Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Anti-inflammatory and Anti-gastritis Effect of Sogunjung-tang Mixed Soft Extract

소건중탕 혼합단미연조엑스의 항염증 및 항위염 효과

  • Kim, Myoung-Jin (National Institute for Korean Medicine Development) ;
  • Choi, Hye-Min (National Institute for Korean Medicine Development) ;
  • Nam, Won-Hee (National Institute for Korean Medicine Development) ;
  • Kim, Se-Jin (National Institute for Korean Medicine Development) ;
  • Son, Su-Mi (National Institute for Korean Medicine Development) ;
  • Kim, Jung-Ok (National Institute for Korean Medicine Development)
  • Received : 2021.08.17
  • Accepted : 2021.12.06
  • Published : 2021.12.25
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Abstract

Sogunjung-tang (SGJT) is traditional herbal prescriptions used to treat abdominal pain. In this study, we evaluated the effect on inflammation and gastritis through SGJT formulation development. SGJT composition herbal medicine was boiled in water at 95~100℃ for 3 hours and then concentrated. Sogunjung-tang mix soft extract (STM) was prepared using pharmaceutical excipients such as purified water, sodium benzoate, β-cyclodextrin and CMC-Na. Anti-inflammatory experiment was conducted using STM and lipopolysaccharide (LPS) in RAW 264.7 cells. Cell survival was measured by MTT method. Nitric oxide (NO) was measured using griess reagents, and pro inflammatory cytokines were measured by enzyme-linked immunosorbent assay and RT-PCR. Also, we verified STM validity in acute gastritis using the ICR mouse. STM was administered oral for three days. And 150 mM HCl in 60% ethanol was oral administered 0.5 mL one hour after the last drug administration. Mice were sacrificed 1 hour after 150 mM HCl in 60% ethanol administration. The gastric mucosa was visually observed. STM were not toxic in RAW 264.7 cells. Treatment of STM inhibited the production of NO and inflammatory cytokine at the protein and mRNA levels. Also, in the acute gastritis model with the mouse, the treatment of STM improved gastric mucosal bleeding and edema. In summary, it was confirmed that the treatment of STM exerts anti-inflammatory and anti-gastritis effects. Therefore, we suggest that STM may provide a preclinical evidence for the prevention and treatment of inflammatory and gastritis diseases.

Keywords

References

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