Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Inhibitory effect of n-hexane extract from Korean red ginseng marc against Streptococcus mutans causing dental caries

홍삼박 n-hexane 추출물의 충치를 유발하는 Streptococcus mutans 저해 효과

  • Kim, Dong Chung (Department of Chemical Engineering, Chungwoon University) ;
  • In, Man-Jin (Department of Chemical Engineering, Chungwoon University)
  • Received : 2021.09.15
  • Accepted : 2021.10.06
  • Published : 2021.12.31
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Abstract

In this study, to investigate the anticariogenic effect of red ginseng, the antibacterial effect, cell adherence and biofilm formation inhibitory effect of n-hexane extract of red ginseng marc (HERGM) against Streptococcus mutans, the causative bacteria of caries, were measured. The growth of S. mutans was inhibited in proportion to the concentration of HERGM, and was hardly observed at a concentration above 125 ㎍/mL (MIC =125 ㎍/mL). It was found that HERGM acts on the cell membrane and the nucleic acid component of the cell was leaked. In addition, HERGM inhibited the adherence and biofilm formation of S. mutans by more than 90% at a concentration of 125 ㎍/mL. GTase activity was completely inhibited at a concentration of 50 ㎍/mL of HERGM. In conclusion, it was found that HERGM commonly inhibited the growth and biofilm formation of S. mutans.

본 연구에서는 홍삼의 항충치 효과를 조사하기 위하여 홍삼박 n-hexane 추출물의 충치 원인균 Streptococcus mutans에 대한 항균 효과, 균체 부착과 생물막 생성 저해 효과를 측정하였다. 홍삼박 n-hexane 추출물의 농도에 비례하여 S. mutans의 생육이 저해되었으며, 125 ㎍/mL 이상의 농도에서는 거의 관찰되지 않았다(MIC=125 ㎍/mL). 홍삼박 n-hexane 추출물이 세포막에 작용하여 세포의 핵산 성분이 유출시키는 것으로 나타났다. 그리고, 홍삼박 n-hexane 추출물은 125 ㎍/mL 이상의 농도에서 S. mutans의 부착과 생물막 형성을 90% 이상 저해하였다. GTase 활성은 50 ㎍/mL 농도에서 완전히 저해되었다. 결론적으로 홍삼박 n-hexane 추출물은 S. mutans의 생육과 생물막 형성을 공통적으로 저해하는 것으로 나타났다.

Keywords

Acknowledgement

본 연구는 2021년 청운대학교 학술연구조성비의 지원으로 수행되었습니다.

