Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Anti-adipogenic Effects of Vibration with Varied Frequencies on 3T3-L1 Preadipocytes

3T3-L1 지방전구세포에 대한 다양한 주파수 진동의 지방 생성 억제 효과

  • Lee, Yeong Hun (Department of Biomedical Engineering, Yonsei University) ;
  • Lee, Seok-Ho (Department of Biomedical Engineering, Yonsei University) ;
  • Jung, Haebin (Department of Biomedical Engineering, Yonsei University) ;
  • Jung, Yong Chan (Department of Biomedical Engineering, Yonsei University) ;
  • Kim, Min Hwan (Department of Biomedical Engineering, Yonsei University) ;
  • Lee, Eun Mi (Department of Biomedical Engineering, Yonsei University) ;
  • Kim, Chi Hyun (Department of Biomedical Engineering, Yonsei University)
  • 이영훈 (연세대학교 보건과학대학 의공학과) ;
  • 이석호 (연세대학교 보건과학대학 의공학과) ;
  • 정혜빈 (연세대학교 보건과학대학 의공학과) ;
  • 정용찬 (연세대학교 보건과학대학 의공학과) ;
  • 김민환 (연세대학교 보건과학대학 의공학과) ;
  • 이은미 (연세대학교 보건과학대학 의공학과) ;
  • 김지현 (연세대학교 보건과학대학 의공학과)
  • Received : 2020.11.20
  • Accepted : 2021.02.01
  • Published : 2021.02.28
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Abstract

Vibration is a mechanical cue that can be applied to adipose tissues for the purpose of treating obesity. However, the exact correlation between vibration and other anti-adipogenic pathways, such as development of cytoskeleton and apoptosis, remains unknown. The objective of this study was to investigate the unknown anti-adipogenic effects of vibration with varied frequencies on preadipocytes. 3T3-L1 preadipocytes were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 5% calf serum at 37 ℃ with 5% CO2 in a humidified incubator. Vibration was generated using Arduino Uno microcontroller and vibration motor module with 1 V DC, and applied to preadipocytes for 3 days. Frequency conditions were set to 20, 55, and 90 Hz. Then, the expressions of p38 pathway, ROCK-1, α-actinin, Bax, Bcl-2, caspase-9, 8, and 3 were analyzed with western blot. As a result, p38 pathway was inhibited in 55 and 90 Hz while ROCK-1 and α-actinin were expressed in 20 Hz. Caspase-3, a terminal apoptotic factor, was activated in 20 Hz via extrinsic pathway rather than intrinsic pathway. Results suggest that various frequencies of vibration can inhibit adipogenesis via different pathways which sheds light on future mechanotransduction applications of vibration for the treatment of obesity.

Keywords

References

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