Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Lincomycin induces melanogenesis through the activation of MITF via p38 MAPK, AKT, and PKA signaling pathways

  • Lee, Min Suk (Jeju Inside Agency and Cosmetic Science Center, Department of Chemistry and Cosmetics, Jeju National University) ;
  • Chung, You Chul (Jeju Inside Agency and Cosmetic Science Center, Department of Chemistry and Cosmetics, Jeju National University) ;
  • Moon, Seung-Hyun (Jeju Inside Agency and Cosmetic Science Center, Department of Chemistry and Cosmetics, Jeju National University) ;
  • Hyun, Chang-Gu (Jeju Inside Agency and Cosmetic Science Center, Department of Chemistry and Cosmetics, Jeju National University)
  • Received : 2021.08.21
  • Accepted : 2021.09.17
  • Published : 2021.12.31
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Abstract

Lincomycin is a lincosamide antibiotic isolated from the actinomycete Streptomyces lincolnensis. Moreover, it has been found to be effective against infections caused by Staphylococcus, Streptococcus, and Bacteroides fragillis. To identify the melanin-inducing properties of lincomycin, we used B16F10 melanoma cells in this study. The melanin content and intracellular tyrosinase activity in the cells were increased by lincomycin, without any cytotoxicity. Western blot analysis indicated that the protein expressions of tyrosinase, tyrosinase related protein 1 (TRP1) and TRP2 increased after lincomycin treatment. In addition, lincomycin enhanced the expression of master transcription regulator of melanogenesis, a microphthalmia-associated transcription factor (MITF). Lincomycin also increased the phosphorylation of p38 mitogen-activated protein kinase (MAPK) and decreased the AKT phosphorylation. Moreover, the activation of tyrosinase activity by lincomycin was inhibited by the treatment with SB203580, which is p38 inhibitor. Furthermore, we also found that lincomycin-induced tyrosinase expression was reduced by H-89, a specific protein kinase A (PKA) inhibitor. These results indicate that lincomycin stimulate melanogenesis via MITF activation via p38 MAPK, AKT, and PKA signal pathways. Thus, lincomycin can potentially be used for treatment of hypopigmentation disorders.

Keywords

Acknowledgement

This research was supported by the Ministry of Trade, Industry & Energy (MOTIE), Korea Institute for Advancement of Technology (KIAT) through the Construction Project for Skin Clinical Solution Center (P0017663).

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