References

  1. Lee HJ, Jang HD, Lee KW, Lee HJ, Kang NJ, Kim SY, Yang H (2019) Functional food. Soohaksa, Seoul
  2. Loesche WJ (1986) Role of Streptococcus mutans in human dental decay. Microbiol Rev 50: 353-380 https://doi.org/10.1128/mr.50.4.353-380.1986
  3. Bowen WH, Koo H (2011) Biology of Streptococcus mutans-derived glucosyltransferase: Role in extracellular matrix formation of cariogenic biofilms. Caries Res 45: 69-86. doi: 10.1159/000324598
  4. Jeon JG (2012) Natural product research in dental caries prevention. J Kor Dent Assoc 50: 544-551 https://doi.org/10.22974/JKDA.2012.50.9.001
  5. Choi IW, Jung CH, Park YK (2003) Anticariogenic activities of various plant extracts. Korean J Food Sci Technol 35: 1221-1225
  6. Park YM, Kim SJ, Jo KH, Yang EJ, Jung ST (2006) Anticariogenic and antioxidant activities from medicinal herbs. J Korean Soc Food Sci Nutr 35: 284-293. doi: 10.3746/jkfn.2006.35.3.284
  7. Park SN, Lim YK, Cho E, Jo E, Park PS, Kook JK (2014) Antimicrobial activity of mulberry leaf against mutans Streptococci and periodontopathogens. Int J Oral Biol 39: 201-206. doi: 10.11620/IJOB.2014.39.4.201
  8. Yoon HS, Jee YJ (2015) Inhibitory effects of coffee beans on dental caries causing Streptococcus mutans activity. Int J clin Prev Dent 11: 159-164. doi: 10.15236/ijcpd.2015.11.3.159
  9. Moon JS, Ahn JE, Han AR, Heo JS, Eom HJ, Shin CS, Choi HS, Han NS (2011) Anticariogenic activities of Lactobacillus sakei K-7 isolated from Kimchi. KSBB J 26: 513-516. doi: 10.7841/ksbbj.2011.26.6.513
  10. Choi BBR, Yun SE, Park SR, Kim GC (2020) Effectiveness of mentha extracts against oral microorganisms: an in vitro study. J Korean Acad Oral Health 44: 67-72. doi: 10.11149/jkaoh.2020.44.2.67
  11. Ren Z, Chen L, Li J, Li Y (2016) Inhibition of Streptococcus mutans polysaccharide synthesis by molecules targeting glycosyltransferase activity. J Oral Microbiol 8: 31095. doi: 10.3402/jom.v8.31095
  12. Kim SM, Choi CH, Kim JW, Won SR, Rhee HI (2008) The anticaries activity of hot water extracts from Foeniculum vulgare. J Korean Soc Appl Biol Chem 51: 84-87
  13. Kim HS, Lee SW, Sydara K, Cho SJ (2019) Antibacterial and antibiofilm activities of Diospyros malabarica stem extract against Streptococcus mutans. J Lif Sci 29: 90-96 https://doi.org/10.5352/JLS.2019.29.1.90
  14. Choi HD, Koh YJ, Choi IW, Kim YS, Park YK (2007) Anticariogenic activity and glucosyltransferase inhibitory effects of extracts from pine needle and twig. Korean J Food Sci Technol 39: 336-341
  15. Anele AS, Wu JA, Yuan CS (1999) Ginseng pharmacology: Multiple constituents and multiple actions. Biochem Pharmacol 58: 1685-1693. doi: 10.1016/s0006-2952(99)00212-9
  16. Kwak YS, Park JD, Yang JW (2003) Present and its prospect of red ginseng efficacy research. Food Ind Nutr 8: 30-37
  17. Kim YR, Yang CS (2018) Protective roles of ginseng against bacterial infection. Microbial Cell 5: 472-481. doi: 10.15698/mic2018.11.654
  18. Kim AR, Lee MS (2011) Screening of antimicrobial activity compounds from Korea ginseng fine root. J Life Sci 21: 1244-1250. doi: 10.5352/JLS.2011.21.9.1244
  19. Xue P, Yao Y, Yang XS, Feng J, Ren GX (2017) Improved antimicrobial effect of ginseng extract by heat transformation. J Ginseng Res 41: 180-187. doi: 10.1016/j.jgr.2016.03.002
  20. Son HJ, Han MS, Ryu GH (2009) Antibacterial activities of Et-OH extract from extruded white ginseng on tooth decay bacteria. J Korean Soc Food Sci Nutr 38: 951-957. doi: 10.3746/jkfn.2009.38.7.951
  21. Yun J, Kim BG, Kang JS, Park SK, Lee K, Hyun DH, Kim HM, In MJ, Kim DC (2015) Lipid-soluble ginseng extract inhibits invasion and metastasis of B16F10 melanoma cells. J Med Food 18: 102-108 https://doi.org/10.1089/jmf.2013.3138
  22. Islam B, Khan SN, Haque I, Alam M, Mushfig M, Khan AU (2008) Novel anti-adherence activity of mulberry leaves: inhibition of Streptococcus mutans biofilm by 1-deoxynojirimycin isolated from Morus alba. J Antimicrob Chemother 62: 751-757. doi: 10.1093/jac/dkn253
  23. Loo CY, Corliss DA, Ganeshkumar N (2000) Streptococcus gordonnii biofilm formation: identification of genes that code for biofilm phenotypes. J Bacteriol 182: 1374-1382. doi: 10.1128/JB.182.5.1374-1382.2000
  24. In MJ, Chae HJ, Kim DC (2014) In vitro antioxidant and anticancer potential of n-hexane extract from ginseng marc. J Appl Biol Chem 57: 247-250. doi: 10.3839/jabc.2014.039
  25. Lee J, Kim Y, Kim DC, Chae HJ (2020) Antimicrobial activities of edible plant extracts against oral bacteria. J Appl Biol Chem 63, 61-67. doi: 10.3839/jabc.2020.008
  26. Yang Y, Park BI, Hwang EH, You YO (2016) Composition analysis and inhibitory effect of Sterculia lychnophore against biofilm formation by Streptococcus mutans. Evid Based Complement Alternat Med 2016, Article ID 8163150, 9. doi: 10.1155/2016/8163150
  27. Eskandarian T, Motamedifar M, Arasteh P, Eghbali SS, Adib A, Abdoli Z (2017) Comparison of antimicrobial effects of titanium tetrafluoride, chlorohexidine, xylitol and sodium fluoride on Streptococcus mutans: An in-vitro study. Electron Physician 9: 4042-4047. doi: 10.19082/4042
  28. Bajpai VK, Sharma A, Baek KH (2013) Antibacterial mode of action of Cudrania tricuspidata fruit essential oil, affecting membrane permeability and surface characteristics of food-borne pathogens. Food Control 32: 582-590. doi: 10.1016/j.foodcont.2013.01.032
  29. Li CM, Yu JP (2014) Chemical composition, antimicrobial activity and mechanism of action of essential oil from the leaves of Macleaya cordata (Willd.) R. Br. J Food Safety 35: 227-236. doi: 10.1111/jfs.12175
  30. Limsong J, Benjavongkulchai S, Kuvatanasuchati J (2004) Inhibitory effect of some herbal extracts on adherence of Streptococcus mutans. J Ethnopharmacol 92: 281-289. doi: 10.1016/j.jep.2004.03.008
  31. Yoon Y, Choi KH (2010) Identification of inhibitory effect on Streptococcus mutans by oleanolic acid. J Life Sci 20: 321-325. doi: 10.5352/JLS.2010.20.3.321
  32. Abedian Z, Jenabian N, Moghadamnia AA, Zabihi E, Tashakorian H, Rajabnia M, Sadighian F, Bijani A (2019) Antibacterial activity of high-molecular-weight and low-molecular-weight chitosan upon oral pathogens. J Conserv Dent 22: 169-174 https://doi.org/10.4103/jcd.jcd_300_18
  33. Chen X, Baliri EBM, Kim N, Kim JR, Yoo D, Oh DH (2020) Microbial etiology and prevention of dental caries: Exploiting natural products to inhibit cariogenic biofilms. Pathogens 9, 569. doi:10.3390/pathogens9070569
  34. Moon KH, Lee YC, Kim JN (2019) Effects of foreign plant extracts on cell growth and biofilm formation of Streptococcus mutans. J Life Sci 29: 712-723. doi: 10.5352/JLS.2019.29.6.712
  35. Ooshima T, Matsumura M, Hoshino T, Kawabata S, Sobue S, Fujiwara T (2001) Contributions of three glycosyltransferase to sucrose-dependent adherence of Streptococcus mutans. J Dent Res 80: 1672-1677 https://doi.org/10.1177/00220345010800071401
  36. Yoon SY, Kim SH, Chung HL, Lee JJ, Huh CS, Baek YJ (2000) Anicariogenic effects of unripe apple extract. Korean J Food Sci Technol 32, 168-173