018 endemic outbreaks on Jeju Island, South Korea. Transbound Emerg Dis 2019;66:1894-1909. https://doi.org/10.1111/tbed.13219