한방재활의학과학회지 (Journal of Korean Medicine Rehabilitation)

  • 제31권1호
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  • Pages.17-32
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  • 2021
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  • 1229-1854(pISSN)
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  • 2288-4114(eISSN)
한방재활의학과학회 (The society of Korean Medicine Rehabilitation)
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부자탕 추출물이 골관절염 동물 모델에 미치는 영향

Effects of Buja-tang Extract on Osteoarthritic Animal Model

  • 박중현 (대구한의대학교 한의과대학 한방재활의학과교실) ;
  • 양두화 (대구한의대학교 한의과대학 한방재활의학과교실) ;
  • 우창훈 (대구한의대학교 한의과대학 한방재활의학과교실) ;
  • 안희덕 (대구한의대학교 한의과대학 한방재활의학과교실)
  • Park, Jung-Hyun (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Daegu Haany University) ;
  • Yang, Doo-Hwa (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Daegu Haany University) ;
  • Woo, Chang-Hoon (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Daegu Haany University) ;
  • An, Hee-Duk (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Daegu Haany University)
  • 투고 : 2020.12.14
  • 심사 : 2020.12.30
  • 발행 : 2021.01.31
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초록

Objectives The present study was designed to find out the therapeutic effects and possible underlying mechanism of Buja-tang, a herbal complex formula on experimental monosodium iodoacetate (MIA)-induced osteoarthritis. Methods Osteoarthritis models were created via intra-joint injection of MIA (50 μL with 80 mg/mL) in rats. Rats were divided into five groups and each group consisted of seven. Normal group was not injected MIA and did a normal diet. Control group injected MIA and received distilled water. Indo injected MIA and oral administration of 5 mg/kg of indomethacin. BJTL injected MIA and oral administration of 100 mg/kg of Buja-tang. BJTH injected MIA and oral administration of 200 mg/kg of Buja-tang. We analyzed weight-bearing ability of hind paws, oxidative stress related factor, antioxidant protein, inflammatory protein, inflammatory messenger and cytokine in joint tissue. Pathological observation of knee cartilage tissue structures was also performed with hematoxylin & eosin and safranin-O chromosomes. Results Weight-bearing ability of hind paws showed a tendency to reduce pain. The incidence of nicotinamide adenine dinucleotide phosphate oxidase and p22phox in articular tissue was significantly reduced, and the incidence of nuclear factor-erythroid 2-related factor 2 and heme oxygenase-1 and superoxide dismutases was significantly increased. The incidence of phosphorylated inhibitor of κBα, nuclear factor-kappa B p65, inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor alpha, interleukin (IL)-6, and IL-1β decreased significantly. In pathological observation, cartilage tissue damaged by MIAs in biopsy has significantly recovered from Buja-tang administration. Conclusions Buja-tang has anti-inflammation, antioxidation and pain relief effects. So this is thought to inhibit the progress of osteoarthritis in rat caused by the MIA.

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